Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury

R.C. Lai, F. Arslan, M.M. Lee, N.S. Sze, A. Choo, T.S. Chen, M. Salto-Tellez, L. Timmers, C.N. Lee, R.M. El Oakley, G. Pasterkamp, D.P.V. de Kleijn, S.K. Lim

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Human ESC-derived mesenchymal stem cell (MSC)-conditioned medium (CM) was previously shown to mediate cardioprotection during myocardial ischemia/reperfusion injury through large complexes of 50-100 nm. Here we show that these MSCs secreted 50- to 100-nm particles. These particles could be visualized by electron microscopy and were shown to be phospholipid vesicles consisting of cholesterol, sphingomyelin, and phosphatidylcholine. They contained coimmunoprecipitating exosome-associated proteins, e.g., CD81, CD9, and Alix. These particles were purified as a homogeneous population of particles with a hydrodynamic radius of 55-65 nm by size-exclusion fractionation on a HPLC. Together these observations indicated that these particles are exosomes. These purified exosomes reduced infarct size in a mouse model of myocardial ischemia/reperfusion injury. Therefore, MSC mediated its cardioprotective paracrine effect by secreting exosomes. This novel role of exosomes highlights a new perspective into intercellular mediation of tissue injury and repair, and engenders novel approaches to the development of biologics for tissue repair.

Original languageEnglish
Pages (from-to)214-222
Number of pages9
JournalStem Cell Research
Volume4
Issue number3
DOIs
Publication statusPublished - 2010

Keywords

  • Animals
  • Antigens, CD
  • Antigens, CD81
  • Antigens, CD9
  • Calcium-Binding Proteins
  • Cardiotonic Agents
  • Chromatography, High Pressure Liquid
  • Disease Models, Animal
  • Exosomes
  • Humans
  • Membrane Glycoproteins
  • Mesenchymal Stromal Cells
  • Mice
  • Microscopy, Electron
  • Myocardial Ischemia
  • Reperfusion Injury
  • Journal Article

Fingerprint

Dive into the research topics of 'Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury'. Together they form a unique fingerprint.

Cite this