Evaluation of the use of RT-PCR for the early diagnosis of dengue fever

MP Grobusch; M Niedrig; K Gobels; K Klipstein-Grobusch; D Teichmann

doi:10.1111/j.1469-0691.2006.01353.x

Evaluation of the use of RT-PCR for the early diagnosis of dengue fever

MP Grobusch^*, M Niedrig, K Gobels, K Klipstein-Grobusch, D Teichmann

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

RT-PCR was used to diagnose dengue virus infections confirmed serologically in 26 returning travellers. RT-PCR was positive for three (75%) of four samples taken on or before day 3 of the illness, for 15 (78.9%) of 19 samples taken between days 4 and 7, and for none of three samples tested on or after day 8 (p 0.0337). When applied early, RT-PCR seems to be a useful tool for the diagnosis of dengue fever.

Original language	English
Pages (from-to)	395-397
Number of pages	3
Journal	Clinical Microbiology and Infection
Volume	12
Issue number	4
DOIs	https://doi.org/10.1111/j.1469-0691.2006.01353.x
Publication status	Published - Apr 2006
Externally published	Yes

Keywords

dengue fever
diagnosis
RT-PCR
sampling time
sensitivity
QUALITY-CONTROL ASSESSMENT
VIRUS-INFECTIONS
HEMORRHAGIC-FEVER
TRAVELERS
VIREMIA
REGION

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10.1111/j.1469-0691.2006.01353.x

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title = "Evaluation of the use of RT-PCR for the early diagnosis of dengue fever",

abstract = "RT-PCR was used to diagnose dengue virus infections confirmed serologically in 26 returning travellers. RT-PCR was positive for three (75%) of four samples taken on or before day 3 of the illness, for 15 (78.9%) of 19 samples taken between days 4 and 7, and for none of three samples tested on or after day 8 (p 0.0337). When applied early, RT-PCR seems to be a useful tool for the diagnosis of dengue fever.",

keywords = "dengue fever, diagnosis, RT-PCR, sampling time, sensitivity, QUALITY-CONTROL ASSESSMENT, VIRUS-INFECTIONS, HEMORRHAGIC-FEVER, TRAVELERS, VIREMIA, REGION",

author = "MP Grobusch and M Niedrig and K Gobels and K Klipstein-Grobusch and D Teichmann",

year = "2006",

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doi = "10.1111/j.1469-0691.2006.01353.x",

language = "English",

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T1 - Evaluation of the use of RT-PCR for the early diagnosis of dengue fever

AU - Grobusch, MP

AU - Niedrig, M

AU - Gobels, K

AU - Klipstein-Grobusch, K

AU - Teichmann, D

PY - 2006/4

Y1 - 2006/4

N2 - RT-PCR was used to diagnose dengue virus infections confirmed serologically in 26 returning travellers. RT-PCR was positive for three (75%) of four samples taken on or before day 3 of the illness, for 15 (78.9%) of 19 samples taken between days 4 and 7, and for none of three samples tested on or after day 8 (p 0.0337). When applied early, RT-PCR seems to be a useful tool for the diagnosis of dengue fever.

AB - RT-PCR was used to diagnose dengue virus infections confirmed serologically in 26 returning travellers. RT-PCR was positive for three (75%) of four samples taken on or before day 3 of the illness, for 15 (78.9%) of 19 samples taken between days 4 and 7, and for none of three samples tested on or after day 8 (p 0.0337). When applied early, RT-PCR seems to be a useful tool for the diagnosis of dengue fever.

KW - dengue fever

KW - diagnosis

KW - RT-PCR

KW - sampling time

KW - sensitivity

KW - QUALITY-CONTROL ASSESSMENT

KW - VIRUS-INFECTIONS

KW - HEMORRHAGIC-FEVER

KW - TRAVELERS

KW - VIREMIA

KW - REGION

U2 - 10.1111/j.1469-0691.2006.01353.x

DO - 10.1111/j.1469-0691.2006.01353.x

M3 - Article

C2 - 16524419

SN - 1198-743X

VL - 12

SP - 395

EP - 397

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

IS - 4

ER -

Evaluation of the use of RT-PCR for the early diagnosis of dengue fever

Abstract

Keywords

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