Evaluation of different techniques for detection of methicillin resistance in Staphylococcus aureus

K. Trzcinski; J. Boruc; Z. Kowalik; S. Tyski; T. Zareba; W. Hryniewicz

Evaluation of different techniques for detection of methicillin resistance in Staphylococcus aureus

K. Trzcinski^*
, J. Boruc
, Z. Kowalik
, S. Tyski
, T. Zareba
, W. Hryniewicz

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

Resistance to methicillin of 70 Staphylococcus aureus isolates from infection and 6 standard strains was evaluated by screening and disc diffusion techniques. Amongst wild S. aureus isolates 28 were identified as methicillin-susceptible (MSSA), 18 as heterogeneously resistant and 24 as homogeneously resistant to methicillin (MRSA). The best detection of methicillin-resistance was obtained by two screening techniques 1) on Mueller-Hinton agar with 6 mg oxacillin per litre and incubation for 40 hours at 37°C. The evaluation of commercial test Crystal MRSA ID System proved its high usefulness in proper detection of MRSA and allowed to get results in 4 hours. Disk diffusion with 1 μg oxacillin disk and incubation for 20 hours at 30°C proof to be the most reliable out of all disc diffusion techniques studied although inferior to screening techniques.

Original language	English
Pages (from-to)	113-131
Number of pages	19
Journal	Medycyna Doswiadczalna i Mikrobiologia
Volume	46
Issue number	3
Publication status	Published - 1 Jan 1994

Cite this

@article{b73e8f76a5924099b8f0228f2d026da3,

title = "Evaluation of different techniques for detection of methicillin resistance in Staphylococcus aureus",

abstract = "Resistance to methicillin of 70 Staphylococcus aureus isolates from infection and 6 standard strains was evaluated by screening and disc diffusion techniques. Amongst wild S. aureus isolates 28 were identified as methicillin-susceptible (MSSA), 18 as heterogeneously resistant and 24 as homogeneously resistant to methicillin (MRSA). The best detection of methicillin-resistance was obtained by two screening techniques 1) on Mueller-Hinton agar with 6 mg oxacillin per litre and incubation for 40 hours at 37°C. The evaluation of commercial test Crystal MRSA ID System proved its high usefulness in proper detection of MRSA and allowed to get results in 4 hours. Disk diffusion with 1 μg oxacillin disk and incubation for 20 hours at 30°C proof to be the most reliable out of all disc diffusion techniques studied although inferior to screening techniques.",

author = "K. Trzcinski and J. Boruc and Z. Kowalik and S. Tyski and T. Zareba and W. Hryniewicz",

year = "1994",

month = jan,

day = "1",

language = "English",

volume = "46",

pages = "113--131",

journal = "Medycyna Doswiadczalna i Mikrobiologia",

issn = "0025-8601",

publisher = "Panstwowy Zaklad Higieny",

number = "3",

}

TY - JOUR

T1 - Evaluation of different techniques for detection of methicillin resistance in Staphylococcus aureus

AU - Trzcinski, K.

AU - Boruc, J.

AU - Kowalik, Z.

AU - Tyski, S.

AU - Zareba, T.

AU - Hryniewicz, W.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Resistance to methicillin of 70 Staphylococcus aureus isolates from infection and 6 standard strains was evaluated by screening and disc diffusion techniques. Amongst wild S. aureus isolates 28 were identified as methicillin-susceptible (MSSA), 18 as heterogeneously resistant and 24 as homogeneously resistant to methicillin (MRSA). The best detection of methicillin-resistance was obtained by two screening techniques 1) on Mueller-Hinton agar with 6 mg oxacillin per litre and incubation for 40 hours at 37°C. The evaluation of commercial test Crystal MRSA ID System proved its high usefulness in proper detection of MRSA and allowed to get results in 4 hours. Disk diffusion with 1 μg oxacillin disk and incubation for 20 hours at 30°C proof to be the most reliable out of all disc diffusion techniques studied although inferior to screening techniques.

AB - Resistance to methicillin of 70 Staphylococcus aureus isolates from infection and 6 standard strains was evaluated by screening and disc diffusion techniques. Amongst wild S. aureus isolates 28 were identified as methicillin-susceptible (MSSA), 18 as heterogeneously resistant and 24 as homogeneously resistant to methicillin (MRSA). The best detection of methicillin-resistance was obtained by two screening techniques 1) on Mueller-Hinton agar with 6 mg oxacillin per litre and incubation for 40 hours at 37°C. The evaluation of commercial test Crystal MRSA ID System proved its high usefulness in proper detection of MRSA and allowed to get results in 4 hours. Disk diffusion with 1 μg oxacillin disk and incubation for 20 hours at 30°C proof to be the most reliable out of all disc diffusion techniques studied although inferior to screening techniques.

UR - https://www.scopus.com/pages/publications/0028019102

M3 - Article

C2 - 7996929

AN - SCOPUS:0028019102

SN - 0025-8601

VL - 46

SP - 113

EP - 131

JO - Medycyna Doswiadczalna i Mikrobiologia

JF - Medycyna Doswiadczalna i Mikrobiologia

IS - 3

ER -

Evaluation of different techniques for detection of methicillin resistance in Staphylococcus aureus

Abstract

Other files and links

Fingerprint

Cite this