Abstract
Resistance to methicillin of 70 Staphylococcus aureus isolates from infection and 6 standard strains was evaluated by screening and disc diffusion techniques. Amongst wild S. aureus isolates 28 were identified as methicillin-susceptible (MSSA), 18 as heterogeneously resistant and 24 as homogeneously resistant to methicillin (MRSA). The best detection of methicillin-resistance was obtained by two screening techniques 1) on Mueller-Hinton agar with 6 mg oxacillin per litre and incubation for 40 hours at 37°C. The evaluation of commercial test Crystal MRSA ID System proved its high usefulness in proper detection of MRSA and allowed to get results in 4 hours. Disk diffusion with 1 μg oxacillin disk and incubation for 20 hours at 30°C proof to be the most reliable out of all disc diffusion techniques studied although inferior to screening techniques.
| Original language | English |
|---|---|
| Pages (from-to) | 113-131 |
| Number of pages | 19 |
| Journal | Medycyna Doswiadczalna i Mikrobiologia |
| Volume | 46 |
| Issue number | 3 |
| Publication status | Published - 1 Jan 1994 |