Evaluation of different techniques for detection of methicillin resistance in Staphylococcus aureus

K. Trzcinski*, J. Boruc, Z. Kowalik, S. Tyski, T. Zareba, W. Hryniewicz

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Resistance to methicillin of 70 Staphylococcus aureus isolates from infection and 6 standard strains was evaluated by screening and disc diffusion techniques. Amongst wild S. aureus isolates 28 were identified as methicillin-susceptible (MSSA), 18 as heterogeneously resistant and 24 as homogeneously resistant to methicillin (MRSA). The best detection of methicillin-resistance was obtained by two screening techniques 1) on Mueller-Hinton agar with 6 mg oxacillin per litre and incubation for 40 hours at 37°C. The evaluation of commercial test Crystal MRSA ID System proved its high usefulness in proper detection of MRSA and allowed to get results in 4 hours. Disk diffusion with 1 μg oxacillin disk and incubation for 20 hours at 30°C proof to be the most reliable out of all disc diffusion techniques studied although inferior to screening techniques.

Original languageEnglish
Pages (from-to)113-131
Number of pages19
JournalMedycyna Doswiadczalna i Mikrobiologia
Volume46
Issue number3
Publication statusPublished - 1 Jan 1994

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