Evaluation of Borrelia real time PCR DNA targeting OspA, FlaB and 5S-23S IGS and Borrelia 16S rRNA RT-qPCR

B.H. de Leeuw; B. Maraha; L. Hollemans; H. Sprong; A.H. Brandenburg; P.J. Westenend; J.G. Kusters

doi:10.1016/j.mimet.2014.09.001

Evaluation of Borrelia real time PCR DNA targeting OspA, FlaB and 5S-23S IGS and Borrelia 16S rRNA RT-qPCR

B.H. de Leeuw
, B. Maraha
, L. Hollemans
, H. Sprong
, A.H. Brandenburg
, P.J. Westenend
, J.G. Kusters

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Borrelia burgdorferi non-sensu lato (s.l.) strains occurred in the Netherlands. A multiplex OspA, FlaB, IGS real time PCR was compared to 16S rRNA/rDNA RT-qPCR with lower average Cycle threshold (Ct) and LOD on strain dilutions. Multiplexing increased sensitivity on CSF samples (n=74), distinguishing B. burgdorferi s.l. from non-s.l. strains.

Original language	English
Pages (from-to)	41-46
Number of pages	6
Journal	Journal of Microbiological Methods
Volume	107
DOIs	https://doi.org/10.1016/j.mimet.2014.09.001
Publication status	Published - Dec 2014

Keywords

Adult
Aged
Antigens, Surface
Bacterial Outer Membrane Proteins
Bacterial Vaccines
Borrelia
DNA, Ribosomal Spacer
Female
Flagellin
Humans
Lipoproteins
Lyme Disease
Male
Middle Aged
Multiplex Polymerase Chain Reaction
RNA, Ribosomal
Real-Time Polymerase Chain Reaction
Reproducibility of Results
Sensitivity and Specificity

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10.1016/j.mimet.2014.09.001

Cite this

@article{e374e91f589e428cbf93658c3663308d,

title = "Evaluation of Borrelia real time PCR DNA targeting OspA, FlaB and 5S-23S IGS and Borrelia 16S rRNA RT-qPCR",

abstract = "Borrelia burgdorferi non-sensu lato (s.l.) strains occurred in the Netherlands. A multiplex OspA, FlaB, IGS real time PCR was compared to 16S rRNA/rDNA RT-qPCR with lower average Cycle threshold (Ct) and LOD on strain dilutions. Multiplexing increased sensitivity on CSF samples (n=74), distinguishing B. burgdorferi s.l. from non-s.l. strains.",

keywords = "Adult, Aged, Antigens, Surface, Bacterial Outer Membrane Proteins, Bacterial Vaccines, Borrelia, DNA, Ribosomal Spacer, Female, Flagellin, Humans, Lipoproteins, Lyme Disease, Male, Middle Aged, Multiplex Polymerase Chain Reaction, RNA, Ribosomal, Real-Time Polymerase Chain Reaction, Reproducibility of Results, Sensitivity and Specificity",

author = "\{de Leeuw\}, B.H. and B. Maraha and L. Hollemans and H. Sprong and A.H. Brandenburg and P.J. Westenend and J.G. Kusters",

year = "2014",

month = dec,

doi = "10.1016/j.mimet.2014.09.001",

language = "English",

volume = "107",

pages = "41--46",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

}

TY - JOUR

T1 - Evaluation of Borrelia real time PCR DNA targeting OspA, FlaB and 5S-23S IGS and Borrelia 16S rRNA RT-qPCR

AU - de Leeuw, B.H.

AU - Maraha, B.

AU - Hollemans, L.

AU - Sprong, H.

AU - Brandenburg, A.H.

AU - Westenend, P.J.

AU - Kusters, J.G.

PY - 2014/12

Y1 - 2014/12

N2 - Borrelia burgdorferi non-sensu lato (s.l.) strains occurred in the Netherlands. A multiplex OspA, FlaB, IGS real time PCR was compared to 16S rRNA/rDNA RT-qPCR with lower average Cycle threshold (Ct) and LOD on strain dilutions. Multiplexing increased sensitivity on CSF samples (n=74), distinguishing B. burgdorferi s.l. from non-s.l. strains.

AB - Borrelia burgdorferi non-sensu lato (s.l.) strains occurred in the Netherlands. A multiplex OspA, FlaB, IGS real time PCR was compared to 16S rRNA/rDNA RT-qPCR with lower average Cycle threshold (Ct) and LOD on strain dilutions. Multiplexing increased sensitivity on CSF samples (n=74), distinguishing B. burgdorferi s.l. from non-s.l. strains.

KW - Adult

KW - Aged

KW - Antigens, Surface

KW - Bacterial Outer Membrane Proteins

KW - Bacterial Vaccines

KW - Borrelia

KW - DNA, Ribosomal Spacer

KW - Female

KW - Flagellin

KW - Humans

KW - Lipoproteins

KW - Lyme Disease

KW - Male

KW - Middle Aged

KW - Multiplex Polymerase Chain Reaction

KW - RNA, Ribosomal

KW - Real-Time Polymerase Chain Reaction

KW - Reproducibility of Results

KW - Sensitivity and Specificity

U2 - 10.1016/j.mimet.2014.09.001

DO - 10.1016/j.mimet.2014.09.001

M3 - Article

C2 - 25218111

SN - 0167-7012

VL - 107

SP - 41

EP - 46

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

ER -

Evaluation of Borrelia real time PCR DNA targeting OspA, FlaB and 5S-23S IGS and Borrelia 16S rRNA RT-qPCR

Abstract

Keywords

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