Evaluation of a Legionella urinary antigen enzyme immunoassay for rapid detection of Legionella pneumophila in water samples

S. Blanco; C. Prat; M. D. Sánchez; D. Ferrer; T. Pellicer; L. Haba; I. Latorre; C. Vilaplana; V. Ausina; J. Domínguez

doi:10.1016/j.ijheh.2007.02.002

Evaluation of a Legionella urinary antigen enzyme immunoassay for rapid detection of Legionella pneumophila in water samples

S. Blanco, C. Prat, M. D. Sánchez, D. Ferrer, T. Pellicer, L. Haba, I. Latorre, C. Vilaplana, V. Ausina, J. Domínguez^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

Despite advances in medium formulations and pretreatment techniques, recovery of Legionella from water samples can still be quite low, difficult and time consuming. The aim of this study was to evaluate the utility of a Legionella urinary antigen enzyme immunoassay (Bartels ELISA, Trinity Biotech, Ireland) for the detection of Legionella in water samples. Reference ATCC Legionella strains were used to spike water samples to a final concentration of 10⁴-10⁵ cfu/ml. The lower detection limit of the test for all Legionella pneumophila serogroups was assessed by serial dilutions of spiked water samples. Legionella antigen was detected in all filtered samples except for those spiked with L. bozemanii and L. longbeachae. The lower detection limit for soluble L. pneumophila serogroup 1 antigen was 780 cfu/ml. Bartels ELISA could be a useful method for antigen detection in water samples when a high recovery of L. pneumophila is suspected. The test could be used as a rapid screening method for the detection of Legionella in a large number of samples. However, the low sensitivity of the test requires to keep on performing conventional culture for isolation and for further studies on isolated bacteria.

Original language	English
Pages (from-to)	168-171
Number of pages	4
Journal	International Journal of Hygiene and Environmental Health
Volume	211
Issue number	1-2
DOIs	https://doi.org/10.1016/j.ijheh.2007.02.002
Publication status	Published - 12 Mar 2008
Externally published	Yes

Keywords

Bartels ELISA
Legionella antigen
Water samples

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10.1016/j.ijheh.2007.02.002

Cite this

Blanco, S., Prat, C., Sánchez, M. D., Ferrer, D., Pellicer, T., Haba, L., Latorre, I., Vilaplana, C., Ausina, V., & Domínguez, J. (2008). Evaluation of a Legionella urinary antigen enzyme immunoassay for rapid detection of Legionella pneumophila in water samples. International Journal of Hygiene and Environmental Health, 211(1-2), 168-171. https://doi.org/10.1016/j.ijheh.2007.02.002

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title = "Evaluation of a Legionella urinary antigen enzyme immunoassay for rapid detection of Legionella pneumophila in water samples",

abstract = "Despite advances in medium formulations and pretreatment techniques, recovery of Legionella from water samples can still be quite low, difficult and time consuming. The aim of this study was to evaluate the utility of a Legionella urinary antigen enzyme immunoassay (Bartels ELISA, Trinity Biotech, Ireland) for the detection of Legionella in water samples. Reference ATCC Legionella strains were used to spike water samples to a final concentration of 104-105 cfu/ml. The lower detection limit of the test for all Legionella pneumophila serogroups was assessed by serial dilutions of spiked water samples. Legionella antigen was detected in all filtered samples except for those spiked with L. bozemanii and L. longbeachae. The lower detection limit for soluble L. pneumophila serogroup 1 antigen was 780 cfu/ml. Bartels ELISA could be a useful method for antigen detection in water samples when a high recovery of L. pneumophila is suspected. The test could be used as a rapid screening method for the detection of Legionella in a large number of samples. However, the low sensitivity of the test requires to keep on performing conventional culture for isolation and for further studies on isolated bacteria.",

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Blanco, S, Prat, C, Sánchez, MD, Ferrer, D, Pellicer, T, Haba, L, Latorre, I, Vilaplana, C, Ausina, V & Domínguez, J 2008, 'Evaluation of a Legionella urinary antigen enzyme immunoassay for rapid detection of Legionella pneumophila in water samples', International Journal of Hygiene and Environmental Health, vol. 211, no. 1-2, pp. 168-171. https://doi.org/10.1016/j.ijheh.2007.02.002

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AU - Blanco, S.

AU - Prat, C.

AU - Sánchez, M. D.

AU - Ferrer, D.

AU - Pellicer, T.

AU - Haba, L.

AU - Latorre, I.

AU - Vilaplana, C.

AU - Ausina, V.

AU - Domínguez, J.

PY - 2008/3/12

Y1 - 2008/3/12

N2 - Despite advances in medium formulations and pretreatment techniques, recovery of Legionella from water samples can still be quite low, difficult and time consuming. The aim of this study was to evaluate the utility of a Legionella urinary antigen enzyme immunoassay (Bartels ELISA, Trinity Biotech, Ireland) for the detection of Legionella in water samples. Reference ATCC Legionella strains were used to spike water samples to a final concentration of 104-105 cfu/ml. The lower detection limit of the test for all Legionella pneumophila serogroups was assessed by serial dilutions of spiked water samples. Legionella antigen was detected in all filtered samples except for those spiked with L. bozemanii and L. longbeachae. The lower detection limit for soluble L. pneumophila serogroup 1 antigen was 780 cfu/ml. Bartels ELISA could be a useful method for antigen detection in water samples when a high recovery of L. pneumophila is suspected. The test could be used as a rapid screening method for the detection of Legionella in a large number of samples. However, the low sensitivity of the test requires to keep on performing conventional culture for isolation and for further studies on isolated bacteria.

AB - Despite advances in medium formulations and pretreatment techniques, recovery of Legionella from water samples can still be quite low, difficult and time consuming. The aim of this study was to evaluate the utility of a Legionella urinary antigen enzyme immunoassay (Bartels ELISA, Trinity Biotech, Ireland) for the detection of Legionella in water samples. Reference ATCC Legionella strains were used to spike water samples to a final concentration of 104-105 cfu/ml. The lower detection limit of the test for all Legionella pneumophila serogroups was assessed by serial dilutions of spiked water samples. Legionella antigen was detected in all filtered samples except for those spiked with L. bozemanii and L. longbeachae. The lower detection limit for soluble L. pneumophila serogroup 1 antigen was 780 cfu/ml. Bartels ELISA could be a useful method for antigen detection in water samples when a high recovery of L. pneumophila is suspected. The test could be used as a rapid screening method for the detection of Legionella in a large number of samples. However, the low sensitivity of the test requires to keep on performing conventional culture for isolation and for further studies on isolated bacteria.

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ER -

Evaluation of a Legionella urinary antigen enzyme immunoassay for rapid detection of Legionella pneumophila in water samples

Abstract

Keywords

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