TY - JOUR
T1 - Evaluating the non-tuberculous mycobacteria effect in the tuberculosis infection diagnosis
AU - Latorre, I.
AU - De Souza-Galvão, M.
AU - Ruiz-Manzano, J.
AU - Lacoma, A.
AU - Prat, C.
AU - Altet, N.
AU - Ausina, V.
AU - Domínguez, J.
PY - 2010/2
Y1 - 2010/2
N2 - The aim of the present study was to determine the role of previous non-tuberculous mycobacteria sensitisation in children as a factor of discordant results between tuberculin skin test (TST) and an in vitro T-cell based assay (T-SPOT.TB; Oxford Immunotec, Oxford, UK). Weenrolled 21 non-bacille Calmette-Guérin-vaccinated paediatric patients for suspicious of latent tuberculosis infection (LTBI). These patients yielded a positive TST and a negative T-SPOT.TB. Cellswere stimulated with Mycobacterium aviumsensitin (having cross-reaction with Mycobacterium intracellulare and Mycobacterium scrofulaceum) and the presence of reactive T-cells was determined by an ex vivo ELISPOT. From the 21 patients, in 10 cases (47.6%), we obtained a positive ELISPOT result after stimulation with M. avium sensitin, in six (28.6%) cases, the result was negative and in the remaining five (23.8%) cases, the result was indeterminate. In conclusion, previous non-tuberculous mycobacteria sensitisation induces false-positive results in the TST for diagnosing LTBI and the use of γ-interferon tests could avoid unnecessary chemoprophylaxis treatment among a child population. Copyright
AB - The aim of the present study was to determine the role of previous non-tuberculous mycobacteria sensitisation in children as a factor of discordant results between tuberculin skin test (TST) and an in vitro T-cell based assay (T-SPOT.TB; Oxford Immunotec, Oxford, UK). Weenrolled 21 non-bacille Calmette-Guérin-vaccinated paediatric patients for suspicious of latent tuberculosis infection (LTBI). These patients yielded a positive TST and a negative T-SPOT.TB. Cellswere stimulated with Mycobacterium aviumsensitin (having cross-reaction with Mycobacterium intracellulare and Mycobacterium scrofulaceum) and the presence of reactive T-cells was determined by an ex vivo ELISPOT. From the 21 patients, in 10 cases (47.6%), we obtained a positive ELISPOT result after stimulation with M. avium sensitin, in six (28.6%) cases, the result was negative and in the remaining five (23.8%) cases, the result was indeterminate. In conclusion, previous non-tuberculous mycobacteria sensitisation induces false-positive results in the TST for diagnosing LTBI and the use of γ-interferon tests could avoid unnecessary chemoprophylaxis treatment among a child population. Copyright
KW - Childhood
KW - ELISPOT
KW - Interferon-γ release assays
KW - Latent tuberculosis infection
KW - Mycobacterium avium sensitin
KW - Non-tuberculous mycobacteria
UR - https://www.scopus.com/pages/publications/76149100611
U2 - 10.1183/09031936.00196608
DO - 10.1183/09031936.00196608
M3 - Article
C2 - 20123845
AN - SCOPUS:76149100611
SN - 0903-1936
VL - 35
SP - 338
EP - 342
JO - European Respiratory Journal
JF - European Respiratory Journal
IS - 2
ER -