TY - JOUR
T1 - Establishment and Culture of Human Intestinal Organoids Derived from Adult Stem Cells
AU - Pleguezuelos-Manzano, Cayetano
AU - Puschhof, Jens
AU - van den Brink, Stieneke
AU - Geurts, Veerle
AU - Beumer, Joep
AU - Clevers, Hans
N1 - Funding Information:
We thank M. Bannier‐Hélaouët for critical reading of the manuscript and comments. Development of this method was supported by CRUK grant OPTIMISTICC (C10674/A27140) (C.P.‐M., J.P.), the Gravitation projects CancerGenomiCs.nl, and the Netherlands Organ‐on‐Chip Initiative (024.003.001) from the Netherlands Organisation for Scientific Research (NWO) funded by the Ministry of Education, Culture and Science of the government of the Netherlands (C.P.‐M., J.P.), the Oncode Institute (partly financed by the Dutch Cancer Society), the European Research Council under ERC Advanced Grant Agreement no. 67013 (J.P., H.C.) and NETRF/Petersen Accelerator (J.B.).
Funding Information:
We thank M. Bannier-H?laou?t for critical reading of the manuscript and comments. Development of this method was supported by CRUK grant OPTIMISTICC (C10674/A27140) (C.P.-M., J.P.), the Gravitation projects CancerGenomiCs.nl, and the Netherlands Organ-on-Chip Initiative (024.003.001) from the Netherlands Organisation for Scientific Research (NWO) funded by the Ministry of Education, Culture and Science of the government of the Netherlands (C.P.-M., J.P.), the Oncode Institute (partly financed by the Dutch Cancer Society), the European Research Council under ERC Advanced Grant Agreement no. 67013 (J.P., H.C.) and NETRF/Petersen Accelerator (J.B.).
Publisher Copyright:
© 2020 The Authors.
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Human intestinal organoids derived from adult stem cells are miniature ex vivo versions of the human intestinal epithelium. Intestinal organoids are useful tools for the study of intestinal physiology as well as many disease conditions. These organoids present numerous advantages compared to immortalized cell lines, but working with them requires dedicated techniques. The protocols described in this article provide a basic guide to establishment and maintenance of human intestinal organoids derived from small intestine and colon biopsies. Additionally, this article provides an overview of several downstream applications of human intestinal organoids.
AB - Human intestinal organoids derived from adult stem cells are miniature ex vivo versions of the human intestinal epithelium. Intestinal organoids are useful tools for the study of intestinal physiology as well as many disease conditions. These organoids present numerous advantages compared to immortalized cell lines, but working with them requires dedicated techniques. The protocols described in this article provide a basic guide to establishment and maintenance of human intestinal organoids derived from small intestine and colon biopsies. Additionally, this article provides an overview of several downstream applications of human intestinal organoids.
KW - adult stem cells
KW - human intestinal organoids
KW - organoid cryopreservation
KW - organoid culture establishment
KW - organoid differentiation
KW - organoid immunofluorescence
KW - organoid passage
KW - single-cell clonal organoid culture
KW - specialized organoid reagents
UR - https://www.scopus.com/pages/publications/85091129162
U2 - 10.1002/cpim.106
DO - 10.1002/cpim.106
M3 - Article
C2 - 32940424
SN - 1934-3671
VL - 130
JO - Current protocols in immunology
JF - Current protocols in immunology
IS - 1
M1 - e106
ER -