Abstract
We studied 44 cases of Hodgkin's disease for the presence of Epstein-Barr virus (EBV) DNA, its localization and the expression of the EBV receptor on the tumour cells. EBV DNA was found in 52% (16/31) of the Hodgkin's lymphomas using the polymerase chain reaction. With a very sensitive non-radioactive DNA in situ hybridization technique in combination with immunohistochemistry for CD 30 or CD 15 antigens, EBV DNA was localized to Reed-Sternberg cells and its mononuclear variants. The relationship between the presence of EBV DNA and the expression of the EBV-receptor CR2 (CD 21) on Reed-Sternberg cells was studied using the same techniques and two different monoclonal anti-CD 21 antibodies. CR2 could be detected on a substantial number of the Reed-Sternberg cells in EBV DNA positive Hodgkin's lymphomas (9/12; 75%), whereas in EBV negative cases positivity with anti-CD 21 was rare (1/13; 8%). The results indicate that CR2 expression on Reed-Sternberg cells and the presence of EBV DNA sequences are frequently associated in Hodgkin's lymphomas.
| Original language | English |
|---|---|
| Pages (from-to) | 51-7 |
| Number of pages | 7 |
| Journal | Histopathology |
| Volume | 21 |
| Issue number | 1 |
| Publication status | Published - Jul 1992 |
Keywords
- Antigens, Differentiation, B-Lymphocyte
- Base Sequence
- DNA, Viral
- Herpesvirus 4, Human
- Hodgkin Disease
- Humans
- Immunohistochemistry
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- Receptors, Complement
- Receptors, Complement 3d
- Receptors, Virus
- Reed-Sternberg Cells
- Tumor Virus Infections
- Journal Article