TY - JOUR
T1 - Epicardial application of cardiac progenitor cells in a 3D-printed gelatin/hyaluronic acid patch preserves cardiac function after myocardial infarction
AU - Gaetani, Roberto
AU - Feyen, Dries A M
AU - Verhage, Vera
AU - Slaats, Rolf
AU - Messina, Elisa
AU - Christman, Karen L.
AU - Giacomello, Alessandro
AU - Doevendans, Pieter A F M
AU - Sluijter, Joost P G
PY - 2015/8
Y1 - 2015/8
N2 - Cardiac cell therapy suffers from limitations related to poor engraftment and significant cell death after transplantation. In this regard, ex vivo tissue engineering is a tool that has been demonstrated to increase cell retention and survival. The aim of our study was to evaluate the therapeutic potential of a 3D-printed patch composed of human cardiac-derived progenitor cells (hCMPCs) in a hyaluronic acid/gelatin (HA/gel) based matrix. hCMPCs were printed in the HA/gel matrix (30 x 10(6) cells/ml) to form a biocomplex made of six perpendicularly printed layers with a surface of 2 x 2 cm and thickness of 400 um, in which they retained their viability, proliferation and differentiation capability. The printed biocomplex was transplanted in a mouse model of myocardial infarction (MI). The application of the patch led to a significant reduction in adverse remodeling and preservation of cardiac performance as was shown by both MRI and histology. Furthermore, the matrix supported the long-term in vivo survival and engraftment of hCMPCs, which exhibited a temporal increase in cardiac and vascular differentiation markers over the course of the 4 week follow-up period. Overall, we developed an effective and translational approach to enhance hCMPC delivery and action in the heart. (C) 2015 Elsevier Ltd. All rights reserved.
AB - Cardiac cell therapy suffers from limitations related to poor engraftment and significant cell death after transplantation. In this regard, ex vivo tissue engineering is a tool that has been demonstrated to increase cell retention and survival. The aim of our study was to evaluate the therapeutic potential of a 3D-printed patch composed of human cardiac-derived progenitor cells (hCMPCs) in a hyaluronic acid/gelatin (HA/gel) based matrix. hCMPCs were printed in the HA/gel matrix (30 x 10(6) cells/ml) to form a biocomplex made of six perpendicularly printed layers with a surface of 2 x 2 cm and thickness of 400 um, in which they retained their viability, proliferation and differentiation capability. The printed biocomplex was transplanted in a mouse model of myocardial infarction (MI). The application of the patch led to a significant reduction in adverse remodeling and preservation of cardiac performance as was shown by both MRI and histology. Furthermore, the matrix supported the long-term in vivo survival and engraftment of hCMPCs, which exhibited a temporal increase in cardiac and vascular differentiation markers over the course of the 4 week follow-up period. Overall, we developed an effective and translational approach to enhance hCMPC delivery and action in the heart. (C) 2015 Elsevier Ltd. All rights reserved.
KW - Cardiac progenitor cells
KW - Cardiac regeneration
KW - Cardiac tissue engineering
KW - Heart failure
KW - Tissue printing
UR - http://www.scopus.com/inward/record.url?scp=84939161281&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2015.05.005
DO - 10.1016/j.biomaterials.2015.05.005
M3 - Article
SN - 0142-9612
VL - 61
SP - 339
EP - 348
JO - Biomaterials
JF - Biomaterials
ER -