Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site

L. A. Vonk; T. S. de Windt; A. H M Kragten; M. Beekhuizen; S. C. Mastbergen; W. J A Dhert; F. P J G Lafeber; L. B. Creemers; D. B F Saris

doi:10.1016/j.joca.2014.08.005

Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site

L. A. Vonk^*
, T. S. de Windt
, A. H M Kragten
, M. Beekhuizen
, S. C. Mastbergen
, W. J A Dhert
, F. P J G Lafeber
, L. B. Creemers
, D. B F Saris

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

Objective: Interactions between chondrocytes and their native pericellular matrix provide optimal circumstances for regeneration of cartilage. However, cartilage diseases such as osteoarthritis change the pericellular matrix, causing doubt to them as a cell source for autologous cell therapy. Methods: Chondrons and chondrocytes were isolated from stifle joints of goats in which cartilage damage was surgically induced in the right knee. After 4 weeks of regeneration culture, DNA content and proteoglycan and collagen content and release were determined. Results: The cartilage regenerated by chondrons isolated from the damaged joint contained less proteoglycans and collagen compared to chondrons from the same harvest site in the nonoperated knee (P

Original language	English
Pages (from-to)	1910-1917
Number of pages	8
Journal	Osteoarthritis and Cartilage
Volume	22
Issue number	11
DOIs	https://doi.org/10.1016/j.joca.2014.08.005
Publication status	Published - 1 Nov 2014

Keywords

Autologous chondrocyte implantation
Cartilage repair
Cell therapy
Chondrocytes
Chondrons

Access to Document

10.1016/j.joca.2014.08.005

Cite this

Vonk, L. A., de Windt, T. S., Kragten, A. H. M., Beekhuizen, M., Mastbergen, S. C., Dhert, W. J. A., Lafeber, F. P. J. G., Creemers, L. B., & Saris, D. B. F. (2014). Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site. Osteoarthritis and Cartilage, 22(11), 1910-1917. https://doi.org/10.1016/j.joca.2014.08.005

@article{80919ba158234eb3b4f40b4791c4f201,

title = "Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site",

abstract = "Objective: Interactions between chondrocytes and their native pericellular matrix provide optimal circumstances for regeneration of cartilage. However, cartilage diseases such as osteoarthritis change the pericellular matrix, causing doubt to them as a cell source for autologous cell therapy. Methods: Chondrons and chondrocytes were isolated from stifle joints of goats in which cartilage damage was surgically induced in the right knee. After 4 weeks of regeneration culture, DNA content and proteoglycan and collagen content and release were determined. Results: The cartilage regenerated by chondrons isolated from the damaged joint contained less proteoglycans and collagen compared to chondrons from the same harvest site in the nonoperated knee (P",

keywords = "Autologous chondrocyte implantation, Cartilage repair, Cell therapy, Chondrocytes, Chondrons",

author = "Vonk, \{L. A.\} and \{de Windt\}, \{T. S.\} and Kragten, \{A. H M\} and M. Beekhuizen and Mastbergen, \{S. C.\} and Dhert, \{W. J A\} and Lafeber, \{F. P J G\} and Creemers, \{L. B.\} and Saris, \{D. B F\}",

year = "2014",

month = nov,

day = "1",

doi = "10.1016/j.joca.2014.08.005",

language = "English",

volume = "22",

pages = "1910--1917",

journal = "Osteoarthritis and Cartilage",

issn = "1063-4584",

publisher = "W.B. Saunders Ltd",

number = "11",

}

TY - JOUR

T1 - Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site

AU - Vonk, L. A.

AU - de Windt, T. S.

AU - Kragten, A. H M

AU - Beekhuizen, M.

AU - Mastbergen, S. C.

AU - Dhert, W. J A

AU - Lafeber, F. P J G

AU - Creemers, L. B.

AU - Saris, D. B F

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Objective: Interactions between chondrocytes and their native pericellular matrix provide optimal circumstances for regeneration of cartilage. However, cartilage diseases such as osteoarthritis change the pericellular matrix, causing doubt to them as a cell source for autologous cell therapy. Methods: Chondrons and chondrocytes were isolated from stifle joints of goats in which cartilage damage was surgically induced in the right knee. After 4 weeks of regeneration culture, DNA content and proteoglycan and collagen content and release were determined. Results: The cartilage regenerated by chondrons isolated from the damaged joint contained less proteoglycans and collagen compared to chondrons from the same harvest site in the nonoperated knee (P

AB - Objective: Interactions between chondrocytes and their native pericellular matrix provide optimal circumstances for regeneration of cartilage. However, cartilage diseases such as osteoarthritis change the pericellular matrix, causing doubt to them as a cell source for autologous cell therapy. Methods: Chondrons and chondrocytes were isolated from stifle joints of goats in which cartilage damage was surgically induced in the right knee. After 4 weeks of regeneration culture, DNA content and proteoglycan and collagen content and release were determined. Results: The cartilage regenerated by chondrons isolated from the damaged joint contained less proteoglycans and collagen compared to chondrons from the same harvest site in the nonoperated knee (P

KW - Autologous chondrocyte implantation

KW - Cartilage repair

KW - Cell therapy

KW - Chondrocytes

KW - Chondrons

UR - https://www.scopus.com/pages/publications/84908246557

U2 - 10.1016/j.joca.2014.08.005

DO - 10.1016/j.joca.2014.08.005

M3 - Article

C2 - 25151084

SN - 1063-4584

VL - 22

SP - 1910

EP - 1917

JO - Osteoarthritis and Cartilage

JF - Osteoarthritis and Cartilage

IS - 11

ER -

Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this