Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site

L. A. Vonk; T. S. de Windt; A. H M Kragten; M. Beekhuizen; S. C. Mastbergen; W. J A Dhert; F. P J G Lafeber; L. B. Creemers; D. B F Saris

doi:10.1016/j.joca.2014.08.005

Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site

L. A. Vonk^*, T. S. de Windt, A. H M Kragten, M. Beekhuizen, S. C. Mastbergen, W. J A Dhert, F. P J G Lafeber, L. B. Creemers, D. B F Saris

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

3 Citations (Scopus)

Abstract

Objective: Interactions between chondrocytes and their native pericellular matrix provide optimal circumstances for regeneration of cartilage. However, cartilage diseases such as osteoarthritis change the pericellular matrix, causing doubt to them as a cell source for autologous cell therapy. Methods: Chondrons and chondrocytes were isolated from stifle joints of goats in which cartilage damage was surgically induced in the right knee. After 4 weeks of regeneration culture, DNA content and proteoglycan and collagen content and release were determined. Results: The cartilage regenerated by chondrons isolated from the damaged joint contained less proteoglycans and collagen compared to chondrons from the same harvest site in the nonoperated knee (P

Original language	English
Pages (from-to)	1910-1917
Number of pages	8
Journal	Osteoarthritis and Cartilage
Volume	22
Issue number	11
DOIs	https://doi.org/10.1016/j.joca.2014.08.005
Publication status	Published - 1 Nov 2014

Keywords

Autologous chondrocyte implantation
Cartilage repair
Cell therapy
Chondrocytes
Chondrons

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Vonk, L. A., de Windt, T. S., Kragten, A. H. M., Beekhuizen, M., Mastbergen, S. C., Dhert, W. J. A., Lafeber, F. P. J. G., Creemers, L. B., & Saris, D. B. F. (2014). Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site. Osteoarthritis and Cartilage, 22(11), 1910-1917. https://doi.org/10.1016/j.joca.2014.08.005

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abstract = "Objective: Interactions between chondrocytes and their native pericellular matrix provide optimal circumstances for regeneration of cartilage. However, cartilage diseases such as osteoarthritis change the pericellular matrix, causing doubt to them as a cell source for autologous cell therapy. Methods: Chondrons and chondrocytes were isolated from stifle joints of goats in which cartilage damage was surgically induced in the right knee. After 4 weeks of regeneration culture, DNA content and proteoglycan and collagen content and release were determined. Results: The cartilage regenerated by chondrons isolated from the damaged joint contained less proteoglycans and collagen compared to chondrons from the same harvest site in the nonoperated knee (P",

keywords = "Autologous chondrocyte implantation, Cartilage repair, Cell therapy, Chondrocytes, Chondrons",

author = "Vonk, {L. A.} and {de Windt}, {T. S.} and Kragten, {A. H M} and M. Beekhuizen and Mastbergen, {S. C.} and Dhert, {W. J A} and Lafeber, {F. P J G} and Creemers, {L. B.} and Saris, {D. B F}",

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AU - Vonk, L. A.

AU - de Windt, T. S.

AU - Kragten, A. H M

AU - Beekhuizen, M.

AU - Mastbergen, S. C.

AU - Dhert, W. J A

AU - Lafeber, F. P J G

AU - Creemers, L. B.

AU - Saris, D. B F

PY - 2014/11/1

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N2 - Objective: Interactions between chondrocytes and their native pericellular matrix provide optimal circumstances for regeneration of cartilage. However, cartilage diseases such as osteoarthritis change the pericellular matrix, causing doubt to them as a cell source for autologous cell therapy. Methods: Chondrons and chondrocytes were isolated from stifle joints of goats in which cartilage damage was surgically induced in the right knee. After 4 weeks of regeneration culture, DNA content and proteoglycan and collagen content and release were determined. Results: The cartilage regenerated by chondrons isolated from the damaged joint contained less proteoglycans and collagen compared to chondrons from the same harvest site in the nonoperated knee (P

AB - Objective: Interactions between chondrocytes and their native pericellular matrix provide optimal circumstances for regeneration of cartilage. However, cartilage diseases such as osteoarthritis change the pericellular matrix, causing doubt to them as a cell source for autologous cell therapy. Methods: Chondrons and chondrocytes were isolated from stifle joints of goats in which cartilage damage was surgically induced in the right knee. After 4 weeks of regeneration culture, DNA content and proteoglycan and collagen content and release were determined. Results: The cartilage regenerated by chondrons isolated from the damaged joint contained less proteoglycans and collagen compared to chondrons from the same harvest site in the nonoperated knee (P

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KW - Cartilage repair

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KW - Chondrocytes

KW - Chondrons

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Enhanced cell-induced articular cartilage regeneration by chondrons; the influence of joint damage and harvest site

Abstract

Keywords

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