Enhanced cardiomyogenesis of human embryonic stem cells by a small molecular inhibitor of p38 MAPK

Translated title of the contribution: Enhanced cardiomyogenesis of human embryonic stem cells by a small molecular inhibitor of p38 MAPK

R. Graichen, X.Q. Xu, S.R. Braam, T. Balakrishnan, S. Norfiza, S. Sieh, S.Y. Soo, S.C. Tham, C.L. Mummery, A. Colman, R. Zweigerdt, B.P. Davidson

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    Human embryonic stem cells (hESC) can differentiate to cardiomyocytes in vitro but with generally poor efficiency. Here, we describe a novel method for the efficient generation of cardiomyocytes from hESC in a scalable suspension culture process. Differentiation in serum-free medium conditioned by the cell line END2 (END2-CM) readily resulted in differentiated cell populations with more than 10% cardiomyocytes without further enrichment. By screening candidate molecules, we have identified SB203580, a specific p38 MAP kinase inhibitor, as a potent promoter of hESC-cardiogenesis. SB203580 at concentrations = 15 mu M. Thus, modulation of the p38MAP kinase pathway, in combination with factors released by END2 cells, plays an essential role in early lineage determination in hESC and the efficiency of cardiomyogenesis. Our findings contribute to transforming human cardiomyocyte generation from hESC into a robust and scalable process
    Translated title of the contributionEnhanced cardiomyogenesis of human embryonic stem cells by a small molecular inhibitor of p38 MAPK
    Original languageUndefined/Unknown
    Pages (from-to)357-370
    Number of pages14
    JournalDifferentiation; Research in Biological Diversity
    Volume76
    Issue number4
    Publication statusPublished - 2008

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