ELISA-based detection of immunoglobulins against extracellular vesicles in blood plasma

Tom A P Driedonks; Linglei Jiang; Olesia Gololobova; Zhaohao Liao; Kenneth W Witwer

doi:10.1002/jex2.129

ELISA-based detection of immunoglobulins against extracellular vesicles in blood plasma

Tom A P Driedonks, Linglei Jiang, Olesia Gololobova, Zhaohao Liao, Kenneth W Witwer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Extracellular vesicles (EVs) are intensively investigated for their therapeutic potential and application as drug delivery vehicle. A broad perception of favourable safety profiles and low immunogenicity make EVs an attractive alternative to synthetic nanoparticles. We recently showed that repeated intravenous administration of human cell-derived EVs into pig-tailed macaques unexpectedly elicited antibody responses after three or more injections. This coincided with decreasing EV circulation time, and may thus hamper successful EV-mediated cargo delivery into tissues. Here, we share the custom ELISA protocol that we used to measure such antibody responses. This protocol may help other researchers evaluate immune responses to EV-based therapies in preclinical studies.

Original language	English
Article number	e129
Journal	Journal of Extracellular Biology
Volume	3
Issue number	3
DOIs	https://doi.org/10.1002/jex2.129
Publication status	Published - 7 Mar 2024
Externally published	Yes

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title = "ELISA-based detection of immunoglobulins against extracellular vesicles in blood plasma",

abstract = "Extracellular vesicles (EVs) are intensively investigated for their therapeutic potential and application as drug delivery vehicle. A broad perception of favourable safety profiles and low immunogenicity make EVs an attractive alternative to synthetic nanoparticles. We recently showed that repeated intravenous administration of human cell-derived EVs into pig-tailed macaques unexpectedly elicited antibody responses after three or more injections. This coincided with decreasing EV circulation time, and may thus hamper successful EV-mediated cargo delivery into tissues. Here, we share the custom ELISA protocol that we used to measure such antibody responses. This protocol may help other researchers evaluate immune responses to EV-based therapies in preclinical studies.",

author = "Driedonks, {Tom A P} and Linglei Jiang and Olesia Gololobova and Zhaohao Liao and Witwer, {Kenneth W}",

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AU - Driedonks, Tom A P

AU - Jiang, Linglei

AU - Gololobova, Olesia

AU - Liao, Zhaohao

AU - Witwer, Kenneth W

PY - 2024/3/7

Y1 - 2024/3/7

N2 - Extracellular vesicles (EVs) are intensively investigated for their therapeutic potential and application as drug delivery vehicle. A broad perception of favourable safety profiles and low immunogenicity make EVs an attractive alternative to synthetic nanoparticles. We recently showed that repeated intravenous administration of human cell-derived EVs into pig-tailed macaques unexpectedly elicited antibody responses after three or more injections. This coincided with decreasing EV circulation time, and may thus hamper successful EV-mediated cargo delivery into tissues. Here, we share the custom ELISA protocol that we used to measure such antibody responses. This protocol may help other researchers evaluate immune responses to EV-based therapies in preclinical studies.

AB - Extracellular vesicles (EVs) are intensively investigated for their therapeutic potential and application as drug delivery vehicle. A broad perception of favourable safety profiles and low immunogenicity make EVs an attractive alternative to synthetic nanoparticles. We recently showed that repeated intravenous administration of human cell-derived EVs into pig-tailed macaques unexpectedly elicited antibody responses after three or more injections. This coincided with decreasing EV circulation time, and may thus hamper successful EV-mediated cargo delivery into tissues. Here, we share the custom ELISA protocol that we used to measure such antibody responses. This protocol may help other researchers evaluate immune responses to EV-based therapies in preclinical studies.

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DO - 10.1002/jex2.129

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ER -

ELISA-based detection of immunoglobulins against extracellular vesicles in blood plasma

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