ELISA-based detection of immunoglobulins against extracellular vesicles in blood plasma

Tom A P Driedonks, Linglei Jiang, Olesia Gololobova, Zhaohao Liao, Kenneth W Witwer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Extracellular vesicles (EVs) are intensively investigated for their therapeutic potential and application as drug delivery vehicle. A broad perception of favourable safety profiles and low immunogenicity make EVs an attractive alternative to synthetic nanoparticles. We recently showed that repeated intravenous administration of human cell-derived EVs into pig-tailed macaques unexpectedly elicited antibody responses after three or more injections. This coincided with decreasing EV circulation time, and may thus hamper successful EV-mediated cargo delivery into tissues. Here, we share the custom ELISA protocol that we used to measure such antibody responses. This protocol may help other researchers evaluate immune responses to EV-based therapies in preclinical studies.

Original languageEnglish
Article numbere129
JournalJournal of Extracellular Biology
Issue number3
Publication statusPublished - 7 Mar 2024
Externally publishedYes


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