Elevated endothelial progenitor cells during painful sickle cell crisis

Rachel T. van Beem; Erfan Nur; Jaap Jan Zwaginga; Precious P. Landburg; Eduard J. van Beers; Ashley J. Duits; Dees P. Brandjes; Ingrid Lommerse; Hetty C. de Boer; C. Ellen van der Schoot; John-John B. Schnog; Bart J. Biemond

doi:10.1016/j.exphem.2009.06.003

Elevated endothelial progenitor cells during painful sickle cell crisis

Rachel T. van Beem, Erfan Nur, Jaap Jan Zwaginga, Precious P. Landburg, Eduard J. van Beers, Ashley J. Duits, Dees P. Brandjes, Ingrid Lommerse, Hetty C. de Boer, C. Ellen van der Schoot, John-John B. Schnog, Bart J. Biemond^*,

^*Corresponding author for this work

Hematology/Van Creveldclinic

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Objective. Circulating endothelial progenitor cells (EPCs) counts were determined in patients with sickle cell disease (SCD) to elucidate their role in SCD-related ischemia-induced angiogenesis and reendothelialization.

Materials and Methods. Circulating EPC counts (KDR(+)/CD34(+)/Cd45(dim) cells) and their relation to serum levels of EPC mobilizing growth factors erythropoietin, vascular endothelial growth factor, and interieukin-8 were investigated in SCD patients during asymptomatic state (n = 66) and painful crisis (n = 36) and compared to healthy controls (n = 13).

Results. EPC counts were comparable between controls (0; range, 0-1.1 cells/mL) and patients (0; range, 0-0 cells/mL) in asymptomatic state, but were significantly higher during painful crisis (41.7; range, 0-186 cells/mL; p <0.05). Also in a paired analysis of 12 patients who were included both during asymptomatic state and painful crisis, EPC counts increased significantly during painful crisis (from 0 [range, 0-0] to 26 [range, 0-149 cell/mL; p <0.05). EPC counts were not related to any of the measured growth factors.

Conclusion. The higher EPC counts during painful crisis might indicate a role for EPC mobilization in reendothelialization. As a relationship of EPCs with the established mobilizing growth factors, measured in this study was not observed, the mechanism of EPC mobilization in SCD remains to be elucidated. (C) 2009 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.

Original language	English
Pages (from-to)	1054-1059
Number of pages	6
Journal	Experimental Hematology
Volume	37
Issue number	9
DOIs	https://doi.org/10.1016/j.exphem.2009.06.003
Publication status	Published - Sept 2009

Keywords

NITRIC-OXIDE
CEREBROVASCULAR-DISEASE
ANGIOGENIC FACTORS
ERYTHROPOIETIN
MOBILIZATION
CONTRIBUTES
RETINOPATHY
HYPEROXIA
ADULTS
STATE

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10.1016/j.exphem.2009.06.003

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@article{083e4f8a8d7d40259fd33aad1ff93ede,

title = "Elevated endothelial progenitor cells during painful sickle cell crisis",

abstract = "Objective. Circulating endothelial progenitor cells (EPCs) counts were determined in patients with sickle cell disease (SCD) to elucidate their role in SCD-related ischemia-induced angiogenesis and reendothelialization.Materials and Methods. Circulating EPC counts (KDR(+)/CD34(+)/Cd45(dim) cells) and their relation to serum levels of EPC mobilizing growth factors erythropoietin, vascular endothelial growth factor, and interieukin-8 were investigated in SCD patients during asymptomatic state (n = 66) and painful crisis (n = 36) and compared to healthy controls (n = 13).Results. EPC counts were comparable between controls (0; range, 0-1.1 cells/mL) and patients (0; range, 0-0 cells/mL) in asymptomatic state, but were significantly higher during painful crisis (41.7; range, 0-186 cells/mL; p <0.05). Also in a paired analysis of 12 patients who were included both during asymptomatic state and painful crisis, EPC counts increased significantly during painful crisis (from 0 [range, 0-0] to 26 [range, 0-149 cell/mL; p <0.05). EPC counts were not related to any of the measured growth factors.Conclusion. The higher EPC counts during painful crisis might indicate a role for EPC mobilization in reendothelialization. As a relationship of EPCs with the established mobilizing growth factors, measured in this study was not observed, the mechanism of EPC mobilization in SCD remains to be elucidated. (C) 2009 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.",

keywords = "NITRIC-OXIDE, CEREBROVASCULAR-DISEASE, ANGIOGENIC FACTORS, ERYTHROPOIETIN, MOBILIZATION, CONTRIBUTES, RETINOPATHY, HYPEROXIA, ADULTS, STATE",

author = "{van Beem}, {Rachel T.} and Erfan Nur and Zwaginga, {Jaap Jan} and Landburg, {Precious P.} and {van Beers}, {Eduard J.} and Duits, {Ashley J.} and Brandjes, {Dees P.} and Ingrid Lommerse and {de Boer}, {Hetty C.} and {van der Schoot}, {C. Ellen} and Schnog, {John-John B.} and Biemond, {Bart J.}",

year = "2009",

month = sep,

doi = "10.1016/j.exphem.2009.06.003",

language = "English",

volume = "37",

pages = "1054--1059",

journal = "Experimental Hematology",

issn = "0301-472X",

publisher = "Elsevier",

number = "9",

}

TY - JOUR

T1 - Elevated endothelial progenitor cells during painful sickle cell crisis

AU - van Beem, Rachel T.

AU - Nur, Erfan

AU - Zwaginga, Jaap Jan

AU - Landburg, Precious P.

AU - van Beers, Eduard J.

AU - Duits, Ashley J.

AU - Brandjes, Dees P.

AU - Lommerse, Ingrid

AU - de Boer, Hetty C.

AU - van der Schoot, C. Ellen

AU - Schnog, John-John B.

AU - Biemond, Bart J.

PY - 2009/9

Y1 - 2009/9

N2 - Objective. Circulating endothelial progenitor cells (EPCs) counts were determined in patients with sickle cell disease (SCD) to elucidate their role in SCD-related ischemia-induced angiogenesis and reendothelialization.Materials and Methods. Circulating EPC counts (KDR(+)/CD34(+)/Cd45(dim) cells) and their relation to serum levels of EPC mobilizing growth factors erythropoietin, vascular endothelial growth factor, and interieukin-8 were investigated in SCD patients during asymptomatic state (n = 66) and painful crisis (n = 36) and compared to healthy controls (n = 13).Results. EPC counts were comparable between controls (0; range, 0-1.1 cells/mL) and patients (0; range, 0-0 cells/mL) in asymptomatic state, but were significantly higher during painful crisis (41.7; range, 0-186 cells/mL; p <0.05). Also in a paired analysis of 12 patients who were included both during asymptomatic state and painful crisis, EPC counts increased significantly during painful crisis (from 0 [range, 0-0] to 26 [range, 0-149 cell/mL; p <0.05). EPC counts were not related to any of the measured growth factors.Conclusion. The higher EPC counts during painful crisis might indicate a role for EPC mobilization in reendothelialization. As a relationship of EPCs with the established mobilizing growth factors, measured in this study was not observed, the mechanism of EPC mobilization in SCD remains to be elucidated. (C) 2009 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.

AB - Objective. Circulating endothelial progenitor cells (EPCs) counts were determined in patients with sickle cell disease (SCD) to elucidate their role in SCD-related ischemia-induced angiogenesis and reendothelialization.Materials and Methods. Circulating EPC counts (KDR(+)/CD34(+)/Cd45(dim) cells) and their relation to serum levels of EPC mobilizing growth factors erythropoietin, vascular endothelial growth factor, and interieukin-8 were investigated in SCD patients during asymptomatic state (n = 66) and painful crisis (n = 36) and compared to healthy controls (n = 13).Results. EPC counts were comparable between controls (0; range, 0-1.1 cells/mL) and patients (0; range, 0-0 cells/mL) in asymptomatic state, but were significantly higher during painful crisis (41.7; range, 0-186 cells/mL; p <0.05). Also in a paired analysis of 12 patients who were included both during asymptomatic state and painful crisis, EPC counts increased significantly during painful crisis (from 0 [range, 0-0] to 26 [range, 0-149 cell/mL; p <0.05). EPC counts were not related to any of the measured growth factors.Conclusion. The higher EPC counts during painful crisis might indicate a role for EPC mobilization in reendothelialization. As a relationship of EPCs with the established mobilizing growth factors, measured in this study was not observed, the mechanism of EPC mobilization in SCD remains to be elucidated. (C) 2009 ISEH - Society for Hematology and Stem Cells. Published by Elsevier Inc.

KW - NITRIC-OXIDE

KW - CEREBROVASCULAR-DISEASE

KW - ANGIOGENIC FACTORS

KW - ERYTHROPOIETIN

KW - MOBILIZATION

KW - CONTRIBUTES

KW - RETINOPATHY

KW - HYPEROXIA

KW - ADULTS

KW - STATE

U2 - 10.1016/j.exphem.2009.06.003

DO - 10.1016/j.exphem.2009.06.003

M3 - Article

C2 - 19539689

SN - 0301-472X

VL - 37

SP - 1054

EP - 1059

JO - Experimental Hematology

JF - Experimental Hematology

IS - 9

ER -

Elevated endothelial progenitor cells during painful sickle cell crisis

Abstract

Keywords

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