TY - JOUR
T1 - Electrospinning Lysine-Polypeptide Copolymers
T2 - Creating Microfiber Meshes for Biomedical Applications
AU - Salas-Ambrosio, Pedro
AU - Morales-Patlan, Enikar
AU - Cedillo-Servin, Gerardo
AU - Tronnet, Antoine
AU - Villavicencio, Katia Pamela
AU - Gómez-Lizárraga, Karla
AU - Benítez-Martínez, Jorge A.
AU - Sanchez-Arevalo, Francisco Manuel
AU - Velasquillo, Cristina
AU - Ceapă, Corina Diana
AU - Vera-Graziano, Ricardo
AU - Bonduelle, Colin
N1 - Publisher Copyright:
© 2024 The Authors. Published by American Chemical Society.
PY - 2024/8/9
Y1 - 2024/8/9
N2 - Polylysine is a synthetic polymer used in biology to promote cell proliferation. However, due to its high solubility in water, its incorporation into stable substrates is difficult to achieve using processes that enable 3D materials. In this work, we prepared electrospun fibers with high lysine content (greater than 90%) using high-molar-mass amphiphilic polypeptides (molar mass higher than 50 kDa) prepared by ring-opening copolymerization of N-carboxyanhydrides. By correctly selecting the fiber-forming conditions, it was possible to form homogeneous electrospun meshes with good mechanical properties (Young’s modulus from 100 to 500 MPa), allowing enhanced fibroblast cell proliferation (400% compared to the control) and exhibiting the characteristic fusiform morphology of adherent fibroblasts when cultured on the scaffolds, while avoiding polymer leaching from the fibers. This platform holds great promise for future biomedical applications, such as tissue engineering and wound healing.
AB - Polylysine is a synthetic polymer used in biology to promote cell proliferation. However, due to its high solubility in water, its incorporation into stable substrates is difficult to achieve using processes that enable 3D materials. In this work, we prepared electrospun fibers with high lysine content (greater than 90%) using high-molar-mass amphiphilic polypeptides (molar mass higher than 50 kDa) prepared by ring-opening copolymerization of N-carboxyanhydrides. By correctly selecting the fiber-forming conditions, it was possible to form homogeneous electrospun meshes with good mechanical properties (Young’s modulus from 100 to 500 MPa), allowing enhanced fibroblast cell proliferation (400% compared to the control) and exhibiting the characteristic fusiform morphology of adherent fibroblasts when cultured on the scaffolds, while avoiding polymer leaching from the fibers. This platform holds great promise for future biomedical applications, such as tissue engineering and wound healing.
KW - cell-growth
KW - electrospinning
KW - N-carboxyanhydride (NCA)
KW - polylysine
KW - ring-opening polymerization (ROP)
UR - http://www.scopus.com/inward/record.url?scp=85200377002&partnerID=8YFLogxK
U2 - 10.1021/acsapm.4c00344
DO - 10.1021/acsapm.4c00344
M3 - Article
AN - SCOPUS:85200377002
SN - 2637-6105
VL - 6
SP - 8733
EP - 8744
JO - ACS Applied Polymer Materials
JF - ACS Applied Polymer Materials
IS - 15
ER -