DNA methylation differences in cortical grey and white matter in schizophrenia

Amber Berdenis Van Berlekom; Nina Notman; Marjolein A.M. Sneeboer; Gijsje J.L.J. Snijders; Lotte C. Houtepen; Danny M. Nispeling; Yujie He; Stella Dracheva; Elly M. Hol; René S. Kahn; Lot D. De Witte; Marco P. Boks

doi:10.2217/epi-2021-0077

DNA methylation differences in cortical grey and white matter in schizophrenia

Amber Berdenis Van Berlekom, Nina Notman, Marjolein A.M. Sneeboer, Gijsje J.L.J. Snijders, Lotte C. Houtepen, Danny M. Nispeling, Yujie He, Stella Dracheva, Elly M. Hol, René S. Kahn, Lot D. De Witte, Marco P. Boks^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Aim: Identify grey- and white-matter-specific DNA-methylation differences between schizophrenia (SCZ) patients and controls in postmortem brain cortical tissue. Materials & methods: Grey and white matter were separated from postmortem brain tissue of the superior temporal and medial frontal gyrus from SCZ (n = 10) and control (n = 11) cases. Genome-wide DNA-methylation analysis was performed using the Infinium EPIC Methylation Array (Illumina, CA, USA). Results: Four differentially methylated regions associated with SCZ status and tissue type (grey vs white matter) were identified within or near KLF9, SFXN1, SPRED2 and ALS2CL genes. Gene-expression analysis showed differential expression of KLF9 and SFXN1 in SCZ. Conclusion: Our data show distinct differences in DNA methylation between grey and white matter that are unique to SCZ, providing new leads to unravel the pathogenesis of SCZ.

Original language	English
Pages (from-to)	1157-1169
Number of pages	13
Journal	Epigenomics
Volume	13
Issue number	15
Early online date	29 Jul 2021
DOIs	https://doi.org/10.2217/epi-2021-0077
Publication status	Published - Aug 2021

Keywords

DNA-methylation
postmortem
schizophrenia

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@article{a39a0ba776fb40ce8b7039a814b7d522,

title = "DNA methylation differences in cortical grey and white matter in schizophrenia",

abstract = "Aim: Identify grey- and white-matter-specific DNA-methylation differences between schizophrenia (SCZ) patients and controls in postmortem brain cortical tissue. Materials & methods: Grey and white matter were separated from postmortem brain tissue of the superior temporal and medial frontal gyrus from SCZ (n = 10) and control (n = 11) cases. Genome-wide DNA-methylation analysis was performed using the Infinium EPIC Methylation Array (Illumina, CA, USA). Results: Four differentially methylated regions associated with SCZ status and tissue type (grey vs white matter) were identified within or near KLF9, SFXN1, SPRED2 and ALS2CL genes. Gene-expression analysis showed differential expression of KLF9 and SFXN1 in SCZ. Conclusion: Our data show distinct differences in DNA methylation between grey and white matter that are unique to SCZ, providing new leads to unravel the pathogenesis of SCZ.",

keywords = "DNA-methylation, postmortem, schizophrenia",

author = "{Berdenis Van Berlekom}, Amber and Nina Notman and Sneeboer, {Marjolein A.M.} and Snijders, {Gijsje J.L.J.} and Houtepen, {Lotte C.} and Nispeling, {Danny M.} and Yujie He and Stella Dracheva and Hol, {Elly M.} and Kahn, {Ren{\'e} S.} and {De Witte}, {Lot D.} and Boks, {Marco P.}",

note = "Publisher Copyright: {\textcopyright} 2021 ",

year = "2021",

month = aug,

doi = "10.2217/epi-2021-0077",

language = "English",

volume = "13",

pages = "1157--1169",

journal = "Epigenomics",

issn = "1750-192X",

publisher = "Future Medicine Ltd.",

number = "15",

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TY - JOUR

T1 - DNA methylation differences in cortical grey and white matter in schizophrenia

AU - Berdenis Van Berlekom, Amber

AU - Notman, Nina

AU - Sneeboer, Marjolein A.M.

AU - Snijders, Gijsje J.L.J.

AU - Houtepen, Lotte C.

AU - Nispeling, Danny M.

AU - He, Yujie

AU - Dracheva, Stella

AU - Hol, Elly M.

AU - Kahn, René S.

AU - De Witte, Lot D.

AU - Boks, Marco P.

PY - 2021/8

Y1 - 2021/8

N2 - Aim: Identify grey- and white-matter-specific DNA-methylation differences between schizophrenia (SCZ) patients and controls in postmortem brain cortical tissue. Materials & methods: Grey and white matter were separated from postmortem brain tissue of the superior temporal and medial frontal gyrus from SCZ (n = 10) and control (n = 11) cases. Genome-wide DNA-methylation analysis was performed using the Infinium EPIC Methylation Array (Illumina, CA, USA). Results: Four differentially methylated regions associated with SCZ status and tissue type (grey vs white matter) were identified within or near KLF9, SFXN1, SPRED2 and ALS2CL genes. Gene-expression analysis showed differential expression of KLF9 and SFXN1 in SCZ. Conclusion: Our data show distinct differences in DNA methylation between grey and white matter that are unique to SCZ, providing new leads to unravel the pathogenesis of SCZ.

AB - Aim: Identify grey- and white-matter-specific DNA-methylation differences between schizophrenia (SCZ) patients and controls in postmortem brain cortical tissue. Materials & methods: Grey and white matter were separated from postmortem brain tissue of the superior temporal and medial frontal gyrus from SCZ (n = 10) and control (n = 11) cases. Genome-wide DNA-methylation analysis was performed using the Infinium EPIC Methylation Array (Illumina, CA, USA). Results: Four differentially methylated regions associated with SCZ status and tissue type (grey vs white matter) were identified within or near KLF9, SFXN1, SPRED2 and ALS2CL genes. Gene-expression analysis showed differential expression of KLF9 and SFXN1 in SCZ. Conclusion: Our data show distinct differences in DNA methylation between grey and white matter that are unique to SCZ, providing new leads to unravel the pathogenesis of SCZ.

KW - DNA-methylation

KW - postmortem

KW - schizophrenia

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U2 - 10.2217/epi-2021-0077

DO - 10.2217/epi-2021-0077

M3 - Article

C2 - 34323598

SN - 1750-192X

VL - 13

SP - 1157

EP - 1169

JO - Epigenomics

JF - Epigenomics

IS - 15

ER -

DNA methylation differences in cortical grey and white matter in schizophrenia

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