Directed Differentiation of Murine and Human Small Intestinal Organoids Toward All Mature Lineages

A. Martinez-Silgado, J. Beumer, H. Clevers*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Intestinal organoids are three-dimensional structures derived from tissue-resident adult stem cells. These organoids recapitulate key aspects of epithelial biology and can be used to study homeostatic turnover of the corresponding tissue. Organoids can be enriched for the various mature lineages which allows studies of the respective differentiation processes and of the diverse cellular functions. Here we describe mechanisms of intestinal fate specification and how these can be exploited to drive mouse and human small intestinal organoids into each of the functionally mature lineages.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages107-122
Number of pages16
Volume2650
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2650
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Adult stem cell-derived organoids
  • Differentiation
  • Intestinal lineages
  • Organoids
  • Small intestine

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