Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry

Eefje de Jong; Arjan S de Jong; Nathalie Smidts-van den Berg; Rob J Rentenaar

doi:10.1016/j.diagmicrobio.2012.12.009

Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry

Eefje de Jong, Arjan S de Jong, Nathalie Smidts-van den Berg, Rob J Rentenaar

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Ninety-nine clinical isolates previously identified as Klebsiella oxytoca were evaluated using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Eight isolates were identified as Raoultella spp., being 5 Raoultella spp. and 3 K. oxytoca, by 16S rRNA sequencing. These isolates were correctly identified by applying the 10% differential rule for the MALDI-TOF MS score values. This approach might be useful to discriminate Raoultella species from K. oxytoca.

Original language	English
Pages (from-to)	431-433
Number of pages	3
Journal	Diagnostic Microbiology and Infectious Disease
Volume	75
Issue number	4
DOIs	https://doi.org/10.1016/j.diagmicrobio.2012.12.009
Publication status	Published - Apr 2013
Externally published	Yes

Keywords

Bacteriological Techniques/methods
DNA, Bacterial/chemistry
DNA, Ribosomal/chemistry
Enterobacteriaceae/chemistry
Humans
Molecular Sequence Data
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods

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10.1016/j.diagmicrobio.2012.12.009

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de Jong, E., de Jong, A. S., Smidts-van den Berg, N., & Rentenaar, R. J. (2013). Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Diagnostic Microbiology and Infectious Disease, 75(4), 431-433. https://doi.org/10.1016/j.diagmicrobio.2012.12.009

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title = "Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry",

abstract = "Ninety-nine clinical isolates previously identified as Klebsiella oxytoca were evaluated using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Eight isolates were identified as Raoultella spp., being 5 Raoultella spp. and 3 K. oxytoca, by 16S rRNA sequencing. These isolates were correctly identified by applying the 10% differential rule for the MALDI-TOF MS score values. This approach might be useful to discriminate Raoultella species from K. oxytoca.",

keywords = "Bacteriological Techniques/methods, DNA, Bacterial/chemistry, DNA, Ribosomal/chemistry, Enterobacteriaceae/chemistry, Humans, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods",

author = "{de Jong}, Eefje and {de Jong}, {Arjan S} and {Smidts-van den Berg}, Nathalie and Rentenaar, {Rob J}",

year = "2013",

month = apr,

doi = "10.1016/j.diagmicrobio.2012.12.009",

language = "English",

volume = "75",

pages = "431--433",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier",

number = "4",

}

de Jong, E, de Jong, AS, Smidts-van den Berg, N & Rentenaar, RJ 2013, 'Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry', Diagnostic Microbiology and Infectious Disease, vol. 75, no. 4, pp. 431-433. https://doi.org/10.1016/j.diagmicrobio.2012.12.009

Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. / de Jong, Eefje; de Jong, Arjan S; Smidts-van den Berg, Nathalie et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 75, No. 4, 04.2013, p. 431-433.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry

AU - de Jong, Eefje

AU - de Jong, Arjan S

AU - Smidts-van den Berg, Nathalie

AU - Rentenaar, Rob J

PY - 2013/4

Y1 - 2013/4

N2 - Ninety-nine clinical isolates previously identified as Klebsiella oxytoca were evaluated using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Eight isolates were identified as Raoultella spp., being 5 Raoultella spp. and 3 K. oxytoca, by 16S rRNA sequencing. These isolates were correctly identified by applying the 10% differential rule for the MALDI-TOF MS score values. This approach might be useful to discriminate Raoultella species from K. oxytoca.

AB - Ninety-nine clinical isolates previously identified as Klebsiella oxytoca were evaluated using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Eight isolates were identified as Raoultella spp., being 5 Raoultella spp. and 3 K. oxytoca, by 16S rRNA sequencing. These isolates were correctly identified by applying the 10% differential rule for the MALDI-TOF MS score values. This approach might be useful to discriminate Raoultella species from K. oxytoca.

KW - Bacteriological Techniques/methods

KW - DNA, Bacterial/chemistry

KW - DNA, Ribosomal/chemistry

KW - Enterobacteriaceae/chemistry

KW - Humans

KW - Molecular Sequence Data

KW - RNA, Ribosomal, 16S/genetics

KW - Sequence Analysis, DNA

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods

U2 - 10.1016/j.diagmicrobio.2012.12.009

DO - 10.1016/j.diagmicrobio.2012.12.009

M3 - Article

C2 - 23375086

SN - 0732-8893

VL - 75

SP - 431

EP - 433

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 4

ER -

Differentiation of Raoultella ornithinolytica/planticola and Klebsiella oxytoca clinical isolates by matrix-assisted laser desorption/ionization-time of flight mass spectrometry

Abstract

Keywords

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