Abstract
BACKGROUND: We have previously reported that human adenovirus (HAdV) reference strains clearly show species-dependent resistance to ribavirin, whereas different species of HAdV are equally sensitive to cidofovir. All the serotypes tested were susceptible to cidofovir, whereas only serotypes from species C were sensitive to ribavirin. Here, we aimed to extend these investigations to clinical isolates.
METHODS: In vitro, we tested 126 isolates obtained from 65 patients included in a European survey of HAdV infection.
RESULTS: Among the 126 isolates tested, all presented cidofovir 50% inhibitory concentration (IC50) in the same range as the HAdV 5 reference strain. Regarding ribavirin, all isolates from species C (79 tested) showed an IC50 comparable with previously reported results for reference strains; however, 24/32, 2/6 and 3/3 tested isolates from species A, B and D, respectively, were shown to have a ribavirin IC50 comparable with the HAdV 5 reference strain (species C), contrary to previous observations for reference strains of the same species. Among patients who were treated with cidofovir for disseminated HAdV infection, > or = 4 sequential isolates could be obtained from 9 patients; no variation in cidofovir susceptibility could be detected.
CONCLUSIONS: Cidofovir is active in vitro in all HAdV clinical isolates. Ribavirin was revealed to be active on most HAdV isolates from species A, B and D, and in all isolates from species C. Finally, no resistance to cidofovir became apparent in sequential isolates obtained from treated patients.
Original language | English |
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Pages (from-to) | 55-61 |
Number of pages | 7 |
Journal | Antiviral Therapy |
Volume | 14 |
Issue number | 1 |
Publication status | Published - 2009 |
Keywords
- Adenoviridae
- Adenovirus Infections, Human
- Antiviral Agents
- Cell Line, Tumor
- Cytosine
- DNA, Viral
- Drug Resistance, Viral
- Genotype
- Humans
- Organophosphonates
- Ribavirin
- Serotyping
- Species Specificity