TY - JOUR
T1 - Differential expression of TNFR1 (CD120a) and TNFR2 (CD120b) on subpopulations of human monocytes
AU - Hijdra, Daniëlle
AU - Vorselaars, Adriane Dm
AU - Grutters, Jan C.
AU - Claessen, Anke Me
AU - Rijkers, Ger T.
N1 - Funding Information:
This work was supported by the Innovation Fund of the St. Antonius Hospital in Nieuwegein, The Netherlands.
PY - 2012
Y1 - 2012
N2 - Background: Three subpopulations of monocytes can be distinguished in human blood: classical (CD14§ssup§++§esup§CD16§ssup§- §esup§), intermediate (CD14§ssup§++§esup§ CD16§ssup§+§esup§), and nonclassical (CD14§ssup§ +§esup§CD16§ssup§++§esup§). CD16 expressing monocytes are expanded in patients with sarcoidosis and in various other inflammatory diseases. In sarcoidosis, it is unclear whether either intermediate, nonclassical or both CD16 expressing monocytes are responsible for this increase. Data relating to the monocyte subpopulations is receiving increasing attention, but the expression of TNF receptors on these subpopulations has not been studied thus far. The aim of this study was to determine frequencies of monocyte subpopulations and their expression of TNFR1 and TNFR2 in both sarcoidosis patients and healthy controls. Methods. Peripheral blood cells of sarcoidosis patients and healthy controls were stained for the markers HLA-DR, CD14, CD16, CD120a and CD120b. Cells were measured on a FACSCalibur and analyzed with FlowJo. We used Student's t-test and a parametric One-way ANOVA for statistical analysis. Results: Sarcoidosis patients had a significant higher frequency of intermediate monocytes than healthy controls. Significant differences in TNF receptor expression were found between the monocyte subpopulations, both in sarcoidosis patients as well as in healthy controls: intermediates expressed more TNFR1 than classicals and nonclassicals and nonclassicals expressed more TNFR2 than intermediates, whereas intermediates showed higher expression than classicals. Conclusions: In both sarcoidosis patients and healthy controls intermediate monocytes show the highest expression level of TNFR1 among monocyte subpopulations and nonclassical monocytes show the highest expression level of TNFR2. These findings, as wells as the higher frequency of intermediate monocytes in sarcoidosis patients, provide evidence for the existence of two functionally-distinct CD16 expressing monocyte subpopulations.
AB - Background: Three subpopulations of monocytes can be distinguished in human blood: classical (CD14§ssup§++§esup§CD16§ssup§- §esup§), intermediate (CD14§ssup§++§esup§ CD16§ssup§+§esup§), and nonclassical (CD14§ssup§ +§esup§CD16§ssup§++§esup§). CD16 expressing monocytes are expanded in patients with sarcoidosis and in various other inflammatory diseases. In sarcoidosis, it is unclear whether either intermediate, nonclassical or both CD16 expressing monocytes are responsible for this increase. Data relating to the monocyte subpopulations is receiving increasing attention, but the expression of TNF receptors on these subpopulations has not been studied thus far. The aim of this study was to determine frequencies of monocyte subpopulations and their expression of TNFR1 and TNFR2 in both sarcoidosis patients and healthy controls. Methods. Peripheral blood cells of sarcoidosis patients and healthy controls were stained for the markers HLA-DR, CD14, CD16, CD120a and CD120b. Cells were measured on a FACSCalibur and analyzed with FlowJo. We used Student's t-test and a parametric One-way ANOVA for statistical analysis. Results: Sarcoidosis patients had a significant higher frequency of intermediate monocytes than healthy controls. Significant differences in TNF receptor expression were found between the monocyte subpopulations, both in sarcoidosis patients as well as in healthy controls: intermediates expressed more TNFR1 than classicals and nonclassicals and nonclassicals expressed more TNFR2 than intermediates, whereas intermediates showed higher expression than classicals. Conclusions: In both sarcoidosis patients and healthy controls intermediate monocytes show the highest expression level of TNFR1 among monocyte subpopulations and nonclassical monocytes show the highest expression level of TNFR2. These findings, as wells as the higher frequency of intermediate monocytes in sarcoidosis patients, provide evidence for the existence of two functionally-distinct CD16 expressing monocyte subpopulations.
KW - CD120a
KW - CD120b
KW - CD14
KW - CD16
KW - HLA-DR
KW - Monocytes
KW - Peripheral blood
KW - Sarcoidosis
KW - TNF receptor
UR - http://www.scopus.com/inward/record.url?scp=84867025709&partnerID=8YFLogxK
U2 - 10.1186/1476-9255-9-38
DO - 10.1186/1476-9255-9-38
M3 - Article
AN - SCOPUS:84867025709
VL - 9
JO - Journal of Inflammation (United Kingdom)
JF - Journal of Inflammation (United Kingdom)
M1 - 38
ER -