Differential expression of estrogen receptor mRNA and protein in the female rat preoptic area

R. Jeroen Pasterkamp; Kazunari Yuri; Noriyuki Morita; Mitsuhiro Kawata

doi:10.1016/S0304-3940(97)00888-4

Differential expression of estrogen receptor mRNA and protein in the female rat preoptic area

R. Jeroen Pasterkamp, Kazunari Yuri^*, Noriyuki Morita, Mitsuhiro Kawata

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

The expression of estrogen receptor (ER) mRNA and ER protein in the medial preoptic area of ovariectomized rat was investigated at both cellular and regional levels using non-isotopic in situ hybridization and immunohistochemistry. ER mRNA was localized in the cytoplasm while both liganded and unliganded forms of the ER protein were confined to the nucleus. Furthermore, ER mRNA containing cells were evenly distributed throughout the medial preoptic area, showing a homogeneous staining pattern compared to that of ER protein. ER immunoreactive cells were highly distributed in the medial, moderately in the lateral aspect of the medial preoptic area, showing a heterogeneous staining pattern with strongly and weakly labeled cells. These results suggest that ER protein levels are controlled by cellular posttranscriptional mechanisms.

Original language	English
Pages (from-to)	81-84
Number of pages	4
Journal	Neuroscience Letters
Volume	239
Issue number	2-3
DOIs	https://doi.org/10.1016/S0304-3940(97)00888-4
Publication status	Published - 15 Dec 1997

Keywords

Estrogen receptor
Immunohistochemistry
Medial preoptic area
Non-isotopic in situ hybridization
Ovariectomy

Access to Document

10.1016/S0304-3940(97)00888-4

Cite this

@article{aaed735980ce485e89fbc44876b41e71,

title = "Differential expression of estrogen receptor mRNA and protein in the female rat preoptic area",

abstract = "The expression of estrogen receptor (ER) mRNA and ER protein in the medial preoptic area of ovariectomized rat was investigated at both cellular and regional levels using non-isotopic in situ hybridization and immunohistochemistry. ER mRNA was localized in the cytoplasm while both liganded and unliganded forms of the ER protein were confined to the nucleus. Furthermore, ER mRNA containing cells were evenly distributed throughout the medial preoptic area, showing a homogeneous staining pattern compared to that of ER protein. ER immunoreactive cells were highly distributed in the medial, moderately in the lateral aspect of the medial preoptic area, showing a heterogeneous staining pattern with strongly and weakly labeled cells. These results suggest that ER protein levels are controlled by cellular posttranscriptional mechanisms.",

keywords = "Estrogen receptor, Immunohistochemistry, Medial preoptic area, Non-isotopic in situ hybridization, Ovariectomy",

author = "Pasterkamp, {R. Jeroen} and Kazunari Yuri and Noriyuki Morita and Mitsuhiro Kawata",

year = "1997",

month = dec,

day = "15",

doi = "10.1016/S0304-3940(97)00888-4",

language = "English",

volume = "239",

pages = "81--84",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier Ireland Ltd",

number = "2-3",

}

TY - JOUR

T1 - Differential expression of estrogen receptor mRNA and protein in the female rat preoptic area

AU - Pasterkamp, R. Jeroen

AU - Yuri, Kazunari

AU - Morita, Noriyuki

AU - Kawata, Mitsuhiro

PY - 1997/12/15

Y1 - 1997/12/15

N2 - The expression of estrogen receptor (ER) mRNA and ER protein in the medial preoptic area of ovariectomized rat was investigated at both cellular and regional levels using non-isotopic in situ hybridization and immunohistochemistry. ER mRNA was localized in the cytoplasm while both liganded and unliganded forms of the ER protein were confined to the nucleus. Furthermore, ER mRNA containing cells were evenly distributed throughout the medial preoptic area, showing a homogeneous staining pattern compared to that of ER protein. ER immunoreactive cells were highly distributed in the medial, moderately in the lateral aspect of the medial preoptic area, showing a heterogeneous staining pattern with strongly and weakly labeled cells. These results suggest that ER protein levels are controlled by cellular posttranscriptional mechanisms.

AB - The expression of estrogen receptor (ER) mRNA and ER protein in the medial preoptic area of ovariectomized rat was investigated at both cellular and regional levels using non-isotopic in situ hybridization and immunohistochemistry. ER mRNA was localized in the cytoplasm while both liganded and unliganded forms of the ER protein were confined to the nucleus. Furthermore, ER mRNA containing cells were evenly distributed throughout the medial preoptic area, showing a homogeneous staining pattern compared to that of ER protein. ER immunoreactive cells were highly distributed in the medial, moderately in the lateral aspect of the medial preoptic area, showing a heterogeneous staining pattern with strongly and weakly labeled cells. These results suggest that ER protein levels are controlled by cellular posttranscriptional mechanisms.

KW - Estrogen receptor

KW - Immunohistochemistry

KW - Medial preoptic area

KW - Non-isotopic in situ hybridization

KW - Ovariectomy

UR - http://www.scopus.com/inward/record.url?scp=0031578960&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(97)00888-4

DO - 10.1016/S0304-3940(97)00888-4

M3 - Article

C2 - 9469661

AN - SCOPUS:0031578960

SN - 0304-3940

VL - 239

SP - 81

EP - 84

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 2-3

ER -

Differential expression of estrogen receptor mRNA and protein in the female rat preoptic area

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this