Differential effects of ACTH-related peptides and forskolin on rat Schwann cell proliferation and low affinity NGF receptor expression

Elly M. Hol; Diane A. Houweling; Peter Sodaar; P. R.Dop Bär

doi:10.1002/(SICI)1520-6769(199607)19:1<37::AID-NRC161>3.0.CO;2-C

Differential effects of ACTH-related peptides and forskolin on rat Schwann cell proliferation and low affinity NGF receptor expression

Elly M. Hol^*, Diane A. Houweling, Peter Sodaar, P. R.Dop Bär

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We investigated the effect of the neurotrophic peptides αMSH and Org 2766 on Schwann cell proliferation (BrdU incorporation) and low affinity nerve growth factor receptor (LNGFR) expression in vitro. Cells were cultured under four conditions: low or high density culture, with forskolin present or absent in the medium during one week before start of the experiment. Schwann cells were treated with αMSH, Org 2766 or forskolin. After 48h forskolin (10 μM) increased BrdU incorporation (32-151%) in all culture conditions and LNGFR production (25-194%) in 3 out of 4 conditions. αMSH and Org 2766 had no effect. This study shows that Schwann cell proliferation and LNGFR expression is under control of cAMP. Furthermore, the neurotrophic effect of (αMSH and Org 2766 is probably not mediated through an increased cell division or LNGFR expression in Schwann cells.

Original language	English
Pages (from-to)	37-45
Number of pages	9
Journal	Neuroscience Research Communications
Volume	19
Issue number	1
DOIs	https://doi.org/10.1002/(SICI)1520-6769(199607)19:1<37::AID-NRC161>3.0.CO;2-C
Publication status	Published - 1 Jul 1996

Keywords

αMSH
ACTH-analogue
Melanocortins
Neurotrophic peptides
Schwann cells

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10.1002/(SICI)1520-6769(199607)19:1<37::AID-NRC161>3.0.CO;2-C

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title = "Differential effects of ACTH-related peptides and forskolin on rat Schwann cell proliferation and low affinity NGF receptor expression",

abstract = "We investigated the effect of the neurotrophic peptides αMSH and Org 2766 on Schwann cell proliferation (BrdU incorporation) and low affinity nerve growth factor receptor (LNGFR) expression in vitro. Cells were cultured under four conditions: low or high density culture, with forskolin present or absent in the medium during one week before start of the experiment. Schwann cells were treated with αMSH, Org 2766 or forskolin. After 48h forskolin (10 μM) increased BrdU incorporation (32-151%) in all culture conditions and LNGFR production (25-194%) in 3 out of 4 conditions. αMSH and Org 2766 had no effect. This study shows that Schwann cell proliferation and LNGFR expression is under control of cAMP. Furthermore, the neurotrophic effect of (αMSH and Org 2766 is probably not mediated through an increased cell division or LNGFR expression in Schwann cells.",

keywords = "αMSH, ACTH-analogue, Melanocortins, Neurotrophic peptides, Schwann cells",

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AU - Hol, Elly M.

AU - Houweling, Diane A.

AU - Sodaar, Peter

AU - Bär, P. R.Dop

PY - 1996/7/1

Y1 - 1996/7/1

N2 - We investigated the effect of the neurotrophic peptides αMSH and Org 2766 on Schwann cell proliferation (BrdU incorporation) and low affinity nerve growth factor receptor (LNGFR) expression in vitro. Cells were cultured under four conditions: low or high density culture, with forskolin present or absent in the medium during one week before start of the experiment. Schwann cells were treated with αMSH, Org 2766 or forskolin. After 48h forskolin (10 μM) increased BrdU incorporation (32-151%) in all culture conditions and LNGFR production (25-194%) in 3 out of 4 conditions. αMSH and Org 2766 had no effect. This study shows that Schwann cell proliferation and LNGFR expression is under control of cAMP. Furthermore, the neurotrophic effect of (αMSH and Org 2766 is probably not mediated through an increased cell division or LNGFR expression in Schwann cells.

AB - We investigated the effect of the neurotrophic peptides αMSH and Org 2766 on Schwann cell proliferation (BrdU incorporation) and low affinity nerve growth factor receptor (LNGFR) expression in vitro. Cells were cultured under four conditions: low or high density culture, with forskolin present or absent in the medium during one week before start of the experiment. Schwann cells were treated with αMSH, Org 2766 or forskolin. After 48h forskolin (10 μM) increased BrdU incorporation (32-151%) in all culture conditions and LNGFR production (25-194%) in 3 out of 4 conditions. αMSH and Org 2766 had no effect. This study shows that Schwann cell proliferation and LNGFR expression is under control of cAMP. Furthermore, the neurotrophic effect of (αMSH and Org 2766 is probably not mediated through an increased cell division or LNGFR expression in Schwann cells.

KW - αMSH

KW - ACTH-analogue

KW - Melanocortins

KW - Neurotrophic peptides

KW - Schwann cells

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JO - Neuroscience Research Communications

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Differential effects of ACTH-related peptides and forskolin on rat Schwann cell proliferation and low affinity NGF receptor expression

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Keywords

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