Dietary fish oil reduces the incidence of triggered arrhythmias in pig ventricular myocytes

Geza Berecki; Hester M. Den Ruijter; Arie O. Verkerk; Cees A. Schumacher; Antonius Baartscheer; Diane Bakker; Bastiaan J. Boukens; Antoni C. G. van Ginneken; Jan W. T. Fiolet; Tobias Opthof; Ruben Coronel

doi:10.1016/j.hrthm.2007.07.015

Dietary fish oil reduces the incidence of triggered arrhythmias in pig ventricular myocytes

Geza Berecki, Hester M. Den Ruijter, Arie O. Verkerk, Cees A. Schumacher, Antonius Baartscheer, Diane Bakker, Bastiaan J. Boukens, Antoni C. G. van Ginneken, Jan W. T. Fiolet, Tobias Opthof, Ruben Coronel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND Fish oil reduces the incidence of sudden cardiac death in postmyocardial infarction patients. Triggered activity is the principal mechanism of arrhythmogenesis under these conditions.

OBJECTIVE The purpose of this study was to test whether dietary fish oil in pigs inhibits Ca2+ overload-induced triggered activity.

METHODS Pigs were fed a diet of fish oil or sunflower oil for 8 weeks. Ventricular myocytes (omega 3: fish oil, n = 11; control: sunflower oil, n = 8) were isolated by enzymatic dissociation and used for patch clamp studies and intracellular Ca2+ recordings. Triggered activity was induced by rapid pacing in the presence of norepinephrine.

RESULTS Dietary fish oil reduced the incidence of triggered action potentials and delayed after depotarizations compared to control (9.1% in omega 3 and 84.6% in control, P

CONCLUSION Dietary fish oil reduces the incidence of triggered activity and prevents Ca2+ overload and AP prolongation in response to norepinephrine. Fish oil may prevent arrhythmias in patients with heart failure.

Original language	English
Pages (from-to)	1452-1460
Number of pages	9
Journal	Heart Rhythm
Volume	4
Issue number	11
DOIs	https://doi.org/10.1016/j.hrthm.2007.07.015
Publication status	Published - Nov 2007

Keywords

fish oil
omega 3-polyunsaturated fatty acid
triggered activity
delayed afterdepotarization
intracellular calcium
norepinephrine
arrhythmia
electrophysiology
POLYUNSATURATED FATTY-ACIDS
CA2+-ACTIVATED CL-CURRENT
OUTWARD CHLORIDE CURRENT
STAGE FAILING HEARTS
DELAYED AFTERDEPOLARIZATIONS
MYOCARDIAL-INFARCTION
ACTION-POTENTIALS
PURKINJE-FIBERS
CHANNEL
REPOLARIZATION

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10.1016/j.hrthm.2007.07.015

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@article{df176210dfae402bbc09fec4c3a995fe,

title = "Dietary fish oil reduces the incidence of triggered arrhythmias in pig ventricular myocytes",

abstract = "BACKGROUND Fish oil reduces the incidence of sudden cardiac death in postmyocardial infarction patients. Triggered activity is the principal mechanism of arrhythmogenesis under these conditions.OBJECTIVE The purpose of this study was to test whether dietary fish oil in pigs inhibits Ca2+ overload-induced triggered activity.METHODS Pigs were fed a diet of fish oil or sunflower oil for 8 weeks. Ventricular myocytes (omega 3: fish oil, n = 11; control: sunflower oil, n = 8) were isolated by enzymatic dissociation and used for patch clamp studies and intracellular Ca2+ recordings. Triggered activity was induced by rapid pacing in the presence of norepinephrine.RESULTS Dietary fish oil reduced the incidence of triggered action potentials and delayed after depotarizations compared to control (9.1% in omega 3 and 84.6% in control, PCONCLUSION Dietary fish oil reduces the incidence of triggered activity and prevents Ca2+ overload and AP prolongation in response to norepinephrine. Fish oil may prevent arrhythmias in patients with heart failure.",

keywords = "fish oil, omega 3-polyunsaturated fatty acid, triggered activity, delayed afterdepotarization, intracellular calcium, norepinephrine, arrhythmia, electrophysiology, POLYUNSATURATED FATTY-ACIDS, CA2+-ACTIVATED CL-CURRENT, OUTWARD CHLORIDE CURRENT, STAGE FAILING HEARTS, DELAYED AFTERDEPOLARIZATIONS, MYOCARDIAL-INFARCTION, ACTION-POTENTIALS, PURKINJE-FIBERS, CHANNEL, REPOLARIZATION",

author = "Geza Berecki and {Den Ruijter}, {Hester M.} and Verkerk, {Arie O.} and Schumacher, {Cees A.} and Antonius Baartscheer and Diane Bakker and Boukens, {Bastiaan J.} and {van Ginneken}, {Antoni C. G.} and Fiolet, {Jan W. T.} and Tobias Opthof and Ruben Coronel",

year = "2007",

month = nov,

doi = "10.1016/j.hrthm.2007.07.015",

language = "English",

volume = "4",

pages = "1452--1460",

journal = "Heart Rhythm",

issn = "1547-5271",

publisher = "Elsevier",

number = "11",

}

TY - JOUR

T1 - Dietary fish oil reduces the incidence of triggered arrhythmias in pig ventricular myocytes

AU - Berecki, Geza

AU - Den Ruijter, Hester M.

AU - Verkerk, Arie O.

AU - Schumacher, Cees A.

AU - Baartscheer, Antonius

AU - Bakker, Diane

AU - Boukens, Bastiaan J.

AU - van Ginneken, Antoni C. G.

AU - Fiolet, Jan W. T.

AU - Opthof, Tobias

AU - Coronel, Ruben

PY - 2007/11

Y1 - 2007/11

N2 - BACKGROUND Fish oil reduces the incidence of sudden cardiac death in postmyocardial infarction patients. Triggered activity is the principal mechanism of arrhythmogenesis under these conditions.OBJECTIVE The purpose of this study was to test whether dietary fish oil in pigs inhibits Ca2+ overload-induced triggered activity.METHODS Pigs were fed a diet of fish oil or sunflower oil for 8 weeks. Ventricular myocytes (omega 3: fish oil, n = 11; control: sunflower oil, n = 8) were isolated by enzymatic dissociation and used for patch clamp studies and intracellular Ca2+ recordings. Triggered activity was induced by rapid pacing in the presence of norepinephrine.RESULTS Dietary fish oil reduced the incidence of triggered action potentials and delayed after depotarizations compared to control (9.1% in omega 3 and 84.6% in control, PCONCLUSION Dietary fish oil reduces the incidence of triggered activity and prevents Ca2+ overload and AP prolongation in response to norepinephrine. Fish oil may prevent arrhythmias in patients with heart failure.

AB - BACKGROUND Fish oil reduces the incidence of sudden cardiac death in postmyocardial infarction patients. Triggered activity is the principal mechanism of arrhythmogenesis under these conditions.OBJECTIVE The purpose of this study was to test whether dietary fish oil in pigs inhibits Ca2+ overload-induced triggered activity.METHODS Pigs were fed a diet of fish oil or sunflower oil for 8 weeks. Ventricular myocytes (omega 3: fish oil, n = 11; control: sunflower oil, n = 8) were isolated by enzymatic dissociation and used for patch clamp studies and intracellular Ca2+ recordings. Triggered activity was induced by rapid pacing in the presence of norepinephrine.RESULTS Dietary fish oil reduced the incidence of triggered action potentials and delayed after depotarizations compared to control (9.1% in omega 3 and 84.6% in control, PCONCLUSION Dietary fish oil reduces the incidence of triggered activity and prevents Ca2+ overload and AP prolongation in response to norepinephrine. Fish oil may prevent arrhythmias in patients with heart failure.

KW - fish oil

KW - omega 3-polyunsaturated fatty acid

KW - triggered activity

KW - delayed afterdepotarization

KW - intracellular calcium

KW - norepinephrine

KW - arrhythmia

KW - electrophysiology

KW - POLYUNSATURATED FATTY-ACIDS

KW - CA2+-ACTIVATED CL-CURRENT

KW - OUTWARD CHLORIDE CURRENT

KW - STAGE FAILING HEARTS

KW - DELAYED AFTERDEPOLARIZATIONS

KW - MYOCARDIAL-INFARCTION

KW - ACTION-POTENTIALS

KW - PURKINJE-FIBERS

KW - CHANNEL

KW - REPOLARIZATION

U2 - 10.1016/j.hrthm.2007.07.015

DO - 10.1016/j.hrthm.2007.07.015

M3 - Article

C2 - 17954406

SN - 1547-5271

VL - 4

SP - 1452

EP - 1460

JO - Heart Rhythm

JF - Heart Rhythm

IS - 11

ER -

Dietary fish oil reduces the incidence of triggered arrhythmias in pig ventricular myocytes

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Keywords

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