Diagnostic Utility of Menin Immunohistochemistry in Patients With Multiple Endocrine Neoplasia Type 1 Syndrome

Anna Vera D Verschuur*, Aranxa S M Kok, Folkert H M Morsink, Wendy W J de Leng, Medard F M van den Broek, Marco J Koudijs, Johan A Offerhaus, Gerlof D Valk, Menno R Vriens, Bernadette P M van Nesselrooij, Wenzel M Hackeng, Lodewijk A A Brosens

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

A clinical diagnosis of multiple endocrine neoplasia type 1 (MEN1) syndrome is usually confirmed with genetic testing in the germline. It is expected that menin protein expression is lost in MEN1-related tumors. Therefore, we investigated the potential of menin immunohistochemistry in parathyroid adenomas as an additional tool in the recognition and genetic diagnosis of MEN1 syndrome. Local pathology archives were searched for parathyroid tumors from patients with MEN1 syndrome and without MEN1, including sporadic, patients with multiple endocrine neoplasia type 2A and hyperparathyroidism-jaw parathyroid tumors. Menin immunohistochemistry was performed and its use to identify MEN1-related tumors was assessed. Twenty-nine parathyroid tumors from 16 patients with MEN1 and 61 patients with parathyroid tumors from 32 non-MEN1 were evaluated. Immunohistochemical nuclear menin loss in one or more tumors was found in 100% of patients with MEN1 and 9% of patients with non-MEN1. In patients with multiple tumors, menin loss in at least one tumor was seen in 100% of 8 patients with MEN1 and 21% of patients with 14 non-MEN1. Using a cutoff of at least 2 tumors showing menin loss per patient, the positive and negative predictive values for the diagnosis MEN1 were both 100%. The practical and additional value of menin immunohistochemistry in clinical genetic MEN1 diagnosis is further illustrated by menin immunohistochemistry in 2 cases with a germline variant of unknown significance in the MEN1 gene. Menin immunohistochemistry is useful in the recognition of MEN1 syndrome as well as in the clinical genetic analysis of patients with inconclusive MEN1 germline testing.

Original languageEnglish
Pages (from-to)785-791
Number of pages7
JournalAmerican Journal of Surgical Pathology
Volume47
Issue number7
Early online date18 May 2023
DOIs
Publication statusPublished - 1 Jul 2023

Keywords

  • MEN1
  • adenoma
  • hyperplasia
  • immunohistochemistry
  • menin
  • multiple endocrine neoplasia type 1 syndrome
  • parathyroid

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