TY - JOUR
T1 - Diagnostic accuracy study of multiplex PCR for detecting tuberculosis drug resistance
AU - Molina-Moya, B.
AU - Lacoma, A.
AU - Prat, C.
AU - Pimkina, E.
AU - Diaz, J.
AU - García-Sierra, N.
AU - Haba, L.
AU - Maldonado, J.
AU - Samper, S.
AU - Ruiz-Manzano, J.
AU - Ausina, V.
AU - Dominguez, J.
N1 - Funding Information:
This work was supported by a grant from the Instituto de Salud Carlos III ( FIS PI 13/01546 ). Izasa supplied the quantity of Anyplex II MTB/MDR/XDR kits necessary for the study. No companies played a role in the study design, conduct, collection, management, analysis, or interpretation of the data or the preparation, review, or approval of the manuscript.
Publisher Copyright:
© 2015 The British Infection Association.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - Objective: To study the diagnostic accuracy of a multiplex real-time PCR (Anyplex II MTB/MDR/XDR, Seegene, Corea) that detects Mycobacterium tuberculosis resistant to isoniazid (INH), rifampicin (RIF), fluoroquinolones (FLQ) and injectable drugs (kanamycin [KAN], amikacin [AMK] and capreomycin [CAP]) in isolates and specimens. Methods: One hundred fourteen cultured isolates and 73 sputum specimens were retrospectively selected. Results obtained with multiplex PCR were compared with those obtained with BACTEC. Discordant results between multiplex PCR and BACTEC were tested by alternative molecular methods. Results: Sensitivity and specificity of multiplex PCR for detecting drug resistance in isolates were 76.5% and 100%, respectively, for INH; 97.2% and 96.0%, respectively, for RIF; 70.4% and 87.9%, respectively, for FLQ; 81.5% and 84.8%, respectively, for KAN; 100% and 60%, respectively, for AMK, and 100% and 72.3%, respectively, for CAP. Sensitivity and specificity of Anyplex for detecting drug resistance in specimens were 93.3% and 100%, respectively, for INH; 100% and 100%, respectively, for RIF; 50.0% and 100%, respectively, for FLQ; and 100% and 94.4%, respectively, for both KAN and CAP. Among the discordant results, 87.7% (71/81) of results obtained with the multiplex PCR were concordant with at least one of the alternative molecular methods. Conclusions: This multiplex PCR may be a useful tool for the rapid identification of drug resistant tuberculosis in isolates and specimens, thus allowing an initial therapeutic approach. Nevertheless, for a correct management of patients, results should be confirmed by a phenotypic method.
AB - Objective: To study the diagnostic accuracy of a multiplex real-time PCR (Anyplex II MTB/MDR/XDR, Seegene, Corea) that detects Mycobacterium tuberculosis resistant to isoniazid (INH), rifampicin (RIF), fluoroquinolones (FLQ) and injectable drugs (kanamycin [KAN], amikacin [AMK] and capreomycin [CAP]) in isolates and specimens. Methods: One hundred fourteen cultured isolates and 73 sputum specimens were retrospectively selected. Results obtained with multiplex PCR were compared with those obtained with BACTEC. Discordant results between multiplex PCR and BACTEC were tested by alternative molecular methods. Results: Sensitivity and specificity of multiplex PCR for detecting drug resistance in isolates were 76.5% and 100%, respectively, for INH; 97.2% and 96.0%, respectively, for RIF; 70.4% and 87.9%, respectively, for FLQ; 81.5% and 84.8%, respectively, for KAN; 100% and 60%, respectively, for AMK, and 100% and 72.3%, respectively, for CAP. Sensitivity and specificity of Anyplex for detecting drug resistance in specimens were 93.3% and 100%, respectively, for INH; 100% and 100%, respectively, for RIF; 50.0% and 100%, respectively, for FLQ; and 100% and 94.4%, respectively, for both KAN and CAP. Among the discordant results, 87.7% (71/81) of results obtained with the multiplex PCR were concordant with at least one of the alternative molecular methods. Conclusions: This multiplex PCR may be a useful tool for the rapid identification of drug resistant tuberculosis in isolates and specimens, thus allowing an initial therapeutic approach. Nevertheless, for a correct management of patients, results should be confirmed by a phenotypic method.
KW - Fluoroquinolones
KW - Isoniazid
KW - Molecular resistance detection
KW - Multiplex real-time PCR
KW - Rifampicin
KW - Second-line injectable drugs
KW - Tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=84937516929&partnerID=8YFLogxK
U2 - 10.1016/j.jinf.2015.03.011
DO - 10.1016/j.jinf.2015.03.011
M3 - Article
C2 - 25936742
AN - SCOPUS:84937516929
SN - 0163-4453
VL - 71
SP - 220
EP - 230
JO - Journal of Infection
JF - Journal of Infection
IS - 2
ER -