TY - JOUR
T1 - Development of the Nasopharyngeal Microbiota in Infants with Cystic Fibrosis
AU - Prevaes, Sabine M P J
AU - de Winter-de Groot, Karin M
AU - Janssens, Hettie M
AU - de Steenhuijsen Piters, Wouter A A
AU - Tramper-Stranders, Gerdien A
AU - Wyllie, Anne L
AU - Hasrat, Raiza
AU - Tiddens, Harm A
AU - van Westreenen, Mireille
AU - van der Ent, Cornelis K.
AU - Sanders, Elisabeth A M
AU - Bogaert, Debby
PY - 2015
Y1 - 2015
N2 - RATIONALE: Cystic Fibrosis (CF) is characterized by early structural lung disease caused by pulmonary infections. The nasopharynx of infants is a major ecological reservoir of potential respiratory pathogens.OBJECTIVE: To investigate the development of nasopharyngeal microbiota profiles in infants with CF compared with those of healthy controls during the first 6 months of life.METHODS: We conducted a prospective cohort study, collecting questionnaires and 324 nasopharynx samples of 20 CF infants and 45 age-matched healthy controls from diagnosis. Microbiota profiles were characterized by 16S-rRNA-based sequencing.MAIN RESULTS: We observed significant differences in microbial community composition (PERMANOVA, p<0.0002) and development between groups; in infants with CF, early Staphylococcus aureus and to a lesser extent, Corynebacterium spp. and Moraxella spp. dominance was followed by a switch to Streptococcus mitis predominance after 3 months of age, versus Moraxella spp. enrichment throughout the first 6 months of life in controls. In a multivariate analysis S. aureus, S. mitis, Corynebacterium accolens and bacilli. were significantly more abundant in CF whereas Moraxella spp., Corynebacterium pseudodiphtericum/propinquum and Haemophilus influenzae were significantly more abundant in controls, after correction for age, antibiotic use, and respiratory symptoms. Antibiotic use was independently associated with increased colonization of gram-negative bacteria such as Burkholderia and Enterobacteriacae spp. and reduced colonization of potential beneficial commensals.CONCLUSION: From diagnosis onwards, we observed distinct patterns of nasopharyngeal microbiota development in infants with CF under 6 months of age versus controls and a marked effect of antibiotic therapy towards a gram-negative microbial composition.
AB - RATIONALE: Cystic Fibrosis (CF) is characterized by early structural lung disease caused by pulmonary infections. The nasopharynx of infants is a major ecological reservoir of potential respiratory pathogens.OBJECTIVE: To investigate the development of nasopharyngeal microbiota profiles in infants with CF compared with those of healthy controls during the first 6 months of life.METHODS: We conducted a prospective cohort study, collecting questionnaires and 324 nasopharynx samples of 20 CF infants and 45 age-matched healthy controls from diagnosis. Microbiota profiles were characterized by 16S-rRNA-based sequencing.MAIN RESULTS: We observed significant differences in microbial community composition (PERMANOVA, p<0.0002) and development between groups; in infants with CF, early Staphylococcus aureus and to a lesser extent, Corynebacterium spp. and Moraxella spp. dominance was followed by a switch to Streptococcus mitis predominance after 3 months of age, versus Moraxella spp. enrichment throughout the first 6 months of life in controls. In a multivariate analysis S. aureus, S. mitis, Corynebacterium accolens and bacilli. were significantly more abundant in CF whereas Moraxella spp., Corynebacterium pseudodiphtericum/propinquum and Haemophilus influenzae were significantly more abundant in controls, after correction for age, antibiotic use, and respiratory symptoms. Antibiotic use was independently associated with increased colonization of gram-negative bacteria such as Burkholderia and Enterobacteriacae spp. and reduced colonization of potential beneficial commensals.CONCLUSION: From diagnosis onwards, we observed distinct patterns of nasopharyngeal microbiota development in infants with CF under 6 months of age versus controls and a marked effect of antibiotic therapy towards a gram-negative microbial composition.
U2 - 10.1164/rccm.201509-1759OC
DO - 10.1164/rccm.201509-1759OC
M3 - Article
C2 - 26492486
SN - 1073-449X
JO - American Journal of Respiratory and Critical Care Medicine
JF - American Journal of Respiratory and Critical Care Medicine
ER -