Development of a simple passive haemagglutination-inhibition assay for Listeria monocytogenes lipoteichoic acid

AD C J M Antonissen; Kok P M Van Kessel; Hans Van Dijk; Jan M N Willers

doi:10.1016/0022-1759(81)90053-3

Development of a simple passive haemagglutination-inhibition assay for Listeria monocytogenes lipoteichoic acid

AD C J M Antonissen^*, Kok P M Van Kessel, Hans Van Dijk, Jan M N Willers

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A simple assay for listeria lipoteichoic acid was devised. It is based on the property of this substance to attach spontaneously to erythrocytes. Thus coated rabbit erythrocytes were agglutinated by a rabbit antiserum raised towards dead listeria. Inhibition of this passive agglutination was used as a measure of listeria lipoteichoic acid. Cross-reactivity of lipoteichoic acid from Lactobacillus fermenti in the assay and inhibition by simple sugars suggest that the assay is specific for terminal galactose.

Original language	English
Pages (from-to)	351-357
Number of pages	7
Journal	Journal of Immunological Methods
Volume	44
Issue number	3
DOIs	https://doi.org/10.1016/0022-1759(81)90053-3
Publication status	Published - 14 Aug 1981

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10.1016/0022-1759(81)90053-3

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abstract = "A simple assay for listeria lipoteichoic acid was devised. It is based on the property of this substance to attach spontaneously to erythrocytes. Thus coated rabbit erythrocytes were agglutinated by a rabbit antiserum raised towards dead listeria. Inhibition of this passive agglutination was used as a measure of listeria lipoteichoic acid. Cross-reactivity of lipoteichoic acid from Lactobacillus fermenti in the assay and inhibition by simple sugars suggest that the assay is specific for terminal galactose.",

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AU - Willers, Jan M N

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AB - A simple assay for listeria lipoteichoic acid was devised. It is based on the property of this substance to attach spontaneously to erythrocytes. Thus coated rabbit erythrocytes were agglutinated by a rabbit antiserum raised towards dead listeria. Inhibition of this passive agglutination was used as a measure of listeria lipoteichoic acid. Cross-reactivity of lipoteichoic acid from Lactobacillus fermenti in the assay and inhibition by simple sugars suggest that the assay is specific for terminal galactose.

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Development of a simple passive haemagglutination-inhibition assay for Listeria monocytogenes lipoteichoic acid

Abstract

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