Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing

Jessy V van Asperen; Emma J van Bodegraven; Pierre A J T Robe; Elly M Hol

doi:10.1016/j.xpro.2022.101703

Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing

Jessy V van Asperen
, Emma J van Bodegraven
, Pierre A J T Robe
, Elly M Hol

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The ex vivo organotypic brain slice invasion model is commonly used to study the growth dynamics of gliomas, primary brain tumors that are known for their invasive behavior. Here, we describe a protocol where the ex vivo organotypic mouse brain slice invasion model is combined with whole-mount immunostaining, tissue clearing, and 3D reconstruction, to visualize and quantify the invasion of glioma cells. In addition, we describe an approach to determine the proliferation rate of the cells within this model. For complete details on the use and execution of this protocol, please refer to Uceda-Castro et al. (2022).

Original language	English
Article number	101703
Journal	STAR protocols
Volume	3
Issue number	4
Early online date	21 Sept 2022
DOIs	https://doi.org/10.1016/j.xpro.2022.101703
Publication status	Published - 16 Dec 2022

Keywords

Antibody
Cancer
Cell biology
Microscopy
Neuroscience

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10.1016/j.xpro.2022.101703Licence: CC BY

1-s2.0-S2666166722005834-mainFinal published version, 5.55 MBLicence: CC BY

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title = "Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing",

abstract = "The ex vivo organotypic brain slice invasion model is commonly used to study the growth dynamics of gliomas, primary brain tumors that are known for their invasive behavior. Here, we describe a protocol where the ex vivo organotypic mouse brain slice invasion model is combined with whole-mount immunostaining, tissue clearing, and 3D reconstruction, to visualize and quantify the invasion of glioma cells. In addition, we describe an approach to determine the proliferation rate of the cells within this model. For complete details on the use and execution of this protocol, please refer to Uceda-Castro et al. (2022).",

keywords = "Antibody, Cancer, Cell biology, Microscopy, Neuroscience",

author = "\{van Asperen\}, \{Jessy V\} and \{van Bodegraven\}, \{Emma J\} and Robe, \{Pierre A J T\} and Hol, \{Elly M\}",

note = "Funding Information: We thank Youri Adolfs for the help with the whole-mount immunostaining protocol and Imaris Software analysis, and Lo{\"i}s Aitatus and Nicky Kronenburg for the help with making the photographs and video. This study was funded by the Dutch Cancer Society (KWF 101123 ) and the T and P Bohnenn Foundation (P.R.). Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

month = dec,

day = "16",

doi = "10.1016/j.xpro.2022.101703",

language = "English",

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T1 - Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing

AU - van Asperen, Jessy V

AU - van Bodegraven, Emma J

AU - Robe, Pierre A J T

AU - Hol, Elly M

N1 - Funding Information: We thank Youri Adolfs for the help with the whole-mount immunostaining protocol and Imaris Software analysis, and Loïs Aitatus and Nicky Kronenburg for the help with making the photographs and video. This study was funded by the Dutch Cancer Society (KWF 101123 ) and the T and P Bohnenn Foundation (P.R.). Publisher Copyright: © 2022 The Author(s)

PY - 2022/12/16

Y1 - 2022/12/16

N2 - The ex vivo organotypic brain slice invasion model is commonly used to study the growth dynamics of gliomas, primary brain tumors that are known for their invasive behavior. Here, we describe a protocol where the ex vivo organotypic mouse brain slice invasion model is combined with whole-mount immunostaining, tissue clearing, and 3D reconstruction, to visualize and quantify the invasion of glioma cells. In addition, we describe an approach to determine the proliferation rate of the cells within this model. For complete details on the use and execution of this protocol, please refer to Uceda-Castro et al. (2022).

AB - The ex vivo organotypic brain slice invasion model is commonly used to study the growth dynamics of gliomas, primary brain tumors that are known for their invasive behavior. Here, we describe a protocol where the ex vivo organotypic mouse brain slice invasion model is combined with whole-mount immunostaining, tissue clearing, and 3D reconstruction, to visualize and quantify the invasion of glioma cells. In addition, we describe an approach to determine the proliferation rate of the cells within this model. For complete details on the use and execution of this protocol, please refer to Uceda-Castro et al. (2022).

KW - Antibody

KW - Cancer

KW - Cell biology

KW - Microscopy

KW - Neuroscience

UR - https://www.scopus.com/pages/publications/85138179256

U2 - 10.1016/j.xpro.2022.101703

DO - 10.1016/j.xpro.2022.101703

M3 - Article

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SN - 2666-1667

VL - 3

JO - STAR protocols

JF - STAR protocols

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M1 - 101703

ER -

Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing

Abstract

Keywords

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