TY - JOUR
T1 - Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing
AU - van Asperen, Jessy V
AU - van Bodegraven, Emma J
AU - Robe, Pierre A J T
AU - Hol, Elly M
N1 - Funding Information:
We thank Youri Adolfs for the help with the whole-mount immunostaining protocol and Imaris Software analysis, and Loïs Aitatus and Nicky Kronenburg for the help with making the photographs and video. This study was funded by the Dutch Cancer Society (KWF 101123 ) and the T and P Bohnenn Foundation (P.R.).
Publisher Copyright:
© 2022 The Author(s)
PY - 2022/12/16
Y1 - 2022/12/16
N2 - The ex vivo organotypic brain slice invasion model is commonly used to study the growth dynamics of gliomas, primary brain tumors that are known for their invasive behavior. Here, we describe a protocol where the ex vivo organotypic mouse brain slice invasion model is combined with whole-mount immunostaining, tissue clearing, and 3D reconstruction, to visualize and quantify the invasion of glioma cells. In addition, we describe an approach to determine the proliferation rate of the cells within this model. For complete details on the use and execution of this protocol, please refer to Uceda-Castro et al. (2022).
AB - The ex vivo organotypic brain slice invasion model is commonly used to study the growth dynamics of gliomas, primary brain tumors that are known for their invasive behavior. Here, we describe a protocol where the ex vivo organotypic mouse brain slice invasion model is combined with whole-mount immunostaining, tissue clearing, and 3D reconstruction, to visualize and quantify the invasion of glioma cells. In addition, we describe an approach to determine the proliferation rate of the cells within this model. For complete details on the use and execution of this protocol, please refer to Uceda-Castro et al. (2022).
KW - Antibody
KW - Cancer
KW - Cell biology
KW - Microscopy
KW - Neuroscience
UR - http://www.scopus.com/inward/record.url?scp=85138179256&partnerID=8YFLogxK
U2 - 10.1016/j.xpro.2022.101703
DO - 10.1016/j.xpro.2022.101703
M3 - Article
C2 - 36136755
SN - 2666-1667
VL - 3
JO - STAR protocols
JF - STAR protocols
IS - 4
M1 - 101703
ER -