Determination of proteinase 3-α1-antitrypsin complexes in inflammatory fluids

Koert M. Dolman; Bart A. van de Wiel; Chih Min Kam; Jannie J. Abbink; C. Erik Hack; Arnoud Sonnenberg; James C. Powers; Albert E.G.Kr von dem Borne; Roel Goldschmeding

doi:10.1016/0014-5793(92)80955-G

Determination of proteinase 3-α₁-antitrypsin complexes in inflammatory fluids

Koert M. Dolman
, Bart A. van de Wiel
, Chih Min Kam
, Jannie J. Abbink
, C. Erik Hack
, Arnoud Sonnenberg
, James C. Powers
, Albert E.G.Kr von dem Borne
, Roel Goldschmeding^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

24 Citations (Scopus)

Abstract

Physiological inhibitors were tested for their in vitro interaction with neutrophil proteinase 3 (PR3). The major plasma proteinase inhibitor of PR3 is α₁AT. We have developed a radioimmunoassay (RIA) for quantitative detection of PR3-α₁AT complexes formed in vivo in inflammatory exudates such as synovial fluid and plasma from patients with sepsis. Levels of PR3-α₁AT complexes correlated significantly with levels of human neutrophil elastase (HNE)-α₁AT complexes. Thus, in vivo α₁AT not only protects against excessive HNE activity, but also against excessive PR3 activity.

Original language	English
Pages (from-to)	117-121
Number of pages	5
Journal	FEBS letters
Volume	314
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(92)80955-G
Publication status	Published - 14 Dec 1992
Externally published	Yes

Keywords

Inflammatory fluid
Proteinase 3
α-Antitrypsin

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10.1016/0014-5793(92)80955-G

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@article{e33498a4850d437694cd690703bb6d86,

title = "Determination of proteinase 3-α1-antitrypsin complexes in inflammatory fluids",

abstract = "Physiological inhibitors were tested for their in vitro interaction with neutrophil proteinase 3 (PR3). The major plasma proteinase inhibitor of PR3 is α1AT. We have developed a radioimmunoassay (RIA) for quantitative detection of PR3-α1AT complexes formed in vivo in inflammatory exudates such as synovial fluid and plasma from patients with sepsis. Levels of PR3-α1AT complexes correlated significantly with levels of human neutrophil elastase (HNE)-α1AT complexes. Thus, in vivo α1AT not only protects against excessive HNE activity, but also against excessive PR3 activity.",

keywords = "Inflammatory fluid, Proteinase 3, α-Antitrypsin",

author = "Dolman, \{Koert M.\} and \{van de Wiel\}, \{Bart A.\} and Kam, \{Chih Min\} and Abbink, \{Jannie J.\} and Hack, \{C. Erik\} and Arnoud Sonnenberg and Powers, \{James C.\} and \{von dem Borne\}, \{Albert E.G.Kr\} and Roel Goldschmeding",

note = "Funding Information: and Dr. L.G. Thij\textasciitilde{} foi providing patient\textasciitilde{} material. This research wa\textasciitilde{} supported by a grant from The Dutch Kidney Foundation (C88.733), and partially supported by a grant to the Georgm Institute of Teehnol-og), (GM 42212) from the National lnhtitute\textasciitilde{} of Health.",

year = "1992",

month = dec,

day = "14",

doi = "10.1016/0014-5793(92)80955-G",

language = "English",

volume = "314",

pages = "117--121",

journal = "FEBS letters",

issn = "0014-5793",

publisher = "John Wiley \& Sons Inc.",

number = "2",

}

TY - JOUR

T1 - Determination of proteinase 3-α1-antitrypsin complexes in inflammatory fluids

AU - Dolman, Koert M.

AU - van de Wiel, Bart A.

AU - Kam, Chih Min

AU - Abbink, Jannie J.

AU - Hack, C. Erik

AU - Sonnenberg, Arnoud

AU - Powers, James C.

AU - von dem Borne, Albert E.G.Kr

AU - Goldschmeding, Roel

N1 - Funding Information: and Dr. L.G. Thij~ foi providing patient~ material. This research wa~ supported by a grant from The Dutch Kidney Foundation (C88.733), and partially supported by a grant to the Georgm Institute of Teehnol-og), (GM 42212) from the National lnhtitute~ of Health.

PY - 1992/12/14

Y1 - 1992/12/14

N2 - Physiological inhibitors were tested for their in vitro interaction with neutrophil proteinase 3 (PR3). The major plasma proteinase inhibitor of PR3 is α1AT. We have developed a radioimmunoassay (RIA) for quantitative detection of PR3-α1AT complexes formed in vivo in inflammatory exudates such as synovial fluid and plasma from patients with sepsis. Levels of PR3-α1AT complexes correlated significantly with levels of human neutrophil elastase (HNE)-α1AT complexes. Thus, in vivo α1AT not only protects against excessive HNE activity, but also against excessive PR3 activity.

AB - Physiological inhibitors were tested for their in vitro interaction with neutrophil proteinase 3 (PR3). The major plasma proteinase inhibitor of PR3 is α1AT. We have developed a radioimmunoassay (RIA) for quantitative detection of PR3-α1AT complexes formed in vivo in inflammatory exudates such as synovial fluid and plasma from patients with sepsis. Levels of PR3-α1AT complexes correlated significantly with levels of human neutrophil elastase (HNE)-α1AT complexes. Thus, in vivo α1AT not only protects against excessive HNE activity, but also against excessive PR3 activity.

KW - Inflammatory fluid

KW - Proteinase 3

KW - α-Antitrypsin

UR - https://www.scopus.com/pages/publications/0026458638

U2 - 10.1016/0014-5793(92)80955-G

DO - 10.1016/0014-5793(92)80955-G

M3 - Article

C2 - 1459241

AN - SCOPUS:0026458638

SN - 0014-5793

VL - 314

SP - 117

EP - 121

JO - FEBS letters

JF - FEBS letters

IS - 2

ER -

Determination of proteinase 3-α₁-antitrypsin complexes in inflammatory fluids

Abstract

Keywords

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