Determination of proteinase 3-α1-antitrypsin complexes in inflammatory fluids

Koert M. Dolman; Bart A. van de Wiel; Chih Min Kam; Jannie J. Abbink; C. Erik Hack; Arnoud Sonnenberg; James C. Powers; Albert E.G.Kr von dem Borne; Roel Goldschmeding

doi:10.1016/0014-5793(92)80955-G

Determination of proteinase 3-α₁-antitrypsin complexes in inflammatory fluids

Koert M. Dolman, Bart A. van de Wiel, Chih Min Kam, Jannie J. Abbink, C. Erik Hack, Arnoud Sonnenberg, James C. Powers, Albert E.G.Kr von dem Borne, Roel Goldschmeding^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

24 Citations (Scopus)

Abstract

Physiological inhibitors were tested for their in vitro interaction with neutrophil proteinase 3 (PR3). The major plasma proteinase inhibitor of PR3 is α₁AT. We have developed a radioimmunoassay (RIA) for quantitative detection of PR3-α₁AT complexes formed in vivo in inflammatory exudates such as synovial fluid and plasma from patients with sepsis. Levels of PR3-α₁AT complexes correlated significantly with levels of human neutrophil elastase (HNE)-α₁AT complexes. Thus, in vivo α₁AT not only protects against excessive HNE activity, but also against excessive PR3 activity.

Original language	English
Pages (from-to)	117-121
Number of pages	5
Journal	FEBS letters
Volume	314
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(92)80955-G
Publication status	Published - 14 Dec 1992
Externally published	Yes

Keywords

Inflammatory fluid
Proteinase 3
α-Antitrypsin

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10.1016/0014-5793(92)80955-G

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@article{e33498a4850d437694cd690703bb6d86,

title = "Determination of proteinase 3-α1-antitrypsin complexes in inflammatory fluids",

abstract = "Physiological inhibitors were tested for their in vitro interaction with neutrophil proteinase 3 (PR3). The major plasma proteinase inhibitor of PR3 is α1AT. We have developed a radioimmunoassay (RIA) for quantitative detection of PR3-α1AT complexes formed in vivo in inflammatory exudates such as synovial fluid and plasma from patients with sepsis. Levels of PR3-α1AT complexes correlated significantly with levels of human neutrophil elastase (HNE)-α1AT complexes. Thus, in vivo α1AT not only protects against excessive HNE activity, but also against excessive PR3 activity.",

keywords = "Inflammatory fluid, Proteinase 3, α-Antitrypsin",

author = "Dolman, {Koert M.} and {van de Wiel}, {Bart A.} and Kam, {Chih Min} and Abbink, {Jannie J.} and Hack, {C. Erik} and Arnoud Sonnenberg and Powers, {James C.} and {von dem Borne}, {Albert E.G.Kr} and Roel Goldschmeding",

note = "Funding Information: and Dr. L.G. Thij~ foi providing patient~ material. This research wa~ supported by a grant from The Dutch Kidney Foundation (C88.733), and partially supported by a grant to the Georgm Institute of Teehnol-og), (GM 42212) from the National lnhtitute~ of Health.",

year = "1992",

month = dec,

day = "14",

doi = "10.1016/0014-5793(92)80955-G",

language = "English",

volume = "314",

pages = "117--121",

journal = "FEBS letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Determination of proteinase 3-α1-antitrypsin complexes in inflammatory fluids

AU - Dolman, Koert M.

AU - van de Wiel, Bart A.

AU - Kam, Chih Min

AU - Abbink, Jannie J.

AU - Hack, C. Erik

AU - Sonnenberg, Arnoud

AU - Powers, James C.

AU - von dem Borne, Albert E.G.Kr

AU - Goldschmeding, Roel

N1 - Funding Information: and Dr. L.G. Thij~ foi providing patient~ material. This research wa~ supported by a grant from The Dutch Kidney Foundation (C88.733), and partially supported by a grant to the Georgm Institute of Teehnol-og), (GM 42212) from the National lnhtitute~ of Health.

PY - 1992/12/14

Y1 - 1992/12/14

N2 - Physiological inhibitors were tested for their in vitro interaction with neutrophil proteinase 3 (PR3). The major plasma proteinase inhibitor of PR3 is α1AT. We have developed a radioimmunoassay (RIA) for quantitative detection of PR3-α1AT complexes formed in vivo in inflammatory exudates such as synovial fluid and plasma from patients with sepsis. Levels of PR3-α1AT complexes correlated significantly with levels of human neutrophil elastase (HNE)-α1AT complexes. Thus, in vivo α1AT not only protects against excessive HNE activity, but also against excessive PR3 activity.

AB - Physiological inhibitors were tested for their in vitro interaction with neutrophil proteinase 3 (PR3). The major plasma proteinase inhibitor of PR3 is α1AT. We have developed a radioimmunoassay (RIA) for quantitative detection of PR3-α1AT complexes formed in vivo in inflammatory exudates such as synovial fluid and plasma from patients with sepsis. Levels of PR3-α1AT complexes correlated significantly with levels of human neutrophil elastase (HNE)-α1AT complexes. Thus, in vivo α1AT not only protects against excessive HNE activity, but also against excessive PR3 activity.

KW - Inflammatory fluid

KW - Proteinase 3

KW - α-Antitrypsin

UR - http://www.scopus.com/inward/record.url?scp=0026458638&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(92)80955-G

DO - 10.1016/0014-5793(92)80955-G

M3 - Article

C2 - 1459241

AN - SCOPUS:0026458638

SN - 0014-5793

VL - 314

SP - 117

EP - 121

JO - FEBS letters

JF - FEBS letters

IS - 2

ER -

Determination of proteinase 3-α₁-antitrypsin complexes in inflammatory fluids

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Keywords

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