Determination of propofol in low-volume samples by high-performance liquid chromatography with fluorescence detection

C. A.J. Knibbe; V. S. Koster; V. H.M. Deneer; R. M. Stuurman; P. F.M. Kuks; R. Lange

doi:10.1016/S0378-4347(97)00571-9

Determination of propofol in low-volume samples by high-performance liquid chromatography with fluorescence detection

C. A.J. Knibbe^*, V. S. Koster, V. H.M. Deneer, R. M. Stuurman, P. F.M. Kuks, R. Lange

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In order to determine propofol in rat whole-blood samples of 50 μl, we developed a rapid, simple and reliable method which is characterized by precipitation of blood elements with acetonitrile and submission of the supernatant to HPLC analysis with fluorescence detection. The method described is linear from 0.4 to 40 mg/l and the relative standard deviations in this concentration range are less than 10%. The limit of quantification proved to be 0.4 mg/l. Blood constituents do not interfere with the assay.

Original language	English
Pages (from-to)	305-310
Number of pages	6
Journal	Journal of Chromatography B: Biomedical Applications
Volume	706
Issue number	2
DOIs	https://doi.org/10.1016/S0378-4347(97)00571-9
Publication status	Published - 20 Mar 1998

Keywords

Propofol

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10.1016/S0378-4347(97)00571-9

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title = "Determination of propofol in low-volume samples by high-performance liquid chromatography with fluorescence detection",

abstract = "In order to determine propofol in rat whole-blood samples of 50 μl, we developed a rapid, simple and reliable method which is characterized by precipitation of blood elements with acetonitrile and submission of the supernatant to HPLC analysis with fluorescence detection. The method described is linear from 0.4 to 40 mg/l and the relative standard deviations in this concentration range are less than 10%. The limit of quantification proved to be 0.4 mg/l. Blood constituents do not interfere with the assay.",

keywords = "Propofol",

author = "Knibbe, {C. A.J.} and Koster, {V. S.} and Deneer, {V. H.M.} and Stuurman, {R. M.} and Kuks, {P. F.M.} and R. Lange",

year = "1998",

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doi = "10.1016/S0378-4347(97)00571-9",

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T1 - Determination of propofol in low-volume samples by high-performance liquid chromatography with fluorescence detection

AU - Knibbe, C. A.J.

AU - Koster, V. S.

AU - Deneer, V. H.M.

AU - Stuurman, R. M.

AU - Kuks, P. F.M.

AU - Lange, R.

PY - 1998/3/20

Y1 - 1998/3/20

N2 - In order to determine propofol in rat whole-blood samples of 50 μl, we developed a rapid, simple and reliable method which is characterized by precipitation of blood elements with acetonitrile and submission of the supernatant to HPLC analysis with fluorescence detection. The method described is linear from 0.4 to 40 mg/l and the relative standard deviations in this concentration range are less than 10%. The limit of quantification proved to be 0.4 mg/l. Blood constituents do not interfere with the assay.

AB - In order to determine propofol in rat whole-blood samples of 50 μl, we developed a rapid, simple and reliable method which is characterized by precipitation of blood elements with acetonitrile and submission of the supernatant to HPLC analysis with fluorescence detection. The method described is linear from 0.4 to 40 mg/l and the relative standard deviations in this concentration range are less than 10%. The limit of quantification proved to be 0.4 mg/l. Blood constituents do not interfere with the assay.

KW - Propofol

UR - http://www.scopus.com/inward/record.url?scp=0032549481&partnerID=8YFLogxK

U2 - 10.1016/S0378-4347(97)00571-9

DO - 10.1016/S0378-4347(97)00571-9

M3 - Article

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AN - SCOPUS:0032549481

SN - 1572-6495

VL - 706

SP - 305

EP - 310

JO - Journal of Chromatography B: Biomedical Applications

JF - Journal of Chromatography B: Biomedical Applications

IS - 2

ER -

Determination of propofol in low-volume samples by high-performance liquid chromatography with fluorescence detection

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Keywords

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