DETECTION OF TOXIGENIC CLOSTRIDIUM-DIFFICILE IN FECAL SAMPLES BY COLONY BLOT HYBRIDIZATION

MJHM WOLFHAGEN; AC FLUIT; M JANSZE; CMA RADEMAKER; J VERHOEF

DETECTION OF TOXIGENIC CLOSTRIDIUM-DIFFICILE IN FECAL SAMPLES BY COLONY BLOT HYBRIDIZATION

MJHM WOLFHAGEN^*
, AC FLUIT
, M JANSZE
, CMA RADEMAKER
, J VERHOEF

^*Corresponding author for this work

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

Abstract

A hybridization assay for detection of toxigenic Clostridium difficile in fecal samples was developed and compared with the classical tissue culture cytotoxicity assay. A DNA fragment probe specific for the toxin B gene of Clostridium difficile was synthesized by the polymerase chain reaction and labelled with digoxigenin. Fecal samples were cultured for 24 hours, replica-plated and hybridized with the probe. The hybridization assay had a sensitivity of 100 %, specificity of 96.7 %, positive predictive value of 86.7 % and negative predictive value of 100 % compared with the cytotoxicity assay.

Original language	English
Pages (from-to)	463-466
Number of pages	4
Journal	European Journal of Clinical Microbiology & Infectious Diseases
Volume	12
Issue number	6
Publication status	Published - Jun 1993

Keywords

POLYMERASE CHAIN-REACTION
TOXIN-A
LATEX TEST
IDENTIFICATION
STRAINS
GENE

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@article{77c119ca4b924be888b8912115eec60f,

title = "DETECTION OF TOXIGENIC CLOSTRIDIUM-DIFFICILE IN FECAL SAMPLES BY COLONY BLOT HYBRIDIZATION",

abstract = "A hybridization assay for detection of toxigenic Clostridium difficile in fecal samples was developed and compared with the classical tissue culture cytotoxicity assay. A DNA fragment probe specific for the toxin B gene of Clostridium difficile was synthesized by the polymerase chain reaction and labelled with digoxigenin. Fecal samples were cultured for 24 hours, replica-plated and hybridized with the probe. The hybridization assay had a sensitivity of 100 \%, specificity of 96.7 \%, positive predictive value of 86.7 \% and negative predictive value of 100 \% compared with the cytotoxicity assay.",

keywords = "POLYMERASE CHAIN-REACTION, TOXIN-A, LATEX TEST, IDENTIFICATION, STRAINS, GENE",

author = "MJHM WOLFHAGEN and AC FLUIT and M JANSZE and CMA RADEMAKER and J VERHOEF",

year = "1993",

month = jun,

language = "English",

volume = "12",

pages = "463--466",

journal = "European Journal of Clinical Microbiology \& Infectious Diseases",

issn = "0934-9723",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "6",

}

TY - JOUR

T1 - DETECTION OF TOXIGENIC CLOSTRIDIUM-DIFFICILE IN FECAL SAMPLES BY COLONY BLOT HYBRIDIZATION

AU - WOLFHAGEN, MJHM

AU - FLUIT, AC

AU - JANSZE, M

AU - RADEMAKER, CMA

AU - VERHOEF, J

PY - 1993/6

Y1 - 1993/6

N2 - A hybridization assay for detection of toxigenic Clostridium difficile in fecal samples was developed and compared with the classical tissue culture cytotoxicity assay. A DNA fragment probe specific for the toxin B gene of Clostridium difficile was synthesized by the polymerase chain reaction and labelled with digoxigenin. Fecal samples were cultured for 24 hours, replica-plated and hybridized with the probe. The hybridization assay had a sensitivity of 100 %, specificity of 96.7 %, positive predictive value of 86.7 % and negative predictive value of 100 % compared with the cytotoxicity assay.

AB - A hybridization assay for detection of toxigenic Clostridium difficile in fecal samples was developed and compared with the classical tissue culture cytotoxicity assay. A DNA fragment probe specific for the toxin B gene of Clostridium difficile was synthesized by the polymerase chain reaction and labelled with digoxigenin. Fecal samples were cultured for 24 hours, replica-plated and hybridized with the probe. The hybridization assay had a sensitivity of 100 %, specificity of 96.7 %, positive predictive value of 86.7 % and negative predictive value of 100 % compared with the cytotoxicity assay.

KW - POLYMERASE CHAIN-REACTION

KW - TOXIN-A

KW - LATEX TEST

KW - IDENTIFICATION

KW - STRAINS

KW - GENE

M3 - Comment/Letter to the editor

SN - 0934-9723

VL - 12

SP - 463

EP - 466

JO - European Journal of Clinical Microbiology & Infectious Diseases

JF - European Journal of Clinical Microbiology & Infectious Diseases

IS - 6

ER -

DETECTION OF TOXIGENIC CLOSTRIDIUM-DIFFICILE IN FECAL SAMPLES BY COLONY BLOT HYBRIDIZATION

Abstract

Keywords

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