Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections

Catherine Rabouille

doi:10.1007/978-1-4939-7213-5_11

Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections

Catherine Rabouille

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic

Abstract

The use of tagged RNA probes to directly hybridize frozen sections of chemically fixed tissues, followed by the tag detection with specific antibodies and gold conjugates form the core of the in situ hybridization (ISH)-immunoelectron microscopy (IEM) method that we have developed and successfully used to detect endogenous gurken and bicoid mRNAs in Drosophila oocytes.

Original language	English
Title of host publication	RNA Detection
Subtitle of host publication	methods and protocols
Publisher	Humana Press
Chapter	11
Pages	177-186
Number of pages	10
ISBN (Electronic)	978-1-4939-7213-5
ISBN (Print)	978-1-4939-7212-8
DOIs	https://doi.org/10.1007/978-1-4939-7213-5_11
Publication status	Published - 2018

Publication series

Name	Methods in molecular biology
Publisher	Humana Press
Volume	1649
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Electron microscopy
Frozen sections
ISH-IEM
Immunoelectron microscopy
In situ hybridization
Ultrastructure
mRNA detection

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10.1007/978-1-4939-7213-5_11

Cite this

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abstract = "The use of tagged RNA probes to directly hybridize frozen sections of chemically fixed tissues, followed by the tag detection with specific antibodies and gold conjugates form the core of the in situ hybridization (ISH)-immunoelectron microscopy (IEM) method that we have developed and successfully used to detect endogenous gurken and bicoid mRNAs in Drosophila oocytes.",

keywords = "Electron microscopy, Frozen sections, ISH-IEM, Immunoelectron microscopy, In situ hybridization, Ultrastructure, mRNA detection",

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AB - The use of tagged RNA probes to directly hybridize frozen sections of chemically fixed tissues, followed by the tag detection with specific antibodies and gold conjugates form the core of the in situ hybridization (ISH)-immunoelectron microscopy (IEM) method that we have developed and successfully used to detect endogenous gurken and bicoid mRNAs in Drosophila oocytes.

KW - Electron microscopy

KW - Frozen sections

KW - ISH-IEM

KW - Immunoelectron microscopy

KW - In situ hybridization

KW - Ultrastructure

KW - mRNA detection

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T3 - Methods in molecular biology

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BT - RNA Detection

PB - Humana Press

ER -

Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections

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