Demonstration of migration inhibitory factor (MIF) in a murine system using myelomonocytic leukemia cells (WEHI-3)

M C Kersten; E Pels; R A De Weger; W Den Otter

doi:10.1016/0022-1759(80)90008-3

Demonstration of migration inhibitory factor (MIF) in a murine system using myelomonocytic leukemia cells (WEHI-3)

M C Kersten
, E Pels
, R A De Weger
, W Den Otter

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Cells of the myelomonocytic tumor cell line, WEHI-3, were used as indicator cells in the indirect capillary test for the detection of migration inhibitory factor (MIF). A migration inhibition of about 50% was found and the results were highly reproducible. The indicator cells can be obtained in large quantities, as the myelomonocytic cells grow as an ascitic tumor in the peritoneal cavity of BALB/c mice.

Original language	English
Pages (from-to)	387-90
Number of pages	4
Journal	Journal of Immunological Methods
Volume	33
Issue number	4
DOIs	https://doi.org/10.1016/0022-1759(80)90008-3
Publication status	Published - 1980

Keywords

Animals
Cell Line
Leukemia, Myeloid
Leukocyte Migration-Inhibitory Factors
Lymph Nodes
Lymphokines
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Sheep

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10.1016/0022-1759(80)90008-3

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title = "Demonstration of migration inhibitory factor (MIF) in a murine system using myelomonocytic leukemia cells (WEHI-3)",

abstract = "Cells of the myelomonocytic tumor cell line, WEHI-3, were used as indicator cells in the indirect capillary test for the detection of migration inhibitory factor (MIF). A migration inhibition of about 50\% was found and the results were highly reproducible. The indicator cells can be obtained in large quantities, as the myelomonocytic cells grow as an ascitic tumor in the peritoneal cavity of BALB/c mice.",

keywords = "Animals, Cell Line, Leukemia, Myeloid, Leukocyte Migration-Inhibitory Factors, Lymph Nodes, Lymphokines, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Sheep",

author = "Kersten, \{M C\} and E Pels and \{De Weger\}, \{R A\} and \{Den Otter\}, W",

year = "1980",

doi = "10.1016/0022-1759(80)90008-3",

language = "English",

volume = "33",

pages = "387--90",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "4",

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TY - JOUR

T1 - Demonstration of migration inhibitory factor (MIF) in a murine system using myelomonocytic leukemia cells (WEHI-3)

AU - Kersten, M C

AU - Pels, E

AU - De Weger, R A

AU - Den Otter, W

PY - 1980

Y1 - 1980

N2 - Cells of the myelomonocytic tumor cell line, WEHI-3, were used as indicator cells in the indirect capillary test for the detection of migration inhibitory factor (MIF). A migration inhibition of about 50% was found and the results were highly reproducible. The indicator cells can be obtained in large quantities, as the myelomonocytic cells grow as an ascitic tumor in the peritoneal cavity of BALB/c mice.

AB - Cells of the myelomonocytic tumor cell line, WEHI-3, were used as indicator cells in the indirect capillary test for the detection of migration inhibitory factor (MIF). A migration inhibition of about 50% was found and the results were highly reproducible. The indicator cells can be obtained in large quantities, as the myelomonocytic cells grow as an ascitic tumor in the peritoneal cavity of BALB/c mice.

KW - Animals

KW - Cell Line

KW - Leukemia, Myeloid

KW - Leukocyte Migration-Inhibitory Factors

KW - Lymph Nodes

KW - Lymphokines

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Inbred C57BL

KW - Sheep

U2 - 10.1016/0022-1759(80)90008-3

DO - 10.1016/0022-1759(80)90008-3

M3 - Article

C2 - 6989921

SN - 0022-1759

VL - 33

SP - 387

EP - 390

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 4

ER -

Demonstration of migration inhibitory factor (MIF) in a murine system using myelomonocytic leukemia cells (WEHI-3)

Abstract

Keywords

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