DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells

Martin Harterink; Marta Esteves da Silva; Lena Will; Julia Turan; Adiljan Ibrahim; Alexander E Lang; Eljo Y van Battum; R Jeroen Pasterkamp; Lukas C Kapitein; Dmitri Kudryashov; Ben A Barres; Casper C Hoogenraad; J Bradley Zuchero

doi:10.1038/nmeth.4257

DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells

Martin Harterink^*
, Marta Esteves da Silva
, Lena Will
, Julia Turan
, Adiljan Ibrahim
, Alexander E Lang
, Eljo Y van Battum
, R Jeroen Pasterkamp
, Lukas C Kapitein
, Dmitri Kudryashov
, Ben A Barres
, Casper C Hoogenraad
, J Bradley Zuchero

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.

Original language	English
Pages (from-to)	479-482
Number of pages	4
Journal	Nature Methods
Volume	14
Issue number	5
DOIs	https://doi.org/10.1038/nmeth.4257
Publication status	Published - 2017

Keywords

ADP Ribose Transferases
Actin Cytoskeleton
Actins
Animals
Fibroblasts
Gelsolin
Green Fluorescent Proteins
HeLa Cells
Humans
Peptides
Rats
Recombinant Fusion Proteins
Transfection
Virulence Factors
Journal Article

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10.1038/nmeth.4257

Cite this

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abstract = "The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.",

keywords = "ADP Ribose Transferases, Actin Cytoskeleton, Actins, Animals, Fibroblasts, Gelsolin, Green Fluorescent Proteins, HeLa Cells, Humans, Peptides, Rats, Recombinant Fusion Proteins, Transfection, Virulence Factors, Journal Article",

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year = "2017",

doi = "10.1038/nmeth.4257",

language = "English",

volume = "14",

pages = "479--482",

journal = "Nature Methods",

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T2 - genetically encoded tools for perturbing the actin cytoskeleton in single cells

AU - Harterink, Martin

AU - da Silva, Marta Esteves

AU - Will, Lena

AU - Turan, Julia

AU - Ibrahim, Adiljan

AU - Lang, Alexander E

AU - van Battum, Eljo Y

AU - Pasterkamp, R Jeroen

AU - Kapitein, Lukas C

AU - Kudryashov, Dmitri

AU - Barres, Ben A

AU - Hoogenraad, Casper C

AU - Zuchero, J Bradley

PY - 2017

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AB - The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.

KW - ADP Ribose Transferases

KW - Actin Cytoskeleton

KW - Actins

KW - Animals

KW - Fibroblasts

KW - Gelsolin

KW - Green Fluorescent Proteins

KW - HeLa Cells

KW - Humans

KW - Peptides

KW - Rats

KW - Recombinant Fusion Proteins

KW - Transfection

KW - Virulence Factors

KW - Journal Article

UR - https://www.scopus.com/pages/publications/85017246828

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DO - 10.1038/nmeth.4257

M3 - Article

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VL - 14

SP - 479

EP - 482

JO - Nature Methods

JF - Nature Methods

IS - 5

ER -

DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells

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Keywords

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