Cytokeratin and CDX-2 expression in Barrett's esophagus

J.W.P.M. van Baal; A. Bozikas; R. Pronk; F.J.W. ten Kate; F. Milano; A.M. Rygiel; W.D. Rosmolen; M.P. Peppelenbosch; J.J. Bergman; K.K. Krishnadath

doi:10.1080/00365520701676575

Cytokeratin and CDX-2 expression in Barrett's esophagus

J.W.P.M. van Baal, A. Bozikas, R. Pronk, F.J.W. ten Kate, F. Milano, A.M. Rygiel, W.D. Rosmolen, M.P. Peppelenbosch, J.J. Bergman, K.K. Krishnadath

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Objective. Barrett's esophagus (BE) is a premalignant condition of the distal esophagus. For diagnostic purposes it is important to find biomarkers that can specifically identify BE, for instance to differentiate BE epithelial cells from gastric cardia epithelial cells in brush cytology specimens. The objective of this study was to determine the specificity of CDX-2 and a set of cytokeratins (CKs) as specific markers for BE as compared with normal squamous esophageal and gastric cardia tissue. Material and methods. Immunohistochemistry (IHC) with specific antibodies against CDX-2, and a set of CKs was performed on fresh frozen consecutive tissue sections of normal squamous, gastric cardia and non-dysplastic BE of 80 patients. Results. IHC results showed CK8, CK18 and CK20 expression in both BE and gastric cardia, while CK7 was seen in all BE but also in 26% of gastric cardia biopsies. CK10/13 was only expressed in normal squamous epithelium. CDX-2 nuclear staining was found in 87.5% of the BE biopsies, whereas normal squamous esophagus and cardia biopsies were negative. Conclusions. CDX-2 in combination with a set of CKs can be used as biomarkers to distinguish between BE and normal squamous esophagus. In order to distinguish BE from cardia tissue, a combination of CDX-2 and CK7 is most informative.

Original language	English
Pages (from-to)	132-140
Number of pages	9
Journal	Scandinavian Journal of Gastroenterology
Volume	43
Issue number	2
DOIs	https://doi.org/10.1080/00365520701676575
Publication status	Published - 2008

Keywords

Barrett's esophagus
cytokeratin
CDX-2
HOMEODOMAIN PROTEIN CDX2
HEPATOCYTE NUCLEAR FACTOR-1-ALPHA
INTESTINAL METAPLASIA
GENE-EXPRESSION
GASTROESOPHAGEAL-REFLUX
ECTOPIC EXPRESSION
RISK-FACTORS
ADENOCARCINOMA
MARKER
DIFFERENTIATION

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10.1080/00365520701676575

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@article{a82363d0bd2b4cf284d1b9e0e0ae0064,

title = "Cytokeratin and CDX-2 expression in Barrett's esophagus",

abstract = "Objective. Barrett's esophagus (BE) is a premalignant condition of the distal esophagus. For diagnostic purposes it is important to find biomarkers that can specifically identify BE, for instance to differentiate BE epithelial cells from gastric cardia epithelial cells in brush cytology specimens. The objective of this study was to determine the specificity of CDX-2 and a set of cytokeratins (CKs) as specific markers for BE as compared with normal squamous esophageal and gastric cardia tissue. Material and methods. Immunohistochemistry (IHC) with specific antibodies against CDX-2, and a set of CKs was performed on fresh frozen consecutive tissue sections of normal squamous, gastric cardia and non-dysplastic BE of 80 patients. Results. IHC results showed CK8, CK18 and CK20 expression in both BE and gastric cardia, while CK7 was seen in all BE but also in 26% of gastric cardia biopsies. CK10/13 was only expressed in normal squamous epithelium. CDX-2 nuclear staining was found in 87.5% of the BE biopsies, whereas normal squamous esophagus and cardia biopsies were negative. Conclusions. CDX-2 in combination with a set of CKs can be used as biomarkers to distinguish between BE and normal squamous esophagus. In order to distinguish BE from cardia tissue, a combination of CDX-2 and CK7 is most informative.",

keywords = "Barrett's esophagus, cytokeratin, CDX-2, HOMEODOMAIN PROTEIN CDX2, HEPATOCYTE NUCLEAR FACTOR-1-ALPHA, INTESTINAL METAPLASIA, GENE-EXPRESSION, GASTROESOPHAGEAL-REFLUX, ECTOPIC EXPRESSION, RISK-FACTORS, ADENOCARCINOMA, MARKER, DIFFERENTIATION",

author = "{van Baal}, J.W.P.M. and A. Bozikas and R. Pronk and {ten Kate}, F.J.W. and F. Milano and A.M. Rygiel and W.D. Rosmolen and M.P. Peppelenbosch and J.J. Bergman and K.K. Krishnadath",

year = "2008",

doi = "10.1080/00365520701676575",

language = "English",

volume = "43",

pages = "132--140",

journal = "Scandinavian Journal of Gastroenterology",

issn = "0036-5521",

publisher = "Informa Healthcare",

number = "2",

}

TY - JOUR

T1 - Cytokeratin and CDX-2 expression in Barrett's esophagus

AU - van Baal, J.W.P.M.

AU - Bozikas, A.

AU - Pronk, R.

AU - ten Kate, F.J.W.

AU - Milano, F.

AU - Rygiel, A.M.

AU - Rosmolen, W.D.

AU - Peppelenbosch, M.P.

AU - Bergman, J.J.

AU - Krishnadath, K.K.

PY - 2008

Y1 - 2008

N2 - Objective. Barrett's esophagus (BE) is a premalignant condition of the distal esophagus. For diagnostic purposes it is important to find biomarkers that can specifically identify BE, for instance to differentiate BE epithelial cells from gastric cardia epithelial cells in brush cytology specimens. The objective of this study was to determine the specificity of CDX-2 and a set of cytokeratins (CKs) as specific markers for BE as compared with normal squamous esophageal and gastric cardia tissue. Material and methods. Immunohistochemistry (IHC) with specific antibodies against CDX-2, and a set of CKs was performed on fresh frozen consecutive tissue sections of normal squamous, gastric cardia and non-dysplastic BE of 80 patients. Results. IHC results showed CK8, CK18 and CK20 expression in both BE and gastric cardia, while CK7 was seen in all BE but also in 26% of gastric cardia biopsies. CK10/13 was only expressed in normal squamous epithelium. CDX-2 nuclear staining was found in 87.5% of the BE biopsies, whereas normal squamous esophagus and cardia biopsies were negative. Conclusions. CDX-2 in combination with a set of CKs can be used as biomarkers to distinguish between BE and normal squamous esophagus. In order to distinguish BE from cardia tissue, a combination of CDX-2 and CK7 is most informative.

AB - Objective. Barrett's esophagus (BE) is a premalignant condition of the distal esophagus. For diagnostic purposes it is important to find biomarkers that can specifically identify BE, for instance to differentiate BE epithelial cells from gastric cardia epithelial cells in brush cytology specimens. The objective of this study was to determine the specificity of CDX-2 and a set of cytokeratins (CKs) as specific markers for BE as compared with normal squamous esophageal and gastric cardia tissue. Material and methods. Immunohistochemistry (IHC) with specific antibodies against CDX-2, and a set of CKs was performed on fresh frozen consecutive tissue sections of normal squamous, gastric cardia and non-dysplastic BE of 80 patients. Results. IHC results showed CK8, CK18 and CK20 expression in both BE and gastric cardia, while CK7 was seen in all BE but also in 26% of gastric cardia biopsies. CK10/13 was only expressed in normal squamous epithelium. CDX-2 nuclear staining was found in 87.5% of the BE biopsies, whereas normal squamous esophagus and cardia biopsies were negative. Conclusions. CDX-2 in combination with a set of CKs can be used as biomarkers to distinguish between BE and normal squamous esophagus. In order to distinguish BE from cardia tissue, a combination of CDX-2 and CK7 is most informative.

KW - Barrett's esophagus

KW - cytokeratin

KW - CDX-2

KW - HOMEODOMAIN PROTEIN CDX2

KW - HEPATOCYTE NUCLEAR FACTOR-1-ALPHA

KW - INTESTINAL METAPLASIA

KW - GENE-EXPRESSION

KW - GASTROESOPHAGEAL-REFLUX

KW - ECTOPIC EXPRESSION

KW - RISK-FACTORS

KW - ADENOCARCINOMA

KW - MARKER

KW - DIFFERENTIATION

U2 - 10.1080/00365520701676575

DO - 10.1080/00365520701676575

M3 - Article

C2 - 18224560

SN - 0036-5521

VL - 43

SP - 132

EP - 140

JO - Scandinavian Journal of Gastroenterology

JF - Scandinavian Journal of Gastroenterology

IS - 2

ER -

Cytokeratin and CDX-2 expression in Barrett's esophagus

Abstract

Keywords

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