CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

Delilah Hendriks; Hans Clevers; Benedetta Artegiani

doi:10.1016/j.stem.2020.10.014

CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

Delilah Hendriks
, Hans Clevers
, Benedetta Artegiani

Research output: Contribution to journal › Review article › peer-review

Abstract

CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.

Original language	English
Pages (from-to)	705-731
Number of pages	27
Journal	Cell stem cell
Volume	27
Issue number	5
DOIs	https://doi.org/10.1016/j.stem.2020.10.014
Publication status	Published - 5 Nov 2020

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10.1016/j.stem.2020.10.014

1-s2.0-S1934590920305087-mainFinal published version, 3.24 MBLicence: Taverne

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abstract = "CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.",

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AU - Clevers, Hans

AU - Artegiani, Benedetta

N1 - Funding Information: The authors wish to thank Kadi Lõhmussaar for critical reading of the manuscript. D.H. is supported by a SSMF postdoctoral fellowship ( P19-0074 ). Publisher Copyright: © 2020 Elsevier Inc. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/11/5

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N2 - CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.

AB - CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.

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DO - 10.1016/j.stem.2020.10.014

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JO - Cell stem cell

JF - Cell stem cell

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CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

Abstract

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