TY - JOUR
T1 - CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids
AU - Hendriks, Delilah
AU - Clevers, Hans
AU - Artegiani, Benedetta
N1 - Funding Information:
The authors wish to thank Kadi Lõhmussaar for critical reading of the manuscript. D.H. is supported by a SSMF postdoctoral fellowship ( P19-0074 ).
Publisher Copyright:
© 2020 Elsevier Inc.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11/5
Y1 - 2020/11/5
N2 - CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.
AB - CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.
UR - http://www.scopus.com/inward/record.url?scp=85094946103&partnerID=8YFLogxK
U2 - 10.1016/j.stem.2020.10.014
DO - 10.1016/j.stem.2020.10.014
M3 - Review article
C2 - 33157047
SN - 1934-5909
VL - 27
SP - 705
EP - 731
JO - Cell stem cell
JF - Cell stem cell
IS - 5
ER -