TY - JOUR
T1 - Coxiella burnetii in non-Hodgkin lymphoma tissue samples
T2 - Innocent until proven otherwise?
AU - van Roeden, Sonja E.
AU - Hermans, Mirjam H.A.
AU - Nooijen, Peet T.G.A.
AU - Herbers, Alexandra
AU - Bleeker-Rovers, Chantal P.
AU - Hoepelman, Andy I.M.
AU - Oosterheert, Jan Jelrik
AU - Wever, Peter C.
N1 - Funding Information:
Sonja E. van Roeden, Chantal P. Bleeker-Rovers, Jan Jelrik Oosterheert and Peter C. Wever received a research grant from Foundation Q-support. Sonja E. van Roeden and Jan Jelrik Oosterheert received a research grant from Institut Mérieux. Sonja E. van Roeden, Chantal P. Bleeker-Rovers, Jan Jelrik Oosterheert and Peter C. Wever declare no other competing (financial) interests. Mirjam H.A. Hermans, Peet T.G.A. Nooijen, Alexandra Herbers and Andy I.M. Hoepelman declare no other competing (financial) interests.
Publisher Copyright:
© 2018 Elsevier GmbH
PY - 2019/3
Y1 - 2019/3
N2 - Purpose: Coxiella burnetii has been suggested as a potential cause of B-cell non-Hodgkin lymphoma (B-NHL), as C. burnetii was detected in B-NHL tissues. To further investigate this potential relationship, we hypothesized that among subjects previously exposed to C. burnetii, the bacterium is more frequently detectable in tissues of patients with B-NHL (cases) compared to patients without B-NHL (controls). Methods: We aimed to evaluate this hypothesis by assessing the presence of C. burnetii with polymerase chain reaction (PCR), immunofluorescence staining (IF) and fluorescent in-situ hybridization (FISH). Eligible patients were those previously exposed to C. burnetii. Results: Samples were available for 13 cases and 16 controls. C. burnetii was demonstrated in tissues of 8/29 patients in total (28%), with either PCR, IF or FISH: in 5/13 cases (38%) and 3/16 controls (19%), p = 0.41. Negative and positive control samples were all negative and positive appropriately for all three diagnostic methods. Conclusions: In patients previously exposed to C. burnetii the bacterium was detected in tissue samples from subjects with and without B-NHL, without significant differences in the proportion positive samples. Therefore, we conclude that detection of C. burnetii in tissues of patients previously exposed to C. burnetii is a non-specific finding.
AB - Purpose: Coxiella burnetii has been suggested as a potential cause of B-cell non-Hodgkin lymphoma (B-NHL), as C. burnetii was detected in B-NHL tissues. To further investigate this potential relationship, we hypothesized that among subjects previously exposed to C. burnetii, the bacterium is more frequently detectable in tissues of patients with B-NHL (cases) compared to patients without B-NHL (controls). Methods: We aimed to evaluate this hypothesis by assessing the presence of C. burnetii with polymerase chain reaction (PCR), immunofluorescence staining (IF) and fluorescent in-situ hybridization (FISH). Eligible patients were those previously exposed to C. burnetii. Results: Samples were available for 13 cases and 16 controls. C. burnetii was demonstrated in tissues of 8/29 patients in total (28%), with either PCR, IF or FISH: in 5/13 cases (38%) and 3/16 controls (19%), p = 0.41. Negative and positive control samples were all negative and positive appropriately for all three diagnostic methods. Conclusions: In patients previously exposed to C. burnetii the bacterium was detected in tissue samples from subjects with and without B-NHL, without significant differences in the proportion positive samples. Therefore, we conclude that detection of C. burnetii in tissues of patients previously exposed to C. burnetii is a non-specific finding.
KW - B-cell non-Hodgkin lymphoma
KW - Coxiella burnetii
KW - Non-Hodgkin lymphoma
KW - Q fever
UR - https://www.scopus.com/pages/publications/85059665913
U2 - 10.1016/j.imbio.2018.11.012
DO - 10.1016/j.imbio.2018.11.012
M3 - Article
C2 - 30638649
AN - SCOPUS:85059665913
SN - 0171-2985
VL - 224
SP - 254
EP - 261
JO - Immunobiology
JF - Immunobiology
IS - 2
ER -