Correlation between detection methods of Chlamydia pneumoniae in atherosclerotic and non-atherosclerotic tissues

B. Maraha; H. Berg; G. J. Scheffer; A. Van der Zee; A. Bergmans; J. Miseré; J. Kluytmans; M. Peeters

doi:10.1016/S0732-8893(01)00212-7

Correlation between detection methods of Chlamydia pneumoniae in atherosclerotic and non-atherosclerotic tissues

B. Maraha^*
, H. Berg
, G. J. Scheffer
, A. Van der Zee
, A. Bergmans
, J. Miseré
, J. Kluytmans
, M. Peeters

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

13 Citations (Scopus)

Abstract

Polymerase chain reaction (PCR) and immunohistochemistry (IHC) have been used to detect Chlamydia (C.) pneumoniae in vascular tissues. Discrepancies between the results of these two methods have frequently been reported. However, the correlation between PCR and IHC has not been analyzed yet. This study assesses the correlation between the detection of C. pneumoniae by PCR and IHC in 45 atherosclerotic and 50 non-atherosclerotic tissue specimens. Also, the presence of Mycoplasma (M.) pneumoniae in these 95 specimens was investigated. Correlation was found between the detection of C. pneumoniae by PCR and IHC in the atherosclerotic tissues. Both tests were positive in 10 specimens and negative in 17 specimens (p = 0.003). There was no significant correlation between PCR and IHC in non-atherosclerotic specimens (p = ns). M. pneumoniae was detected, by PCR, in one atherosclerotic specimen. The results show correlation between PCR and IHC in the detection of C. pneumoniae in atherosclerotic tissues, emphasize the association between C. pneumoniae and atherosclerosis, and support the specificity of the association between C. pneumoniae and atherosclerosis.

Original language	English
Pages (from-to)	139-143
Number of pages	5
Journal	Diagnostic Microbiology and Infectious Disease
Volume	39
Issue number	3
DOIs	https://doi.org/10.1016/S0732-8893(01)00212-7
Publication status	Published - 2001
Externally published	Yes

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10.1016/S0732-8893(01)00212-7

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title = "Correlation between detection methods of Chlamydia pneumoniae in atherosclerotic and non-atherosclerotic tissues",

abstract = "Polymerase chain reaction (PCR) and immunohistochemistry (IHC) have been used to detect Chlamydia (C.) pneumoniae in vascular tissues. Discrepancies between the results of these two methods have frequently been reported. However, the correlation between PCR and IHC has not been analyzed yet. This study assesses the correlation between the detection of C. pneumoniae by PCR and IHC in 45 atherosclerotic and 50 non-atherosclerotic tissue specimens. Also, the presence of Mycoplasma (M.) pneumoniae in these 95 specimens was investigated. Correlation was found between the detection of C. pneumoniae by PCR and IHC in the atherosclerotic tissues. Both tests were positive in 10 specimens and negative in 17 specimens (p = 0.003). There was no significant correlation between PCR and IHC in non-atherosclerotic specimens (p = ns). M. pneumoniae was detected, by PCR, in one atherosclerotic specimen. The results show correlation between PCR and IHC in the detection of C. pneumoniae in atherosclerotic tissues, emphasize the association between C. pneumoniae and atherosclerosis, and support the specificity of the association between C. pneumoniae and atherosclerosis.",

author = "B. Maraha and H. Berg and Scheffer, \{G. J.\} and \{Van der Zee\}, A. and A. Bergmans and J. Miser{\'e} and J. Kluytmans and M. Peeters",

year = "2001",

doi = "10.1016/S0732-8893(01)00212-7",

language = "English",

volume = "39",

pages = "139--143",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier",

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TY - JOUR

T1 - Correlation between detection methods of Chlamydia pneumoniae in atherosclerotic and non-atherosclerotic tissues

AU - Maraha, B.

AU - Berg, H.

AU - Scheffer, G. J.

AU - Van der Zee, A.

AU - Bergmans, A.

AU - Miseré, J.

AU - Kluytmans, J.

AU - Peeters, M.

PY - 2001

Y1 - 2001

N2 - Polymerase chain reaction (PCR) and immunohistochemistry (IHC) have been used to detect Chlamydia (C.) pneumoniae in vascular tissues. Discrepancies between the results of these two methods have frequently been reported. However, the correlation between PCR and IHC has not been analyzed yet. This study assesses the correlation between the detection of C. pneumoniae by PCR and IHC in 45 atherosclerotic and 50 non-atherosclerotic tissue specimens. Also, the presence of Mycoplasma (M.) pneumoniae in these 95 specimens was investigated. Correlation was found between the detection of C. pneumoniae by PCR and IHC in the atherosclerotic tissues. Both tests were positive in 10 specimens and negative in 17 specimens (p = 0.003). There was no significant correlation between PCR and IHC in non-atherosclerotic specimens (p = ns). M. pneumoniae was detected, by PCR, in one atherosclerotic specimen. The results show correlation between PCR and IHC in the detection of C. pneumoniae in atherosclerotic tissues, emphasize the association between C. pneumoniae and atherosclerosis, and support the specificity of the association between C. pneumoniae and atherosclerosis.

AB - Polymerase chain reaction (PCR) and immunohistochemistry (IHC) have been used to detect Chlamydia (C.) pneumoniae in vascular tissues. Discrepancies between the results of these two methods have frequently been reported. However, the correlation between PCR and IHC has not been analyzed yet. This study assesses the correlation between the detection of C. pneumoniae by PCR and IHC in 45 atherosclerotic and 50 non-atherosclerotic tissue specimens. Also, the presence of Mycoplasma (M.) pneumoniae in these 95 specimens was investigated. Correlation was found between the detection of C. pneumoniae by PCR and IHC in the atherosclerotic tissues. Both tests were positive in 10 specimens and negative in 17 specimens (p = 0.003). There was no significant correlation between PCR and IHC in non-atherosclerotic specimens (p = ns). M. pneumoniae was detected, by PCR, in one atherosclerotic specimen. The results show correlation between PCR and IHC in the detection of C. pneumoniae in atherosclerotic tissues, emphasize the association between C. pneumoniae and atherosclerosis, and support the specificity of the association between C. pneumoniae and atherosclerosis.

UR - https://www.scopus.com/pages/publications/0035047513

U2 - 10.1016/S0732-8893(01)00212-7

DO - 10.1016/S0732-8893(01)00212-7

M3 - Article

C2 - 11337179

AN - SCOPUS:0035047513

SN - 0732-8893

VL - 39

SP - 139

EP - 143

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 3

ER -

Correlation between detection methods of Chlamydia pneumoniae in atherosclerotic and non-atherosclerotic tissues

Abstract

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