Coronaviruses in polarized epithelial cells

J W Rossen; C P Bekker; W F Voorhout; M C Horzinek; A Van der Ende; G J Strous; P J Rottier

doi:10.1007/978-1-4615-1899-0_22

Coronaviruses in polarized epithelial cells

J W Rossen
, C P Bekker
, W F Voorhout
, M C Horzinek
, A Van der Ende
, G J Strous
, P J Rottier

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Coronaviruses have a marked tropism for epithelial cells. In this paper the interactions of the porcine transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV-A59) with epithelial cells are compared. Porcine (LLC-PK1) and murine (mTAL) epithelial cells were grown on permeable supports. By inoculation from the apical or basolateral side both TGEV and MHV-A59 were found to enter the polarized cells only through the apical membrane. The release of newly synthesized TGEV from LLC-PK1 cells occurred preferentially from the apical plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the apical culture fluid. In contrast, MHV was released preferentially from the basolateral membrane of mTAL cells. The apical release of TGEV and the basolateral release of MHV may explain the in vivo establishment of a local and systemic infection, respectively.

Original language	English
Pages (from-to)	135-8
Number of pages	4
Journal	Advances in Experimental Medicine and Biology
Volume	380
DOIs	https://doi.org/10.1007/978-1-4615-1899-0_22
Publication status	Published - 1995
Externally published	Yes

Keywords

Animals
Autoradiography
Capsid/biosynthesis
Cell Line
Coronavirus/pathogenicity
Electrophoresis, Polyacrylamide Gel
Epithelium/virology
Membrane Glycoproteins/biosynthesis
Methionine/metabolism
Mice
Murine hepatitis virus/pathogenicity
RNA Viruses/pathogenicity
Spike Glycoprotein, Coronavirus
Sulfur Radioisotopes
Swine
Viral Core Proteins/biosynthesis
Viral Envelope Proteins/biosynthesis
Viral Proteins/biosynthesis
Virus Replication

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10.1007/978-1-4615-1899-0_22

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title = "Coronaviruses in polarized epithelial cells",

abstract = "Coronaviruses have a marked tropism for epithelial cells. In this paper the interactions of the porcine transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV-A59) with epithelial cells are compared. Porcine (LLC-PK1) and murine (mTAL) epithelial cells were grown on permeable supports. By inoculation from the apical or basolateral side both TGEV and MHV-A59 were found to enter the polarized cells only through the apical membrane. The release of newly synthesized TGEV from LLC-PK1 cells occurred preferentially from the apical plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the apical culture fluid. In contrast, MHV was released preferentially from the basolateral membrane of mTAL cells. The apical release of TGEV and the basolateral release of MHV may explain the in vivo establishment of a local and systemic infection, respectively.",

keywords = "Animals, Autoradiography, Capsid/biosynthesis, Cell Line, Coronavirus/pathogenicity, Electrophoresis, Polyacrylamide Gel, Epithelium/virology, Membrane Glycoproteins/biosynthesis, Methionine/metabolism, Mice, Murine hepatitis virus/pathogenicity, RNA Viruses/pathogenicity, Spike Glycoprotein, Coronavirus, Sulfur Radioisotopes, Swine, Viral Core Proteins/biosynthesis, Viral Envelope Proteins/biosynthesis, Viral Proteins/biosynthesis, Virus Replication",

author = "Rossen, \{J W\} and Bekker, \{C P\} and Voorhout, \{W F\} and Horzinek, \{M C\} and \{Van der Ende\}, A and Strous, \{G J\} and Rottier, \{P J\}",

year = "1995",

doi = "10.1007/978-1-4615-1899-0\_22",

language = "English",

volume = "380",

pages = "135--8",

journal = "Advances in Experimental Medicine and Biology",

issn = "0065-2598",

publisher = "Springer",

}

TY - JOUR

T1 - Coronaviruses in polarized epithelial cells

AU - Rossen, J W

AU - Bekker, C P

AU - Voorhout, W F

AU - Horzinek, M C

AU - Van der Ende, A

AU - Strous, G J

AU - Rottier, P J

PY - 1995

Y1 - 1995

N2 - Coronaviruses have a marked tropism for epithelial cells. In this paper the interactions of the porcine transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV-A59) with epithelial cells are compared. Porcine (LLC-PK1) and murine (mTAL) epithelial cells were grown on permeable supports. By inoculation from the apical or basolateral side both TGEV and MHV-A59 were found to enter the polarized cells only through the apical membrane. The release of newly synthesized TGEV from LLC-PK1 cells occurred preferentially from the apical plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the apical culture fluid. In contrast, MHV was released preferentially from the basolateral membrane of mTAL cells. The apical release of TGEV and the basolateral release of MHV may explain the in vivo establishment of a local and systemic infection, respectively.

AB - Coronaviruses have a marked tropism for epithelial cells. In this paper the interactions of the porcine transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV-A59) with epithelial cells are compared. Porcine (LLC-PK1) and murine (mTAL) epithelial cells were grown on permeable supports. By inoculation from the apical or basolateral side both TGEV and MHV-A59 were found to enter the polarized cells only through the apical membrane. The release of newly synthesized TGEV from LLC-PK1 cells occurred preferentially from the apical plasma membrane domain, as evidenced by the accumulation of viral proteins and infectivity in the apical culture fluid. In contrast, MHV was released preferentially from the basolateral membrane of mTAL cells. The apical release of TGEV and the basolateral release of MHV may explain the in vivo establishment of a local and systemic infection, respectively.

KW - Animals

KW - Autoradiography

KW - Capsid/biosynthesis

KW - Cell Line

KW - Coronavirus/pathogenicity

KW - Electrophoresis, Polyacrylamide Gel

KW - Epithelium/virology

KW - Membrane Glycoproteins/biosynthesis

KW - Methionine/metabolism

KW - Mice

KW - Murine hepatitis virus/pathogenicity

KW - RNA Viruses/pathogenicity

KW - Spike Glycoprotein, Coronavirus

KW - Sulfur Radioisotopes

KW - Swine

KW - Viral Core Proteins/biosynthesis

KW - Viral Envelope Proteins/biosynthesis

KW - Viral Proteins/biosynthesis

KW - Virus Replication

U2 - 10.1007/978-1-4615-1899-0_22

DO - 10.1007/978-1-4615-1899-0_22

M3 - Article

C2 - 8830469

SN - 0065-2598

VL - 380

SP - 135

EP - 138

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

ER -

Coronaviruses in polarized epithelial cells

Abstract

Keywords

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