Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets

Janneke Hilderink; Siebe Spijker; Francoise Carlotti; Lydia Lange; Marten Engelse; Clemens van Blitterswijk; Eelco de Koning; Marcel Karperien; Aart van Apeldoorn

doi:10.1111/jcmm.12555

Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets

Janneke Hilderink, Siebe Spijker, Francoise Carlotti, Lydia Lange, Marten Engelse, Clemens van Blitterswijk, Eelco de Koning, Marcel Karperien, Aart van Apeldoorn^*

^*Corresponding author for this work

Regenerative Medicine and Stem Cells

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Clinical islet transplantation is a promising treatment for patients with type 1 diabetes. However, pancreatic islets vary in size and shape affecting their survival and function after transplantation because of mass transport limitations. To reduce diffusion restrictions and improve islet cell survival, the generation of islets with optimal dimensions by dispersion followed by reassembly of islet cells, can help limit the length of diffusion pathways. This study describes a microwell platform that supports the controlled and reproducible production of three-dimensional pancreatic cell clusters of human donor islets. We observed that primary human islet cell aggregates with a diameter of 100-150m consisting of about 1000 cells best resembled intact pancreatic islets as they showed low apoptotic cell death (

Original language	English
Pages (from-to)	1836-1846
Number of pages	11
Journal	Journal of Cellular and Molecular Medicine
Volume	19
Issue number	8
DOIs	https://doi.org/10.1111/jcmm.12555
Publication status	Published - Aug 2015

Keywords

pseudoislets bioengineering
beta cells
islets
type 1 diabetes
HUMAN BETA-CELLS
MOLECULE N-CAM
INSULIN-SECRETION
MIN6 PSEUDOISLETS
TRANSPLANTATION
CULTURE
RELEASE
SEGREGATION
EXPRESSION
CLUSTERS

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Hilderink, J., Spijker, S., Carlotti, F., Lange, L., Engelse, M., van Blitterswijk, C., de Koning, E., Karperien, M., & van Apeldoorn, A. (2015). Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets. Journal of Cellular and Molecular Medicine, 19(8), 1836-1846. https://doi.org/10.1111/jcmm.12555

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title = "Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets",

abstract = "Clinical islet transplantation is a promising treatment for patients with type 1 diabetes. However, pancreatic islets vary in size and shape affecting their survival and function after transplantation because of mass transport limitations. To reduce diffusion restrictions and improve islet cell survival, the generation of islets with optimal dimensions by dispersion followed by reassembly of islet cells, can help limit the length of diffusion pathways. This study describes a microwell platform that supports the controlled and reproducible production of three-dimensional pancreatic cell clusters of human donor islets. We observed that primary human islet cell aggregates with a diameter of 100-150m consisting of about 1000 cells best resembled intact pancreatic islets as they showed low apoptotic cell death (",

keywords = "pseudoislets bioengineering, beta cells, islets, type 1 diabetes, HUMAN BETA-CELLS, MOLECULE N-CAM, INSULIN-SECRETION, MIN6 PSEUDOISLETS, TRANSPLANTATION, CULTURE, RELEASE, SEGREGATION, EXPRESSION, CLUSTERS",

author = "Janneke Hilderink and Siebe Spijker and Francoise Carlotti and Lydia Lange and Marten Engelse and {van Blitterswijk}, Clemens and {de Koning}, Eelco and Marcel Karperien and {van Apeldoorn}, Aart",

year = "2015",

month = aug,

doi = "10.1111/jcmm.12555",

language = "English",

volume = "19",

pages = "1836--1846",

journal = "Journal of Cellular and Molecular Medicine",

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Hilderink, J, Spijker, S, Carlotti, F, Lange, L, Engelse, M, van Blitterswijk, C, de Koning, E, Karperien, M & van Apeldoorn, A 2015, 'Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets', Journal of Cellular and Molecular Medicine, vol. 19, no. 8, pp. 1836-1846. https://doi.org/10.1111/jcmm.12555

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T1 - Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets

AU - Hilderink, Janneke

AU - Spijker, Siebe

AU - Carlotti, Francoise

AU - Lange, Lydia

AU - Engelse, Marten

AU - van Blitterswijk, Clemens

AU - de Koning, Eelco

AU - Karperien, Marcel

AU - van Apeldoorn, Aart

PY - 2015/8

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N2 - Clinical islet transplantation is a promising treatment for patients with type 1 diabetes. However, pancreatic islets vary in size and shape affecting their survival and function after transplantation because of mass transport limitations. To reduce diffusion restrictions and improve islet cell survival, the generation of islets with optimal dimensions by dispersion followed by reassembly of islet cells, can help limit the length of diffusion pathways. This study describes a microwell platform that supports the controlled and reproducible production of three-dimensional pancreatic cell clusters of human donor islets. We observed that primary human islet cell aggregates with a diameter of 100-150m consisting of about 1000 cells best resembled intact pancreatic islets as they showed low apoptotic cell death (

AB - Clinical islet transplantation is a promising treatment for patients with type 1 diabetes. However, pancreatic islets vary in size and shape affecting their survival and function after transplantation because of mass transport limitations. To reduce diffusion restrictions and improve islet cell survival, the generation of islets with optimal dimensions by dispersion followed by reassembly of islet cells, can help limit the length of diffusion pathways. This study describes a microwell platform that supports the controlled and reproducible production of three-dimensional pancreatic cell clusters of human donor islets. We observed that primary human islet cell aggregates with a diameter of 100-150m consisting of about 1000 cells best resembled intact pancreatic islets as they showed low apoptotic cell death (

KW - pseudoislets bioengineering

KW - beta cells

KW - islets

KW - type 1 diabetes

KW - HUMAN BETA-CELLS

KW - MOLECULE N-CAM

KW - INSULIN-SECRETION

KW - MIN6 PSEUDOISLETS

KW - TRANSPLANTATION

KW - CULTURE

KW - RELEASE

KW - SEGREGATION

KW - EXPRESSION

KW - CLUSTERS

U2 - 10.1111/jcmm.12555

DO - 10.1111/jcmm.12555

M3 - Article

C2 - 25782016

SN - 1582-4934

VL - 19

SP - 1836

EP - 1846

JO - Journal of Cellular and Molecular Medicine

JF - Journal of Cellular and Molecular Medicine

IS - 8

ER -

Controlled aggregation of primary human pancreatic islet cells leads to glucose-responsive pseudoislets comparable to native islets

Abstract

Keywords

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