TY - JOUR
T1 - Contractility of permeabilized rat vastus intermedius muscle fibres following high-fat, high-sucrose diet consumption
AU - Smith, Ian C.
AU - Ostertag, Curtis
AU - O’reilly, Jennifer J.
AU - Rios, Jaqueline L.
AU - Klancic, Teja
AU - Macdonald, Graham Z.
AU - Collins, Kelsey H.
AU - Reimer, Raylene A.
AU - Herzog, Walter
N1 - Funding Information:
Research was funded by research grants from Canadian Institutes of Health Research (PJT-159626, R.A.R.; FDN-143341, W.H.), the Natural Sciences and Engineering Research Council of Canada (RGPIN/36674-2013, W.H.), Canada Research Chair Programme (950-230603, W.H.), and the Killam foundation (W.H.). Funding support also came from the Vanier Canada Graduate Scholarship program (T.K.), Alberta Innovates (T.K., I.C.S., C.O., K.H.C., J.L.R., G.Z.M.), the University of Calgary Eye’s High program (T.K., G.Z.M.), the Canadian Institutes of Health Research (FRN-152453, I.C.S.; Banting and Best Canada Graduate Scholarship, K.H.C.), the Natural Sciences and Engineering Research Council of Canada CREATE program (J.J.O.), the Alberta Innovates Health Solutions Osteoarthritis Team Grant (K.H.C.), the Killam Foundation (K.H.C.), and the Ministry of Education, Brazil (CAPES Foundation Grant 13157-13-2, J.L.R.). Author contributions: Conceptualization: all authors. Data curation: I.C.S., C.O., J.J.O., J.L.R., T.K., G.Z.M., K.H.C. Formal Analysis: I.C.S., C.O., J.J.O., J.L.R., T.K., G.Z.M., K.H.C. Funding acquisition: RAR WH. Investigation: I.C.S., C.O., J.J.O., J.L.R., T.K., G.Z.M., K.H.C. Methodology: I.C.S., J.L.R., T.K., G.Z.M., K.H.C. Project administration: I.C.S., J.L.R., T.K., G.Z.M., K.H.C., R.A.R., W.H. Resources: J.L.R., T.K., G.Z.M., K.H.C., R.A.R., W.H. Software: I.C.S. Supervision: I.C.S., R.A.R., W.H. Validation: I.C.S., J.L.R., T.K., G.Z.M., K.H.C. Visualization: I.C.S. Writing, original draft: I.C.S. Writing, review and editing: all authors.
Publisher Copyright:
© 2021 The Author(s).
PY - 2021/6
Y1 - 2021/6
N2 - Obesity is a worldwide health concern associated with impaired physical function. It is not clear if contractile protein dysfunction contributes to the impairment of muscle function observed with obesity. The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague–Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort). HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts. Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHCIfibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined. However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort. We conclude that diet-induced obesity can impair force output of permeabilized muscle fibres of adult rats. Novelty: • We assessed contractile function of permeabilized skeletal muscle fibres in a rat model of diet-induced obesity. • The high-fat, high-sucrose diet was associated with impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats. • Other measures of contractile function were not significantly affected by diet.
AB - Obesity is a worldwide health concern associated with impaired physical function. It is not clear if contractile protein dysfunction contributes to the impairment of muscle function observed with obesity. The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague–Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort). HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts. Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHCIfibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined. However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort. We conclude that diet-induced obesity can impair force output of permeabilized muscle fibres of adult rats. Novelty: • We assessed contractile function of permeabilized skeletal muscle fibres in a rat model of diet-induced obesity. • The high-fat, high-sucrose diet was associated with impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats. • Other measures of contractile function were not significantly affected by diet.
KW - Fibre type
KW - Myosin
KW - Obesity
KW - Pre-clinical models
KW - Skeletal muscle
UR - http://www.scopus.com/inward/record.url?scp=85118629744&partnerID=8YFLogxK
U2 - 10.1139/apnm-2021-0238
DO - 10.1139/apnm-2021-0238
M3 - Article
C2 - 34139131
SN - 1715-5312
VL - 46
SP - 1389
EP - 1399
JO - Applied Physiology, Nutrition and Metabolism
JF - Applied Physiology, Nutrition and Metabolism
IS - 11
ER -