Contactomics: Exploring New Dimensions of 3D Genome Architecture

B.A.M. Bouwman

    Research output: ThesisDoctoral thesis 1 (Research UU / Graduation UU)

    Abstract

    3D genome folding is increasingly recognized as an instrumental regulator of gene expression. This thesis contains two literature reviews in which the current knowledge regarding genome folding and function is described; chapter one focusses on the implications for gene regulation at each hierarchical level of genome folding and addresses the factors that are believed to fold the genome at each level. In chapter two, the unique 3D folding of the pluripotent genome is discussed. The three experimental chapters of this thesis describe the results of three studies, aimed to gain a better understanding of the relationship between genome form and function. Chapter three describes specific features of the 3D genome of pluripotent stem cells, and how these are lost and gained during differentiation and reprogramming. Chapter four focusses on the development of a novel method to study genome conformation: multi-contact chromosome conformation capture sequencing, which allows studying of multi-way chromatin conformation and chromatin hubs. The final chapter describes a collaborative study in which the chromatin dynamics of enhancer-promoter contacts are followed during in vitro cardiomyocyte differentiation. Together, the chapters of this thesis illustrate the importance of studying gene expression in the context of the 3D nucleus.
    Original languageEnglish
    Awarding Institution
    • University Medical Center (UMC) Utrecht
    Supervisors/Advisors
    • de Laat, Wouter, Primary supervisor
    Award date23 Jun 2016
    Publisher
    Print ISBNs978-90-393-6571-7
    Publication statusPublished - 23 Jun 2016

    Keywords

    • 3D genome
    • transcription regulation
    • pluripotency
    • stem cell

    Fingerprint

    Dive into the research topics of 'Contactomics: Exploring New Dimensions of 3D Genome Architecture'. Together they form a unique fingerprint.

    Cite this