Comparison of the EUCAST and CLSI Broth Microdilution Methods for Testing Isavuconazole, Posaconazole, and Amphotericin B against Molecularly Identified Mucorales Species

Anuradha Chowdhary; Pradeep Kumar Singh; Shallu Kathuria; Ferry Hagen; Jacques F Meis

doi:10.1128/AAC.02107-15

Comparison of the EUCAST and CLSI Broth Microdilution Methods for Testing Isavuconazole, Posaconazole, and Amphotericin B against Molecularly Identified Mucorales Species

Anuradha Chowdhary^*
, Pradeep Kumar Singh
, Shallu Kathuria
, Ferry Hagen
, Jacques F Meis

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We compared EUCAST and CLSI antifungal susceptibility testing (AFST) methods for triazoles and amphotericin B against 124 clinical Mucorales isolates. The EUCAST method yielded MIC values 1- to 3-fold dilutions higher than those of the CLSI method for amphotericin B. The essential agreements between the two methods for triazoles were high, i.e., 99.1% (voriconazole), 98.3% (isavuconazole), and 87% (posaconazole), whereas it was significantly lower for amphotericin B (66.1%). Strategies for harmonization of the two methods for Mucorales AFST are warranted.

Original language	English
Pages (from-to)	7882-7
Number of pages	6
Journal	Antimicrobial Agents and Chemotherapy
Volume	59
Issue number	12
DOIs	https://doi.org/10.1128/AAC.02107-15
Publication status	Published - Dec 2015
Externally published	Yes

Keywords

Amphotericin B/pharmacology
Antifungal Agents/pharmacology
Humans
Microbial Sensitivity Tests/methods
Mucorales/classification
Mucormycosis/drug therapy
Nitriles/pharmacology
Pyridines/pharmacology
Species Specificity
Triazoles/pharmacology
Voriconazole/pharmacology

Access to Document

10.1128/AAC.02107-15

Cite this

@article{8d6bcb5310be4f6184691adc66b7b6bc,

title = "Comparison of the EUCAST and CLSI Broth Microdilution Methods for Testing Isavuconazole, Posaconazole, and Amphotericin B against Molecularly Identified Mucorales Species",

abstract = "We compared EUCAST and CLSI antifungal susceptibility testing (AFST) methods for triazoles and amphotericin B against 124 clinical Mucorales isolates. The EUCAST method yielded MIC values 1- to 3-fold dilutions higher than those of the CLSI method for amphotericin B. The essential agreements between the two methods for triazoles were high, i.e., 99.1\% (voriconazole), 98.3\% (isavuconazole), and 87\% (posaconazole), whereas it was significantly lower for amphotericin B (66.1\%). Strategies for harmonization of the two methods for Mucorales AFST are warranted.",

keywords = "Amphotericin B/pharmacology, Antifungal Agents/pharmacology, Humans, Microbial Sensitivity Tests/methods, Mucorales/classification, Mucormycosis/drug therapy, Nitriles/pharmacology, Pyridines/pharmacology, Species Specificity, Triazoles/pharmacology, Voriconazole/pharmacology",

author = "Anuradha Chowdhary and Singh, \{Pradeep Kumar\} and Shallu Kathuria and Ferry Hagen and Meis, \{Jacques F\}",

year = "2015",

month = dec,

doi = "10.1128/AAC.02107-15",

language = "English",

volume = "59",

pages = "7882--7",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "12",

}

Comparison of the EUCAST and CLSI Broth Microdilution Methods for Testing Isavuconazole, Posaconazole, and Amphotericin B against Molecularly Identified Mucorales Species. / Chowdhary, Anuradha; Singh, Pradeep Kumar; Kathuria, Shallu et al.
In: Antimicrobial Agents and Chemotherapy, Vol. 59, No. 12, 12.2015, p. 7882-7.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Comparison of the EUCAST and CLSI Broth Microdilution Methods for Testing Isavuconazole, Posaconazole, and Amphotericin B against Molecularly Identified Mucorales Species

AU - Chowdhary, Anuradha

AU - Singh, Pradeep Kumar

AU - Kathuria, Shallu

AU - Hagen, Ferry

AU - Meis, Jacques F

PY - 2015/12

Y1 - 2015/12

N2 - We compared EUCAST and CLSI antifungal susceptibility testing (AFST) methods for triazoles and amphotericin B against 124 clinical Mucorales isolates. The EUCAST method yielded MIC values 1- to 3-fold dilutions higher than those of the CLSI method for amphotericin B. The essential agreements between the two methods for triazoles were high, i.e., 99.1% (voriconazole), 98.3% (isavuconazole), and 87% (posaconazole), whereas it was significantly lower for amphotericin B (66.1%). Strategies for harmonization of the two methods for Mucorales AFST are warranted.

AB - We compared EUCAST and CLSI antifungal susceptibility testing (AFST) methods for triazoles and amphotericin B against 124 clinical Mucorales isolates. The EUCAST method yielded MIC values 1- to 3-fold dilutions higher than those of the CLSI method for amphotericin B. The essential agreements between the two methods for triazoles were high, i.e., 99.1% (voriconazole), 98.3% (isavuconazole), and 87% (posaconazole), whereas it was significantly lower for amphotericin B (66.1%). Strategies for harmonization of the two methods for Mucorales AFST are warranted.

KW - Amphotericin B/pharmacology

KW - Antifungal Agents/pharmacology

KW - Humans

KW - Microbial Sensitivity Tests/methods

KW - Mucorales/classification

KW - Mucormycosis/drug therapy

KW - Nitriles/pharmacology

KW - Pyridines/pharmacology

KW - Species Specificity

KW - Triazoles/pharmacology

KW - Voriconazole/pharmacology

U2 - 10.1128/AAC.02107-15

DO - 10.1128/AAC.02107-15

M3 - Article

C2 - 26438489

SN - 0066-4804

VL - 59

SP - 7882

EP - 7887

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 12

ER -

Comparison of the EUCAST and CLSI Broth Microdilution Methods for Testing Isavuconazole, Posaconazole, and Amphotericin B against Molecularly Identified Mucorales Species

Abstract

Keywords

Access to Document

Fingerprint

Cite this