TY - JOUR
T1 - Comparison of five tests for identification of Staphylococcus aureus from clinical samples
AU - Luijendijk, A. D.
AU - Van Belkum, Alex
AU - Verbrugh, Henri
AU - Kluytmans, Jan
PY - 1996/9
Y1 - 1996/9
N2 - Five different laboratory tests for the identification of Staphylococcus aureus were compared. Analyses of 271 presumptive S. aureus strains, supplemented with 59 well-defined methicillin-resistant S. aureus (MRSA) isolates, were performed. Only the Staphaurex Plus (Murex Diagnostics, Dartford, United Kingdom) and the Pastorex Staphplus (Sanofi, Marnes-La- Coquette, France) tests displayed 100% sensitivity. The observed difference with the free-coagulase test (Bacto coagulase plasma; Difco, Detroit, Mich.), a bound-coagulase (clumping factor) test, and the former Staphaurex test (Murex Diagnostics) was caused mainly by the inability of these three tests to identify some MRSA strains correctly. Among Polish MRSA isolates included in the analysis, a group of free-coagulase-negative S. aureus strains was detected. Genetic typing by random amplification of polymorphic DNA revealed that the strains showing aberrant behavior when the different test results were compared belonged to a limited number of S. aureus clones.
AB - Five different laboratory tests for the identification of Staphylococcus aureus were compared. Analyses of 271 presumptive S. aureus strains, supplemented with 59 well-defined methicillin-resistant S. aureus (MRSA) isolates, were performed. Only the Staphaurex Plus (Murex Diagnostics, Dartford, United Kingdom) and the Pastorex Staphplus (Sanofi, Marnes-La- Coquette, France) tests displayed 100% sensitivity. The observed difference with the free-coagulase test (Bacto coagulase plasma; Difco, Detroit, Mich.), a bound-coagulase (clumping factor) test, and the former Staphaurex test (Murex Diagnostics) was caused mainly by the inability of these three tests to identify some MRSA strains correctly. Among Polish MRSA isolates included in the analysis, a group of free-coagulase-negative S. aureus strains was detected. Genetic typing by random amplification of polymorphic DNA revealed that the strains showing aberrant behavior when the different test results were compared belonged to a limited number of S. aureus clones.
UR - http://www.scopus.com/inward/record.url?scp=0029777354&partnerID=8YFLogxK
U2 - 10.1128/jcm.34.9.2267-2269.1996
DO - 10.1128/jcm.34.9.2267-2269.1996
M3 - Article
C2 - 8862596
AN - SCOPUS:0029777354
SN - 0095-1137
VL - 34
SP - 2267
EP - 2269
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 9
ER -