Comparison of different assays to assess human papillomavirus (HPV) type 16- and 18-specific antibodies after HPV infection and vaccination

Mirte Scherpenisse; Rutger M Schepp; Madelief Mollers; Sofie H Mooij; Chris J L M Meijer; Guy A M Berbers; Fiona R M van der Klis

doi:10.1128/CVI.00153-13

Comparison of different assays to assess human papillomavirus (HPV) type 16- and 18-specific antibodies after HPV infection and vaccination

Mirte Scherpenisse^*, Rutger M Schepp, Madelief Mollers, Sofie H Mooij, Chris J L M Meijer, Guy A M Berbers, Fiona R M van der Klis

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We compared the measurement of human papillomavirus (HPV)-specific serum antibody levels with the virus-like-particle multiplex immunoassay (VLP-MIA), competitive Luminex immunoassay (cLIA), and glutathione S-transferase (GST) L1-based MIA. Using a large panel of serum samples, these assays showed mutually good correlations for both naturally induced and vaccine-derived HPV-specific antibody levels. However, an adaptation of the GST L1-based MIA resulted in an improved correlation with both cLIA and VLP-MIA.

Original language	English
Pages (from-to)	1329-1332
Number of pages	4
Journal	Clinical and vaccine immunology : CVI
Volume	20
Issue number	8
DOIs	https://doi.org/10.1128/CVI.00153-13
Publication status	Published - Aug 2013
Externally published	Yes

Keywords

Antibodies, Viral/blood
Antigens, Viral
Clinical Laboratory Techniques/methods
Human papillomavirus 16/immunology
Human papillomavirus 18/immunology
Humans
Immunoassay/methods
Papillomavirus Infections/immunology
Papillomavirus Vaccines/administration & dosage
Virosomes

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10.1128/CVI.00153-13

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title = "Comparison of different assays to assess human papillomavirus (HPV) type 16- and 18-specific antibodies after HPV infection and vaccination",

abstract = "We compared the measurement of human papillomavirus (HPV)-specific serum antibody levels with the virus-like-particle multiplex immunoassay (VLP-MIA), competitive Luminex immunoassay (cLIA), and glutathione S-transferase (GST) L1-based MIA. Using a large panel of serum samples, these assays showed mutually good correlations for both naturally induced and vaccine-derived HPV-specific antibody levels. However, an adaptation of the GST L1-based MIA resulted in an improved correlation with both cLIA and VLP-MIA.",

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author = "Mirte Scherpenisse and Schepp, {Rutger M} and Madelief Mollers and Mooij, {Sofie H} and Meijer, {Chris J L M} and Berbers, {Guy A M} and {van der Klis}, {Fiona R M}",

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language = "English",

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AU - Scherpenisse, Mirte

AU - Schepp, Rutger M

AU - Mollers, Madelief

AU - Mooij, Sofie H

AU - Meijer, Chris J L M

AU - Berbers, Guy A M

AU - van der Klis, Fiona R M

PY - 2013/8

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N2 - We compared the measurement of human papillomavirus (HPV)-specific serum antibody levels with the virus-like-particle multiplex immunoassay (VLP-MIA), competitive Luminex immunoassay (cLIA), and glutathione S-transferase (GST) L1-based MIA. Using a large panel of serum samples, these assays showed mutually good correlations for both naturally induced and vaccine-derived HPV-specific antibody levels. However, an adaptation of the GST L1-based MIA resulted in an improved correlation with both cLIA and VLP-MIA.

AB - We compared the measurement of human papillomavirus (HPV)-specific serum antibody levels with the virus-like-particle multiplex immunoassay (VLP-MIA), competitive Luminex immunoassay (cLIA), and glutathione S-transferase (GST) L1-based MIA. Using a large panel of serum samples, these assays showed mutually good correlations for both naturally induced and vaccine-derived HPV-specific antibody levels. However, an adaptation of the GST L1-based MIA resulted in an improved correlation with both cLIA and VLP-MIA.

KW - Antibodies, Viral/blood

KW - Antigens, Viral

KW - Clinical Laboratory Techniques/methods

KW - Human papillomavirus 16/immunology

KW - Human papillomavirus 18/immunology

KW - Humans

KW - Immunoassay/methods

KW - Papillomavirus Infections/immunology

KW - Papillomavirus Vaccines/administration & dosage

KW - Virosomes

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DO - 10.1128/CVI.00153-13

M3 - Article

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SN - 1556-679X

VL - 20

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JO - Clinical and vaccine immunology : CVI

JF - Clinical and vaccine immunology : CVI

IS - 8

ER -

Comparison of different assays to assess human papillomavirus (HPV) type 16- and 18-specific antibodies after HPV infection and vaccination

Abstract

Keywords

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