TY - JOUR
T1 - Comparative immunohistochemical investigation of rat and human hepatocellular carcinomas
AU - Thoolen, Bob
AU - Ten Kate, Fiebo J.W.
AU - Castigliego, Domenico
AU - van Diest, Paul J.
AU - Malarkey, David E.
AU - Elmore, Susan A.
AU - Maronpot, Robert R.
PY - 2013/9/1
Y1 - 2013/9/1
N2 - Previously identified comparable morphological features of human and rat hepatoproliferative lesions were identified, including hepatocellular carcinomas (HCCs). In this study we identified similarities and differences in immunohistochemical (IHC) detection of some key proteins important in carcinogenesis that may link pathogenesis pathways of human and rat HCC. The comparative features of six IHC markers (Ki67, beta-catenin, CD34, glutamine synthetase (GS), c-myc, and transforming growth factor alpha (TGFalpha)), previously shown to be positive or altered in rodent and human HCC when compared to normal hepatocytes, were investigated. Glutamine synthetase (a hepatocellular enzyme) was strongly positive in 5/ 5 (100%) human and 4/5 (80%) rat HCCs examined. CD34 (an endothelial marker) and Ki-67 (a cell proliferation marker) were consistently positive in five human HCCs (5/5 for both markers) but weakly positive in only 2/5 and 3/5 rat HCCs, respectively. Beta-catenin, c-myc, and TGF-alpha were infrequently altered, with immunopositivity found in only one or two of either rat or human HCCs (a total of five each examined; beta-catenin: 1/5 rat samples, c-myc: 2/5 human samples, TGF-alpha: 1/5 rat samples positive). It was concluded that Ki-67, CD34, and GS, are most likely to be useful in studying the pathogenesis of HCC in rats and humans.
AB - Previously identified comparable morphological features of human and rat hepatoproliferative lesions were identified, including hepatocellular carcinomas (HCCs). In this study we identified similarities and differences in immunohistochemical (IHC) detection of some key proteins important in carcinogenesis that may link pathogenesis pathways of human and rat HCC. The comparative features of six IHC markers (Ki67, beta-catenin, CD34, glutamine synthetase (GS), c-myc, and transforming growth factor alpha (TGFalpha)), previously shown to be positive or altered in rodent and human HCC when compared to normal hepatocytes, were investigated. Glutamine synthetase (a hepatocellular enzyme) was strongly positive in 5/ 5 (100%) human and 4/5 (80%) rat HCCs examined. CD34 (an endothelial marker) and Ki-67 (a cell proliferation marker) were consistently positive in five human HCCs (5/5 for both markers) but weakly positive in only 2/5 and 3/5 rat HCCs, respectively. Beta-catenin, c-myc, and TGF-alpha were infrequently altered, with immunopositivity found in only one or two of either rat or human HCCs (a total of five each examined; beta-catenin: 1/5 rat samples, c-myc: 2/5 human samples, TGF-alpha: 1/5 rat samples positive). It was concluded that Ki-67, CD34, and GS, are most likely to be useful in studying the pathogenesis of HCC in rats and humans.
KW - Beta-catenin
KW - c-myc
KW - Carcinogenesis
KW - CD34
KW - Glutamine synthetase
KW - Hepatocellular carcinoma
KW - Human
KW - Immunohistochemistry
KW - Ki-67
KW - Liver tumors
KW - Rat
KW - TGF-alpha
UR - https://www.scopus.com/pages/publications/84884143347
U2 - 10.1179/2046023613Y.0000000026
DO - 10.1179/2046023613Y.0000000026
M3 - Article
AN - SCOPUS:84884143347
SN - 0147-8885
VL - 36
SP - 75
EP - 85
JO - Journal of Histotechnology
JF - Journal of Histotechnology
IS - 3
ER -